Sterol carrier protein 2 (SCP2) (NM_002979) Human Untagged Clone

CAT#: SC127431

SCP2 (untagged)-Human sterol carrier protein 2 (SCP2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SCP2
• Synonyms: NLTP; NSL-TP; SCP-2; SCP-CHI; SCP-X; SCPX
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127431 sequence for NM_002979 edited (data generated by NextGen Sequencing)
  ATGTCCTCTTCCCCGTGGGAGCCTGCGACCCTGCGCCGGGTGTTCGTGGTGGGGGTTGGC
  ATGACCAAGTTTGTGAAGCCTGGAGCTGAGAATTCAAGAGACTACCCTGACTTGGCAGAA
  GAAGCAGGCAAGAAGGCTTTAGCTGATGCACAGATCCCTTATTCAGCAGTGGACCAGGCA
  TGTGTTGGCTATGTTTTTGGTGACTCTACCTGTGGGCAGAGGGCTATCTATCACAGTTTG
  GGAATGACTGGAATTCCTATAATCAATGTCAACAATAACTGTGCTACTGGTTCTACTGCT
  TTGTTTATGGCCCGCCAGCTGATTCAGGGTGGTGTGGCAGAATGTGTCTTGGCTCTTGGG
  TTTGAGAAGATGAGTAAGGGAAGCCTTGGAATAAAATTTTCAGATAGAACCATTCCCACT
  GATAAGCATGTTGACCTCCTGATCAATAAGTATGGATTGTCTGCTCACCCAGTTGCTCCT
  CAGATGTTTGGGTATGCTGGAAAAGAACATATGGAAAAATATGGAACAAAAATTGAACAC
  TTTGCAAAAATTGGATGGAAAAATCATAAACATTCAGTTAATAACCCGTATTCCCAGTTC
  CAAGATGAGTACAGTTTAGATGAAGTGATGGCATCTAAAGAAGTTTTTGATTTTTTGACT
  ATCTTACAATGTTGTCCCACTTCAGATGGTGCTGCAGCAGCAATTTTGGCCAGTGAAGCA
  TTTGTACAGAAGTATGGCCTGCAATCCAAAGCTGTGGAAATTTTGGCACAAGAAATGATG
  ACTGATTTGCCAAGCTCGTTTGAAGAAAAAAGCATTATTAAAATGGTTGGCTTTGATATG
  AGTAAAGAAGCTGCAAGAAAATGCTATGAGAAATCTGGCCTGACACCAAATGATATTGAC
  GTAATAGAACTTCACGATTGCTTTTCTACCAACGAACTCCTTACTTATGAAGCACTGGGA
  CTCTGTCCAGAAGGACAAGGTGCAACGCTGGTTGATAGAGGAGATAATACATATGGAGGA
  AAGTGGGTCATAAATCCTAGTGGTGGACTGATTTCAAAGGGACACCCACTAGGCGCTACA
  GGTCTTGCTCAGTGTGCAGAACTCTGCTGGCAGCTGAGAGGGGAAGCCGGAAAGAGGCAA
  GTTCCTGGTGCAAAGGTGGCTCTGCAGCATAATTTAGGCATTGGAGGAGCTGTGGTTGTA
  ACACTCTACAAGATGGGTTTTCCGGAAGCCGCCAGTTCTTTTAGAACTCATCAAATTGAA
  GCTGTTCCAACCAGCTCTGCAAGTGATGGATTTAAGGCAAATCTTGTTTTTAAGGAGATT
  GAGAAGAAACTTGAAGAGGAAGGGGAACAGTTTGTGAAGAAAATCGGTGGTATTTTTGCC
  TTCAAGGTGAAAGATGGCCCTGGGGGTAAAGAGGCCACCTGGGTGGTGGATGTGAAGAAT
  GGCAAAGGATCAGTGCTTCCTAACTCAGATAAGAAGGCTGACTGCACAATCACAATGGCT
  GACTCAGACTTCCTGGCTTTAATGACTGGTAAAATGAATCCTCAGTCGGCCTTCTTTCAA
  GGCAAATTGAAAATCACTGGCAACATGGGTCTCGCTATGAAGTTACAAAATCTTCAGCTT
  CAGCCAGGCAACGCTAAGCTCTGA
  
