ID4 (NM_001546) Human Untagged Clone

CAT#: SC127564

ID4 (untagged)-Human inhibitor of DNA binding 4, dominant negative helix-loop-helix protein (ID4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ID4
• Synonyms: bHLHb27; IDB4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127564 sequence for NM_001546 edited (data generated by NextGen Sequencing)
  ATGAAGGCGGTGAGCCCGGTGCGCCCNTCNNGCCGCAAGGCGCCGTCGGGCTGCGGCGGC
  GGGGAGCTGGCGCTGCGCTGCCTGGCCGAGCACGGCCACAGCCTGGGTGGCTCCGCAGCC
  GCGGCGGCGGCGGCGGCGGCAGCGCGCTGTAAGGCGGCCGAGGCGGCGGCCGACGAGCCG
  GCGCTGTGCCTGCAGTGCGATATGAACGACTGCTATAGCCGCCTGCGGAGGCTGGTGCCC
  ACCATCCCGCCCAACAAGAAAGTCAGCAAAGTGGAGATCCTGCAGCACGTTATCGACTAC
  ATCCTGGACCTGCAGCTGGCGCTGGAGACGCACCCGGCCCTGCTGAGGCAGCCACCACCG
  CCCGCGCCGCCACACCACCCGGCCGGGACCTGTCCAGCCGCGCCGCCGCGGACCCCGCTC
  ACTGCGCTCAACACCGACCCGGCCGGCGCGGTGAACAAGCAGGGCGACAGCATTCTGTGC
  CGCTGA
  
  Clone variation with respect to NM_001546.2
  27 c=>n;30 g=>n;31 g=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001546 unedited
  TTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGGTCAATTGTTGGGCTCG
  GGAGTGTCGCGGTGCCCCGAGCGCGCCGGGCGCGGAGGCAAAGGGAGCGGAGCCGGCCGC
  GGACGGGGCCCGGAGCTTGCCTGCCTCCCTCGCTCGCCCCAGCGGGTTCGCTCGCGTAGA
  GCGCAGGGCGCGCGCGATGAAGGCGGTGAGCCCGGTGCGCCCCTCGGGCCGCAAGGCGCC
  GTCGGGCTGCGGCGGCGGGGAGCTGGCGCTGCGCTGCCTGGCCGAGCACGGCCACAGCCT
  GGGTGGCTCCGCAGCCGCGGCGGCGGCGGCGGCGGCAGCGCGCTGTAAGGCGGCCGAGGC
  GGCGGCCGACGAGCCGGCGCTGTGCCTGCAGTGCGATATGAACGACTGCTATAGCCGCCT
  GCGGAGGCTGGTGCCCACCATCCCGCCCAACAAGAAAGTCAGCAAAGTGGAGATCCTGCA
  GCACGTTATCGACTACATCCTGGACCTGCAGCTGGCGCTGGAGACGCACCCGGCCCTGCT
  GAGGCAGCCACCACCGCCCGCGCCGNCACACCACCCGGCCGGGACCTGTCCAGCCGCGCC
  GCCGCGGACCCCGCTCACTGCGCTCAACACCGACCCGGCCGGGCGCGGTGAACAAGCAGG
  GCGACAGCATTCTGTGCCGCTGAGCCGCGCTGTCCANGTGTGCGGNCGCCTGAGCCCGAG
  CCAGGAGCACTAGAGAGGGGAGGGGGAGAGCAGNAGTTANAGAAAAAAAGCCACCGGAGG
  GAAAGAAAAAACATCGGCCAACCTAGAAACGTTTTCATTCGTCATTCCCAGAGAGAGAGA
  GGGAAAGAAAATTCCACTTTCATTCTTTCTTTGCACGTTCATAAACATTCTACATACGTA
  TTCTCCTTTTGGCCTCTCCATT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001546
• Insert Size: 3100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001546.2, NP_001537.1
• RefSeq Size: 2389 bp
• RefSeq ORF: 486 bp
• Locus ID: 3400
• Cytogenetics: 6p22.3
• Domains: HLH
• Protein Families: ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: TGF-beta signaling pathway
• Gene Summary: 'This gene encodes a member of the inhibitor of DNA binding (ID) protein family. The encoded protein lacks DNA binding ability, and instead regulates gene expression through binding to and inhibiting basic helix-loop-helix transcription factors. This protein has been implicated in the regulation of diverse cellular processes that play a role in development and tumorigenesis. [provided by RefSeq, Aug 2017]'