gamma Adducin (ADD3) (NM_019903) Human Untagged Clone

CAT#: SC127707

ADD3 (untagged)-Human adducin 3 (gamma) (ADD3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ADD3
• Synonyms: ADDL; CPSQ3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_019903, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGCTCAGATGCCAGCCAAGGCGTGATTACCACTCCTCCTCCTCCCAGCATGCCTCACAAAGAGAGAT
  ATTTTGACCGCATCAATGAAAATGACCCAGAATACATTAGGGAGAGGAACATGTCTCCTGATCTACGACA
  AGACTTCAACATGATGGAGCAGAGGAAACGAGTTACTCAGATCCTGCAAAGTCCTGCCTTTCGGGAAGAC
  TTGGAATGCCTTATTCAAGAACAGATGAAGAAAGGCCACAACCCAACTGGATTACTAGCATTACAGCAGA
  TTGCAGATTACATCATGGCCAATTCTTTCTCGGGTTTTTCTTCACCTCCTCTCAGTCTTGGCATGGTCAC
  ACCTATCAATGACCTTCCTGGTGCAGATACATCCTCATATGTGAAGGGAGAAAAACTTACTCGCTGTAAA
  CTTGCCAGCCTGTACAGACTTGTAGACTTGTTTGGATGGGCACACCTGGCAAATACCTATATCTCAGTAA
  GAATAAGTAAGGAGCAAGACCACATTATAATAATTCCCAGAGGCCTATCTTTTTCTGAAGCTACAGCCTC
  CAATTTGGTGAAAGTCAATATAATAGGAGAAGTGGTTGACCAGGGAAGTACCAATTTGAAAATTGACCAT
  ACAGGATTCAGTCCCCATGCTGCAATCTATTCAACACGTCCTGATGTTAAGTGTGTGATACACATCCATA
  CCCTTGCAACAGCAGCTGTATCCTCCATGAAATGTGGGATCCTTCCAATTTCTCAAGAGTCTCTTCTTCT
  GGGAGATGTTGCCTATTATGACTACCAAGGGTCACTTGAAGAACAGGAGGAGAGAATTCAACTGCAGAAG
  GTTCTGGGACCAAGTTGTAAGGTGCTGGTACTCAGGAATCATGGTGTGGTTGCACTTGGAGAAACATTAG
  AGGAGGCTTTTCATTATATTTTTAATGTGCAACTAGCCTGTGAGATTCAGGTGCAGGCCCTAGCAGGTGC
  AGGTGGAGTAGACAATCTCCATGTACTGGACTTTCAGAAGTATAAAGCTTTCACTTACACTGTAGCAGCG
  TCTGGTGGAGGAGGTGTGAATATGGGTTCCCATCAAAAATGGAAGGTTGGCGAAATTGAGTTTGAAGGGC
  TTATGAGGACTCTGGACAACTTGGGGTATAGAACAGGCTATGCTTACAGGCATCCTCTCATTCGAGAGAA
  GCCTAGGCACAAGAGTGATGTGGAAATCCCAGCAACTGTGACTGCTTTTTCCTTTGAAGACGATACAGTG
  CCACTCTCTCCTCTCAAATACATGGCACAGAGGCAACAGCGTGAAAAAACAAGATGGCTGAACTCACCAA
  ATACTTACATGAAAGTGAATGTGCCTGAGGAGTCTCGGAACGGAGAAACCAGTCCCCGAACCAAAATCAC
  GTGGATGAAAGCAGAAGACTCATCTAAAGTTAGTGGTGGAACACCTATCAAAATTGAAGATCCAAATCAG
  TTTGTTCCTTTAAACACAAACCCGAATGAGGTACTAGAAAAGAGAAATAAGATTCGGGAACAAAATCGAT
  ATGACTTGAAAACAGCAGGACCACAATCTCAGTTGCTTGCTGGAATTGTTGTGGATAAGCCACCTTCTAC
  TATGCAATTTGAAGATGATGATCATGGCCCACCAGCTCCTCCTAACCCATTTAGTCATCTCACAGAAGGA
  GAACTTGAAGAGTATAAGAGGACAATCGAACGTAAACAACAAGGCCTAGAAGAAAACCATGAGCTGTTTT
  CCAAGAGCTTCATCTCCATGGAAGTGCCTGTCATGGTAGTAAATGGCAAGGATGATATGCATGATGTTGA
  AGATGAGCTTGCTAAGCGAGTGAGTAGGTTAAGCACAAGTACAACCATAGAAAACATCGAGATTACTATT
  AAGTCTCCAGAGAAAATCGAAGAAGTCCTGTCACCTGAAGGCTCCCCTTCAAAATCGCCATCCAAGAAAA
  AGAAGAAATTCCGCACTCCTTCTTTTCTGAAAAAGAACAAAAAAAAGGAGAAAGTTGAGGCCTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_019903 unedited
