PHGDH (NM_006623) Human Untagged Clone

CAT#: SC322244

PHGDH (untagged)-Human phosphoglycerate dehydrogenase (PHGDH)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PHGDH
• Synonyms: 3-PGDH; 3PGDH; HEL-S-113; NLS; NLS1; PDG; PGAD; PGD; PGDH; PHGDHD; SERA
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322244 GAGGAGGAGGAGGAGATGACTGGGGAGCGGGAGCTCGAGAATACTGCCCAGTTACTCTAG
  CGCGCCAGGCCGAACCGCAGCTTCTTGGCTTAGGTACTTCTACTCACAGCGGCCGATTCC
  GAGGCCAACTCCAGCAATGGCTTTTGCAAATCTGCGGAAAGTGCTCATCAGTGACAGCCT
  GGACCCTTGCTGCCGGAAGATCTTGCAAGATGGAGGGCTGCAGGTGGTGGAAAAGCAGAA
  CCTTAGCAAAGAGGAGCTGATAGCGGAGCTGCAGGACTGTGAAGGCCTTATTGTTCGCTC
  TGCCACCAAGGTGACCGCTGATGTCATCAACGCAGCTGAGAAACTCCAGGTGGTGGGCAG
  GGCTGGCACAGGTGTGGACAATGTGGATCTGGAGGCCGCAACAAGGAAGGGCATCTTGGT
  TATGAACACCCCCAATGGGAACAGCCTCAGTGCCGCAGAACTCACTTGTGGAATGATCAT
  GTGCCTGGCCAGGCAGATTCCCCAGGCGACGGCTTCGATGAAGGACGGCAAATGGGAGCG
  GAAGAAGTTCATGGGAACAGAGCTGAATGGAAAGACCCTGGGAATTCTTGGCCTGGGCAG
  GATTGGGAGAGAGGTAGCTACCCGGATGCAGTCCTTTGGGATGAAGACTATAGGGTATGA
  CCCCATCATTTCCCCAGAGGTCTCGGCCTCCTTTGGTGTTCAGCAGCTGCCCCTGGAGGA
  GATCTGGCCTCTCTGTGATTTCATCACTGTGCACACTCCTCTCCTGCCCTCCACGACAGG
  CTTGCTGAATGACAACACCTTTGCCCAGTGCAAGAAGGGGGTGCGTGTGGTGAACTGTGC
  CCGTGGAGGGATCGTGGACGAAGGCGCCCTGCTCCGGGCCCTGCAGTCTGGCCAGTGTGC
  CGGGGCTGCACTGGACGTGTTTACGGAAGAGCCGCCACGGGACCGGGCCTTGGTGGACCA
  TGAGAATGTCATCAGCTGTCCCCACCTGGGTGCCAGCACCAAGGAGGCTCAGAGCCGCTG
  TGGGGAGGAAATTGCTGTTCAGTTCGTGGACATGGTGAAGGGGAAATCTCTCACGGGGGT
  TGTGAATGCCCAGGCCCTTACCAGTGCCTTCTCTCCACACACCAAGCCTTGGATTGGTCT
  GGCAGAAGCTCTGGGGACACTGATGCGAGCCTGGGCTGGGTCCCCCAAAGGGACCATCCA
  GGTGATAACACAGGGAACATCCCTGAAGAATGCTGGGAACTGCCTAAGCCCCGCAGTCAT
  TGTCGGCCTCCTGAAAGAGGCTTCCAAGCAGGCGGATGTGAACTTGGTGAACGCTAAGCT
  GCTGGTGAAAGAGGCTGGCCTCAATGTCACCACCTCCCACAGCCCTGCTGCACCAGGGGA
  GCAAGGCTTCGGGGAATGCCTCCTGGCCGTGGCCCTGGCAGGCGCCCCTTACCAGGCTGT
  GGGCTTGGTCCAAGGCACTACACCTGTACTGCAGGGGCTCAATGGAGCTGTCTTCAGGCC
  AGAAGTGCCTCTCCGCAGGGACCTGCCCCTGCTCCTATTCCGGACTCAGACCTCTGACCC
  TGCAATGCTGCCTACCATGATTGGCCTCCTGGCAGAGGCAGGCGTGCGGCTGCTGTCCTA
  CCAGACTTCACTGGTGTCAGATGGGGAGACCTGGCACGTCATGGGCATCTCCTCCTTGCT
  GCCCAGCCTGGAAGCGTGGAAGCAGCATGTGACTGAAGCCTTCCAGTTCCACTTCTAACC
  TTGGAGCTCACTGGTCCCTGCCTCTGGGGCTTTTCTGAAGAAACCCACCCACTGTGATCA
  ATAGGGAGAGAAAATCCACATTCTTGGGCTGAACGCGGGCCTCTGACACTGCTTACACTG
  CACTCTGACCCTGTAGTACAGCAATAACCGTCTAATAAAGAGCCTACCCCCAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for SC322244 unedited
  GGCCATTGTATACGACTCCTATAGGGCGGCCGGCGATCGCGGCGCGCCGGTACCGGTCCG
  GAATTCCCGGGATATCGTCGACCCACGCGTCCGGAGGAGGAGGAGGAGATGACTGGGGAG
  CGGGAGCTCGAGAATACTGCCCAGTTACTCTAGCGCGCCAGGCCGAACCGCAGCTTCTTG
  GCTTAGGTACTTCTACTCACAGCGGCCGATTCCGAGGCCAACTCCAGCAATGGCTTTTGC
  AAATCTGCGGAAAGTGCTCATCAGTGACAGCCTGGACCCTTGCTGCCGGAAGATCTTGCA
  AGATGGAGGGCTGCAGGTGGTGGAAAAGCAGAACCTTAGCAAAGAGGAGCTGATAGCGGA
  GCTGCAGGACTGTGAAGGCCTTATTGTTCGCTCTGCCACCAAGGTGACCGCTGATGTCAT
  CAACGCAGCTGAGAAACTCCAGGTGGTGGGCAGGGCTGGCACAGGTGTGGACAATGTGGA
  TCTGGAGGCCGCAACAAGGAAGGGCATCTTGGTTATGAACACCCCCAATGGGAACAGCCT
  CAGTGCCGCAGAACTCACTTGTGGAATGATCATGTGCCTGGCCAGGCAGATTCCCCAGGC
  GACGGCTTCGATGTGACGGCAAATGGGAGCGGAGAGTTCATGGGAACAGAGCTGATGGAA
  AGACCCTGGGAATTCTTGGCCTGGGCAGGATTGGGAGAGAGGTAGCTACCCGGATGCAGT
  CCTTTGGGATGAGACTATAGGGTATGACCCCATCATTTCCCCAGAGGTCTCGGCCTCCTT
  TGTGTTCAGCAGCTGCCCTGAGGAGATCTGGCCTCTCTGTGATTTCATCACTGTGCACCA
  CTCCTCTCCTGCCCTCCACGACAGGCTGCTGATGACAACACCTTGGCCAATGCAGAGGGG
  GTGGCGGGTGGGTGACTTGGTGCCGTTGGAGGAATCCGTGACAAAGGCCCCCTGTCTCCG
  C
• Restriction Sites: Please inquire
• ACCN: NM_006623
• ORF Size: 1602 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_006623.2, NP_006614.2
• RefSeq Size: 1968
• RefSeq ORF: 1602
• Locus ID: 26227
• Domains: 2-Hacid_DH, 2-Hacid_DH_C
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Glycine, serine and threonine metabolism, Metabolic pathways
• Gene Summary: This gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known. [provided by RefSeq, Aug 2011]