PEDF (SERPINF1) (M76979) Human Untagged Clone

CAT#: SC127277

(untagged)-H.sapiens pigment epithelium-differentiation factor (PEDF) mRNA, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SERPINF1
• Synonyms: EPC-1|OI12|OI6|PEDF|PIG35
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127277 sequence for M76979 edited (data generated by NextGen Sequencing)
  ATGCAGGCCCTGGTGCTACTCCTCTGCATTGGAGCCCTCCTCGGGCACAGCAGCTGCCAG
  AACCCTGCCAGCCCCCCGGAGGAGGGCTCCCCAGACCCCGACAGCACAGGGGCGCTGGTG
  GAGGAGGAGGATCCTTTCTTCAAAGTCCCCGTGAACAAGCTGGCAGCGGCTGTCTCCAAC
  TTCGGCTATGACCTGTACCGGGTGCGATCCAGCACGAGCCCCACGACCAACGTGCTCCTG
  TCTCCTCTCAGTGTGGCCACGGCCCTCTCGGCCCTCTCGCTGGGAGCGGANNAGCGAACA
  GAATCCATCATTCACCGGGCTCTCTACTATGACTTGATCAGCAGCCCAGACATCCATGGT
  ACCTATAAGGAGCTCCTTGACACGGTCACTGCCCCCCAGAAGAACCTCAAGAGTGCCTCC
  CGGATCGTCTTTGAGAAGAAGCTGCGCATAAAATCCAGCTTTGTGGCACCTCTGGAAAAG
  TCATATGGGACCAGGCCCAGAGTCCTGACGGGCAACCCTCGCTTGGACCTGCAAGAGATC
  AACAACTGGGTGCAGGCGCAGATGAAAGGGAAGCTCGCCAGGTCCACAAAGGAAATTCCC
  GATGAGATCAGCATTCTCCTTCTCGGTGTGGCGCACTTCAAGGGGCAGTGGGTAACAAAG
  TTTGACTCCAGAAAGACTTCCCTCGAGGATTTCTACTTGGATGAAGAGAGGACCGTGAGG
  GTCCCCATGATGTCGGACCCTAAGGCTGTTTTACGCTATGGCTTGGATTCAGATCTCAGC
  TGCAAGATTGCCCAGCTGCCCTTGACCGGAAGCATGAGTATCATCTTCTTCCTGCCCCTG
  AAAGTGACCCAGAATTTGACCTTGATAGAGGAGAGCCTCACCTCCGAGTTCATTCATGAC
  ATAGACCGAGAACTGAAGACCGTGCAGGCGGTCCTCACTGTCCCCAAGCTGAAGCTGAGT
  TATGAAGGCGAAGTCACCAAGTCCCTGCAGGAGATGAAGCTGCAATCCTTGTTTGATTCA
  CCAGACTTTAGCAAGATCACAGGCAAACCCATCAAGCTGACTCAGGTGGAACACCGGGCT
  GGCTTTGAGTGGAACGAGGATGGGGCGGGAACCACCCCCAGCCCAGGGCTGCAGCCTGCC
  CACCTCACCTTCCCGCTGGACTATCACCTTAACCAGCCTTTCATCTTCGTACTGAGGGAC
  ACAGACACAGGGGCCCTTCTCTTCATTGGCAAGATTCTGGACCCCAGGGGCCCCTAA
  
