PHC1 (NM_004426) Human Untagged Clone

CAT#: SC127500

PHC1 (untagged)-Human polyhomeotic homolog 1 (Drosophila) (PHC1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PHC1
• Synonyms: EDR1; HPH1; MCPH11; RAE28
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127500 sequence for NM_004426 edited (data generated by NextGen Sequencing)
  ATGGAGACTGAGAGCGAGCAGAACTCCAATTCCACCAATGGGAGTTCTAGCTCAGGGGGC
  AGCTCTCGGCCCCAGATAGCTCAAATGTCACTATATGAACGACAAGCAGTGCAGGCTCTG
  CAAGCACTGCAGCGGCAGCCCAATGCAGCTCAGTATTTCCACCAGTTCATGCTCCAGCAG
  CAGCTCAGTAATGCCCAGCTGCATAGCCTGGCTGCCGTCCAGCAGGCCACAATTGCTGCC
  AGTCGGCAGGCCAGCTCCCCAAACACCAGCACTACACAGCAGCAGACTACCACCACCCAG
  GCCTCGATCAATCTGGCCACCACATCGGCCGCCCAGCTCATCAGCCGATCCCAGAGTGTG
  AGCTCTCCCAGTGCTACCACCTTGACCCAATCTGTGCTACTGGGGAACACCACCTCCCCA
  CCCCTCAACCAGTCTCAGGCCCAGATGTATCTACGGCCACAGCTGGGAAACCTATTGCAG
  GTAAACCGAACCCTGGGTCGGAATGTGCCTCTAGCCTCCCAACTCATCCTGATGCCTAAT
  GGGGCGGTGGCTGCAGTCCAGCAGGAGGTGCCATCTGCTCAGTCTCCTGGAGTTCATGCA
  GATGCAGATCAGGTTCAGAACTTGGCAGTAAGGAATCAACAGGCCTCAGCTCAAGGACCT
  CAGATGCAAGGCTCCACTCAGAAGGCCATTCCTCCAGGAGCCTCCCCTGTCTCTAGCCTC
  TCCCAGGCCTCTAGCCAGGCCCTAGCGGTGGCACAGGCTTCCTCTGGGGCCACAAACCAG
  TCCCTCAACCTTAGTCAAGCTGGTGGAGGCAGTGGGAATAGCATCCCAGGGTCCATGGGT
  CCAGGTGGAGGTGGGCAGGCACATGGTGGTTTGGGTCAGTTGCCTTCCTCAGGAATGGGT
  GGTGGGAGCTGTCCCAGGAAGGGTACAGGAGTGGTGCAGCCCTTGCCTGCAGCCCAAACA
  GTGACTGTGAGCCAGGGCAGCCAGACAGAGGCAGAAAGTGCAGCAGCCAAGAAGGCAGAA
  GCAGATGGGAGTGGCCAGCAGAATGTGGGCATGAACCTGACACGGACAGCCACACCTGCG
  CCCAGCCAGACACTTATTAGCTCAGCCACCTACACACAGATCCAGCCCCATTCACTGATT
  CAGCAACAGCAACAGATCCACCTCCAGCAGAAACAGGTGGTGATCCAGCAGCAGATTGCC
  ATCCACCACCAGCAGCAGTTCCAGCACCGGCAGTCCCAGCTCCTTCACACAGCTACACAC
  CTCCAGTTGGCGCAGCAGCAGCAGCAGCAACAACAGCAACAGCAGCAACAGCAGCAGCCG
  CAAGCCACCACCCTCACTGCCCCTCAGCCACCACAGGTCCCACCTACTCAGCAGGTCCCA
  CCTTCCCAGTCCCAGCAGCAAGCCCAAACCCTGGTCGTTCAGCCCATGCTTCAGTCTTCA
  CCCTTGTCTCTTCCACCTGATGCAGCCCCTAAGCCACCAATTCCCATCCAATCCAAACCA
  CCTGTAGCACCTATCAAGCCGCCTCAGTTAGGGGCCGCTAAGATGTCAGCTGCCCAGCAA
  CCACCACCCCATATCCCTGTGCAAGTTGTAGGCACTCGACAGCCAGGTACAGCCCAGGCA
  CAGGCTTTGGGGTTGGCACAGCTGGCAGCTGCTGTACCTACTTCCCGGGGGATGCCAGGT
  ACAGTGCAGTCTGGTCAGGCCCATTTGGCCTCCTCGCCACCTTCATCCCAGGCTCCTGGT