  Clone variation with respect to NM_002979.4
  609 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002979 unedited
  GGATTTTGTAATACGACTTACTATAGGGNNCGGCCGCGCAATTCGGCACGAGGTCTCCGC
  CTGTCAGTGCCGGCAGTCGTCCGCGGCGCCCGCCCCGGTCCCGCACTGGTGCAGCCATGT
  CCTCTTCCCCGTGGGAGCCTGCGACCCTGCGCCGGGTGTTCGTGGTGGGGGTTGGCATGA
  CCAAGTTTGTGAAGCCTGGAGCTGAGAATTCAAGAGACTACCCTGACTTGGCAGAAGAAG
  CAGGCAAGAAGGCTTTAGCTGATGCACAGATCCCTTATTCAGCAGTGGACCAGGCATGTG
  TTGGCTATGTTTTTGGTGACTCTACCTGTGGGCAGAGGGCTATCTATCACAGTTTGGGAA
  TGACTGGAATTCCTATAATCAATGTCAACAATAACTGTGCTACTGGTTCTACTGCTTTGT
  TTATGGCCCGCCAGCTGATTCAGGGTGGTGTGGCAGAATGTGTCTTGGCTCTTGGGTTTG
  AGAAGATGAGTAAGGGAAGCCTTGGAATAAAATTTTCAGATAGAACCATTCCCACTGATA
  AGCATGTTGACCTCCTGATCAATAAGTATGGATTGTCTGCTCACCCAGTTGCTCCTCAGA
  TGTTTGGGTATGCTGGGAAAGACATATGGAAAAATATGGAACANAAATTGAACACTTTGC
  ANAAATTGGATGGAAAAATCATANACATTCAGTTAAATACCCGTATTCCCAGTTCCAAGA
  TGAGTACAGTTTAGATGAAGTGATGGCATCTAAAGAAGTTTTTGATTTTTTGACTATNCT
  TACATGTTGTCCCACTTCAGATGGTGCTGCNAGCAGCATTTTGGGCAGTGAAGCATTTGT
  ACAGAAGTATGGCCTGCAATCCAAGCTGTTGAAATTTTGGCACCAGAAATGATGACTGAT
  TTGGCAAAGCTCGTTTGAGGAAAAAGCATTATTAAATGGNTGGCTTTGGATTGAGTAANG
  AAGCTGCCAGAAATGCTTTGAGAAATCTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002979 unedited
  GTACGACTATGNNACCGCGGCCGCAATCTATGATCGAGTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTGACGCCCGAAAAGTTATTTTAATTTTCTATTAAACATTCTTCTCAAAGCA
  TTATTTTATCCTATATCTCACTGAATTTTAAGAAATAACATTAGGATTAGAAAAACTAGG
  AAAAAAGATAAATGCCGATAATTAAACTTACCTGAAAAAGGAAAATTATAACCAAGGACT
  GAGAACGTTATAAATTGGAATGAGATTATAATTTGAAAACTGCATCTGAAAGCAAACTTT
  ATTGGTCAATTATTCTTAATGATGGGGTTTTATGACTAATACACTGGTTTTTCAAGAAGG
  AAACCCATGTTAAAAATATTTTTATTTTAAAAATAAGCCTGTGTTCAAGCTCTGATCATA
  TTTCTTTTATTTTGATTTGGGAAGAAAATACCGGTTCTGATAGCATGAAATGCAAAATTT
  TTAGATTTTTAATCTCACTAATTTTAAGAACTATTGAGAAATTGATTAATGACATGGAGT
  GCACAACACTAATTACTGGCCAGCTGTTGGCATTGCGTTTCTTACTTAGTTCTCCCAAGG
  AAAACTCTTAAATTGAATCTTCAGCAGAATATCCTTAAAATGCTTTGTTAGCCAACAAAA
  GCTTTTTTGATTACATAGTTCTTTGGATTTTACTGTTCCTAATTTTATTCTGGAACTCAA
  TTTTACCCCAGACCATGATTACCATATTAACTTTGTNATGCACAGTTGTATTGCATTCCC
  GCAAAGCAGTAGTTACCCTCCGGCTCTTTTCCCCCCGACCTTGGAAAACAGGGTTGGGCC
  CACCCATTAGAAACAATCTTTCCCCGCCATTGGCATAGGGGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002979
• Insert Size: 2750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002979.3, NP_002970.2
• RefSeq Size: 2697 bp
• RefSeq ORF: 1644 bp
• Locus ID: 6342
• Cytogenetics: 1p32.3
• Domains: thiolase, SCP2
• Protein Pathways: Metabolic pathways, PPAR signaling pathway, Primary bile acid biosynthesis
• Gene Summary: 'This gene encodes two proteins: sterol carrier protein X (SCPx) and sterol carrier protein 2 (SCP2), as a result of transcription initiation from 2 independently regulated promoters. The transcript initiated from the proximal promoter encodes the longer SCPx protein, and the transcript initiated from the distal promoter encodes the shorter SCP2 protein, with the 2 proteins sharing a common C-terminus. Evidence suggests that the SCPx protein is a peroxisome-associated thiolase that is involved in the oxidation of branched chain fatty acids, while the SCP2 protein is thought to be an intracellular lipid transfer protein. This gene is highly expressed in organs involved in lipid metabolism, and may play a role in Zellweger syndrome, in which cells are deficient in peroxisomes and have impaired bile acid synthesis. Alternative splicing of this gene produces multiple transcript variants, some encoding different isoforms.[provided by RefSeq, Aug 2010]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1, also known as SCPx).