  Clone variation with respect to M76979.1
  215 t=>c;291 c=>n;292 g=>n;444 r=>g;963 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for M76979 unedited
  TTTTGTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGGGGTGTGGAGCTG
  CAGCGTATCCACAGGCCCCAGGATGCAGGCCCTGGTGCTACTCCTCTGCATTGGAGCCCT
  CCTCGGGCACAGCAGCTGCCAGAACCCTGCCAGCCCCCCGGAGGAGGGCTCCCCAGACCC
  CGACAGCACAGGGGCGCTGGTGGAGGAGGAGGATCCTTTCTTCAAAGTCCCCGTGAACAA
  GCTGGCAGCGGCTGTCTCCAACTTCGGCTATGACCTGTACCGGGTGCGATCCAGCACGAG
  CCCCACGACCAACGTGCTCCTGTCTCCTCTCAGTGTGGCCACGGCCCTCTCGGCCCTCTC
  GCTGGGAGCGGAGCAGCGAACAGAATCCATCATTCACCGGGCTCTCTACTATGACTTGAT
  CAGCAGCCCAGACATCCATGGTACCTATAAGGAGCTCCTTGACACGGTCACTGCCCCCCA
  GAAGAACCTCAAGAGTGCCTCCCGGATCGTCTTTGAGAAGAAGCTGCGCATAAAATCCAG
  CTTTGTGGCACCTCTGGAAAAGTCATATGGGACCAGGCCCAGAGTCCTGACGGGCAACCC
  TCGCTTGGACCTGCAAGAGATCAACAACTGGGTGCAGGCGCAGATGAAAGGGAAGCTCGC
  CAGGTCCACAAAGGAAATTCCCGATGAGATCAGCATTCTCCTTCTCGGTGTGGCGCACTT
  CAGGGGGCAGTGGGTAACAAAGTTTGACTCCAGAAAGACTTCCCTCGAGGATNTCTACTT
  GGATGAAGAGAAGGACCCGTGAGGGTCCNCATGATGTCNGACCCTANGGCTGNNTTACGC
  TATGGCTGGNATTCAGATCTCAGCTGCAAGATTGCCCAGCTGCCCTTGACCGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for M76979 unedited
  TAGAGTCGAGTTTTTTTTTTTTTTTTTTAGGGATAAAGCTCTTTTATTGTATGCATTGAA
  ACCTTACAGGGGCAGCCTTCGTGTCCTGTGGAATCTGCTGTCCCTCGGGTTTTCTTCTAG
  GGTATTGGAATATTAAACTGGGATATTAGGGGCCCCTGGGGTCCAGAATCTTGCCAATGA
  AGAGAAGGGCCCCTGTGTCTGTGTCCCTCAGTACGAAGATGAAAGGCTGGTTAAGGTGAT
  AGTCCAGCGGGAAGGTGAGGTGGGCAGGCTGCAGCCCTGGGCTGGGGGTGGTTCCCGCCC
  CATCCTCGTTCCACTCAAAGCCAGCCCGGTGTTCCACCTGAGTCAGCTTGATGGGTTTGC
  CTGTGATCTTGCTAAAGTCTGGTGAATCAAACAAGGATTGCAGCTTCATCTCCTGCAGGG
  ACTTGGTGACTTCGCCTTCATAACTCAGCTTCAGCTTGGGGACAGTGAGGACCGCCTGCA
  CGGTCTTCAGTTCTCGGTCTATGTCATGAATGAACTCGGAGGTGAGGCTCTCCTCTATCA
  AGGTCAAATTCTGGGTCACTTTCAGGNGCANGAAAGAGATGATACTCATGCTTCCGGGTC
  AGGGCAGCTGGGCAATCTTGCAGCTGAGATCTGAATCCCAGCATAGCGTAAACAGCCTTA
  GGGTCGACATATGGGACCCTCACGTCTCTCTCATCAGTAAAATCTCGAGGAAGTCTTCTG
  AGTAAACTTTGTACCACTGCCCTTGAGTGCGCACACGAGAGGAAATGCTGATCTATCGGA
  ATTNCTTTGTGGACTGGGAGCTTCCTTCATCTGCGCTGACCCATTGTGGACTCTGCAGGT
  CAACCAGGGTGCCGTCAGACTCTGCCCTGTCATTGACTTTTCAAGTGCACAAGCTGATTT
  ATGCCACCTCTTTCAAACATCGGAGCCTTTGAGTTCTTGGGGGCNGACGGTAAGACTCTT
  AGCCCAGAGTTGGCTTGACATCTAAAACCGGGATTAGTTCTTCA
  
• Restriction Sites: NotI-NotI
• ACCN: M76979
• Insert Size: 1420 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: M76979.1, AAA60058.1
• RefSeq Size: 1490 bp
• RefSeq ORF: 1257 bp
• Locus ID: 5176
• Cytogenetics: 17p13.3
• Domains: SERPIN
• Protein Families: Druggable Genome, Secreted Protein
• Gene Summary: 'This gene encodes a member of the serpin family that does not display the serine protease inhibitory activity shown by many of the other serpin proteins. The encoded protein is secreted and strongly inhibits angiogenesis. In addition, this protein is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells. Mutations in this gene were found in individuals with osteogenesis imperfecta, type VI. [provided by RefSeq, Aug 2016]'