Placental lactogen (CSH2) (NM_022644) Human Untagged Clone

CAT#: SC127020

CSH2 (untagged)-Human chorionic somatomammotropin hormone 2 (CSH2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CSH2
• Synonyms: CS-2; CSB; GHB1; hCS-B; PL
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_022644, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTGCAGGCTCCCGGACGTCCCTGCTCCTGGCTTTTGCCCTGCTCTGCCTGCCCTGGCTTCAAGAGG
  CTGGTGCCGTCCAAACCGTTCCGTTATCCAGGCTTTTTGACCACGCTATGCTCCAAGCCCATCGCGCGCA
  CCAGCTGGCCATTGACACCTACCAGGAGTTTGAAGAAACCTATATCCCAAAGGACCAGAAGTATTCATTC
  CTGCATGACTCCCAGACCTCCTTCTGCTTCTCAGACTCTATTCCGACACCCTCCAACATGGAGGAAACGC
  AACAGAAATCCAATCTAGAGCTGCTCCGCATCTCCCTGCTGCTCATCGAGTCGTGGCTGGAGCCCGTGCG
  GTTCCTCAGGAGTATGTTCGCCAACAACCTGGTGTATGACACCTCGGACAGCGATGACTATCACCTCCTA
  AAGGACCTAGAGGAAGGCATCCAAACGCTGATGGGGGTGAGGGTGGCGCCAGGGGTCGCCAATCCTGGAA
  CCCCACTGGCTTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022644 unedited
  CCCCGGGCCCCCNNNNNNNNNTNNNCCCNCCCCCGCCCGTCAAATTGTAACGACCACTAA
  GGCGGCCGGAATCGCCGACCCGACCACTCAGGTCCTGTGGACAGCTCACCTAGTGGCAAT
  GGCTCCAGGCTCCCGGACGTCCCTGCTCCTGGCTTTTGCCCTGCTCTGCCTGCCCTGGCT
  TCAAGAGGCTGGTGCCGTCCAAACCGTTCCGTTATCCAGGCTTTTTGACCACGCTATGCT
  CCAAGCCCATCGCGCGCACCAGCTGGCCATTGACACCTACCAGGAGTTTGAAGAAACCTA
  TATCCCAAAGGACCAGAAGTATTCATTCCTGCATGACTCCCAGACCTCCTTCTGCTTCTC
  AGACTCTATTCCGACACCCTCCAACATGGAGGAAACGCAACAGAAATCCAATCTAGAGCT
  GCTCCGCATCTCCCTGCTGCTCATCGAGTCGTGGCTGGAGCCCGTGCGGTTCCTCAGGAG
  TATGTTCGCCAACAACCTGGTGTATGACACCTCGGACAGCGATGACTATCACCTCCTAAA
  GGACCTAGAGGAAGGCATCCAAACGCTGATGGGGGTGAGGGTGGCGCCAGGGGTCACCAA
  TCCTGGAACCCCACTGGCTTCGAGGGCTGGGGGAGAAGAATACTGCTGCCCTCTTTNTAG
  CAGTAAGGCGCTGACCCAAGAGAACTCACCTTATTCTTCATTTCGCCTGGTGAATCCTNC
  AGGCCTTTCTCTACACCCTGAAGGGAGGGANGAAAATGATAAAATGAGAGAAGGAGGGAA
  CAGTGCCCAAGCGCTTGGCCTCTCCTTCTCTTCCCTCACTTTGCAAAGGCTGGAAAACGG
  CAGCCCGCNGNACTGGGCAGATNCCTCAGCAGACCTACAGCCAGTTTTGCCACAAACTCG
  CACAACCATGACGCACTGCTCAAAGACTACGGGCTGCTCTACTGCTTCAGAAAGGACATG
  GACCAAGTCNAGAAAATTCTGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_022644
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022644.2, NP_072170.1
• RefSeq Size: 1134 bp
• RefSeq ORF: 504 bp
• Locus ID: 1443
• Cytogenetics: 17q23.3
• Protein Families: Secreted Protein
• Gene Summary: 'The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones and plays an important role in growth control. The gene is located at the growth hormone locus on chromosome 17 along with four other related genes in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. Although the five genes share a remarkably high degree of sequence identity, they are expressed selectively in different tissues. Alternative splicing generates additional isoforms of each of the five growth hormones. This particular family member is expressed mainly in the placenta and utilizes multiple transcription initiation sites. Expression of the identical mature proteins for chorionic somatomammotropin hormones 1 and 2 is upregulated during development, while the ratio of 1 to 2 increases by term. Structural and expression differences provide avenues for developmental regulation and tissue specificity. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) contains intron D and encodes an isoform (2) that diverges from all other CS isoforms in the carboxy-terminus due to a frameshift. Early truncation occurs relative to a similarly spliced variant of chorionic somatomammotropin hormone 1.