  GCACTGCAGGAGTGCCCTCCCACATTGGCCCCTGGGATGACCCTTGCTCCTGTGCAGGGG
  ACAGCACATGTGGTAAAGGGTGGGGCTACCACCTCCTCACCTGTTGTAGCCCAGGTCCCT
  GCTGCCTTCTATATGCAGTCTGTGCACTTGCCGGGTAAACCCCAGACATTGGCTGTCAAA
  CGCAAGGCTGACTCTGAGGAGGAGAGAGATGATGTCTCCACATTGGGTTCAATGCTTCCT
  GCCAAGGCATCTCCAGTAGCAGAAAGCCCAAAAGTCATGGACGAGAAGAGCAGTCTTGGA
  GAAAAAGCTGAATCAGTGGCTAATGTGAATGCTAATACTCCAAGCAGTGAACTAGTAGCC
  TTGACCCCCGCCCCTTCAGTACCGCCTCCTACACTAGCCATGGTGTCTAGACAAATGGGT
  GACTCAAAACCCCCACAGGCCATCGTGAAGCCCCAGATTCTCACCCACATCATTGAAGGC
  TTTGTTATCCAGGAAGGAGCAGAACCTTTCCCGGTGGGTTGTTCTCAGTTACTGAAGGAG
  TCTGAGAAGCCACTACAGACTGGCCTTCCGACAGGGCTGACTGAGAATCAGTCAGGTGGC
  CCTTTGGGAGTGGACAGCCCATCTGCTGAGTTAGATAAGAAGGCGAATCTCCTGAAGTGC
  GAGTACTGTGGGAAGTACGCCCCCGCAGAGCAGTTTCGTGGCTCTAAGAGGTTCTGCTCC
  ATGACTTGCGCTAAGAGGTACAATGTGAGCTGTAGCCATCAGTTCCGGCTGAAGAGGAAA
  AAAATGAAAGAGTTTCAAGAAGCCAACTATGCTCGCGTTCGCAGGCGTGGACCCCGCCGC
  AGCTCCTCTGACATTGCCCGTGCCAAGATTCAGGGCAAGTGCCACCGGGGTCAAGAAGAC
  TCTAGCCGGGGTTCAGATAATTCCAGTTATGATGAAGCACTCTCTCCAACATCTCCTGGG
  CCTTTATCAGTAAGAGCTGGGCATGGAGAACGTGACCTGGGGAATCCCAATACAGCTCCA
  CCTACACCGGAATTACATGGCATCAACCCTGTGTTCCTGTCCAGTAATCCCAGCCGTTGG
  AGTGTAGAGGAGGTGTACGAGTTTATTGCTTCTCTCCAAGGCTGCCAAGAGATTGCAGAG
  GAATTTCGCTCACAGGAGATTGATGGACAGGCCCTTTTATTACTTAAAGAAGAACATCTT
  ATGAGTGCCATGAACATCAAGCTGGGCCCTGCCCTCAAGATCTGCGCCAAGATAAATGTC
  CTCAAGGAGACCTAA
  
  Clone variation with respect to NM_004426.2
  93 t=>a;918 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004426 unedited
  TATAGGCGGCCGCGAATTCGGCACGAGGCTCGTGTCTCTGAGTCCAGTGAACTAAATCCA
  GTAATCTATAAAACTGTGCCGTGCTGCCTCTAATCCTTACCCACTTTTTTTCTTCACATG
  CTGATAATGAGGATTCATGGGTCTTGAGTCAGACAGAGCACCAGCCTTGGGGACCCTGGA
  CCACTATCATGGAGACTGAGAGCGAGCAGAACTCCAATTCCACCAATGGGAGTTCTAGCT
  CAAGGGGCAGCTCTCGGCCCCAGATAGCTCAAATGTCACTATATGAACGACAAGCAGTGC
  AGGCTCTGCAAGCACTGCAGCGGCAGCCCAATGCAGCTCAGTATTTCCACCAGTTCATGC
  TCCAGCAGCAGCTCAGTAATGCCCAGCTGCATAGCCTGGCTGCCGTCCAGCAGGCCACAA
  TTGCTGCCAGTCGGCAGGCCAGCTCCCCAAACACCAGCACTACACAGCAGCAGACTACCA
  CCACCCAGGCCTCGATCAATCTGGCCACCACATCGGCCGCCCAGCTCATCAGCCGATCCC
  AGAGTGTGAGCTCTCCCAGTGCTACCACCTTGACCCAATCTGTGCTACTGGGGAACACCA
  CCTCCCCACCCCTCAACCAGTCTCAGGCCCAGATGTATCTACGGCCACAGCTGGGANACC
  TATTGCAGGTAAACCGAACCCTGGGTCGGAATGTGCCTCTAGCCTNCCAACTCATNCTGA
  TGCCTATGGGGGCGGTGGCTGCAGTCCAGCAGGAGGTGCCATCTGCTCAGTCTCCTGGAG
  TCATGCAATGCAGNACAGGTTCAGAACTTGCAGTAGGGAATCACAGGCCTCAGCTCAGGG
  ACCTCANATGCAGGCTCACTCAAAAGGCATTNNCTCAGAGCTNCCTGCTTGAANNCCTTC
  CAGGCTNTAGCAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004426 unedited
  NNTTACTTGNCCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTTTTACTGA
  GGAAAAAAAATTTAATATTTTTGTTATATAAGGAATAGTGCCTAAGGCAGGTACTTATAC
  TCCTGACCTCAGCCCCACACACTCTGGTTTTATAAAGCTATAGGACAGAGCAGAGATGGA
  ACTGAAAAACAGGGTAGAAAATAACATAAATTGGAGGGGAACAGTGGGATGCAGAAAGAA
  TGACAACAGCCACATGTGCCCCAGTCAAATACTTTTAGTCCCTGCAGCAGAAGATGCCAA
  CCAAGTCTCTATACTGGCTGGGGATCCTGCCATGGATGCAGGAGAAAAAATTCAATCACA
  AGGGAGTAAGTGACAACAAAAAGAAAAAACTCTGAGCCACAGCTACACTGGGAGGGGGGA
  GGACCAGATTTTGCTACTTCTGTCACACAAGCTAGAAAAACCCTACCTCTCCCATTGGGC
  AGGCAACCTGACATTCCACCCAGTTCCTTGCCTTACCATACTATGTATTCCTCCTCTTGG
  AGAATTAAGACCTGGGCTAAATGCTCCACTCCAGGATAAGGCTGAGCAGTCCTAGTTCTC
  TAGACTGCCTCTTTTATAAAGATTGGATTAAGGTACGTGAAATGGTGAGAACTGTAAAGA
  TTTTAAAAAATAAAGTAGAACCCAGAGAAAATGTCAAAGCTGCCGCCATGTAGCACCAGC
  AACCAATTCTTGCACTTCTCTTCCCTGTCTCAGTAATCCCCTACAGAAGGTTACATGATT
  GGGACAACTCTTNTCTTCCCTGCAAGTCTGCTGGTACCCAGTTATACCTGGACAGGGGAN
  AGTGTCTGCCTTAGCTGGTNTGTGCAAGAGGCCCACCCTAGTCTNNCTGAGACATTNNAT
  CTGGCGCAGATCTTGAGGCAGGNCCCAGCTGATGTCATGGACTCATAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004426
• Insert Size: 4100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004426.2, NP_004417.2
• RefSeq Size: 5206 bp
• RefSeq ORF: 3015 bp
• Locus ID: 1911
• Cytogenetics: 12p13.31
• Domains: SAM
• Protein Families: ES Cell Differentiation/IPS, Stem cell - Pluripotency
• Gene Summary: 'This gene is a homolog of the Drosophila polyhomeotic gene, which is a member of the Polycomb group of genes. The gene product is a component of a multimeric protein complex that contains EDR2 and the vertebrate Polycomb protein BMH1. The gene product, the EDR2 protein, and the Drosophila polyhomeotic protein share 2 highly conserved domains, named homology domains I and II. These domains are involved in protein-protein interactions and may mediate heterodimerization of the protein encoded by this gene and the EDR2 protein. [provided by RefSeq, Jul 2008]'