  TTTTGTAATACGACTCACTATAGGGCGGCCCGCGAATTCGCACGAGGCCCGTAACGGTCG
  CCAGTGTGAGGGGNNCGGGAGGGAAAGAAGAGGGGTTTAAATTAGATTTTTTAAAACACA
  GAGCAAGCGCCAGAGGCGTCGGCATCCCAGGTGTCGCCGCTTCCTGCTGCACAGGGCTCG
  GCGTACAGGTCCCTCCCTCCTCAAGCCCCCTCCCCTTCTCCCGCCCTACCCTCTGGGGCT
  CTGCGGCGCTTAAGAGGCGGCCGCAGCGGCGGATCCGGCGGCTGCTGCAGCCCGGGCGGC
  TGCCGAGAAGGAGGGAGGGGAAACACAAAGCCGGCTACGCGCTGCGAGATAACAAGAGTA
  ATCCACAGACTTAAAACATGAGCTCAGATGCCAGCCAAGGCGTGATTACCACTCCTCCTC
  CTCCCAGCATGCCTCACAAAGAGAGATATTTTGACCGCATCAATGAAAATGACCCAGAAT
  ACATTAGGGAGAGGAACATGTCTCCTGATCTACGACAAGACTTCAACATGATGGAGCAGA
  GGAAACGAGTTACTCAGATCCTGCAAAGTCCTGCCTTTCGGGAAGACTTGGAATGCCTTA
  TTCAAGAACAGATGAAGAAAGGCCACAACCCAACTGGATTACTAGCATTACAGCAGATTG
  CAGATTACATCATGGCCAATTCTTTCTCGGGTTTTTCTTCACCTCCTCTCAGTCTTGGCA
  TGGTCACACCTATCAATGACCTTNCTGGTGCAGATACATCCTCATATGTGAAGGGAGAAA
  AAACTACTCGCTGTANACTTGCCAGCCTGTACAGACTNGTAGACCTGTTGGNATGGGCCA
  CCTGNNCAAACCTATATCTCAGTAGAAATAAGTAGGAGCCAGACCACATTATATANTCCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_019903 unedited
  NTTACTCTGTTAACTAAACCCCTATGTTTGTGTACAAAATCCCTTTGTTGAAAATAAGGG
  CTTTCTAAACTAATAAAAAAGGAGTTTTCAAAATTATAGTTTATTAAAACAACTTTTTTG
  GCAAACAAAGTTACTTCAGGTGAGGAAATTTTATACTGTGAATAAAATTCCAAATGAATC
  TTTTCTTAAAACTTTTTAAAAAATTATGTGCCAGTGTATACTAATGCTATAGATTCTTGT
  CTTAGAAGTTTTTAAAGCATTCTGTTAATGCCCACTGAAACAATGGGACTCCAAAAATAT
  AGTCAATAATCATGATAAAAAATTATAATATGATTATCAAGTGAAGCAGGTATTGAGAAA
  TAAAAATTCTCACTTGCTCACTGGCAATTTCTTTCTAACAGATATTATGGAGAAGGCCTG
  AAGTAATTCAGACAGATAGCTGTTTATGGTGAATTATAATAATACTTCATGAGGGCAGAG
  CTAATTAAAACTAGTAATTGCTTAAAATTCAAAGCCATTTCTGGACATATAAAATGAGAG
  ATGAATCTGAAAGTTTTTCTTTTGTAAAACCTCTTTCCAGTTCTTAAAGTCCAGTTTGCT
  ACATCCCCCAATCTGATCTACCATTGCATATTAATGATCAACTTAAATGTGGTATTTAGA
  TGTGCAATGTCACATTGTTATAATAATAATTATAAAAAGACTTTATTTAGGCCTCAACTT
  TCTCCTTTTTTTTGTTCTTTTTCAGAAAAGAGGAGTGCGGAATTTCTTCTTTTTCTTGGA
  TGGCGATTTTGAAAGGGAGCCTTCAGGTGACAGGACTTCTTCGATTTTCTCTGGAGACTT
  ATAGTAATCTCGATGTTTTCTATGGTTGTACTTGTGCTTAACCTACTCACTCCCTTACAA
  GCTCATCTTCACA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_019903
• Insert Size: 3000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019903.3, NP_063968.1
• RefSeq Size: 4195 bp
• RefSeq ORF: 2025 bp
• Locus ID: 120
• Cytogenetics: 10q25.1-q25.2
• Domains: Aldolase_II
• Gene Summary: 'Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced adducin gamma transcripts encoding different isoforms have been described. The functions of the different isoforms are not known. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate in-frame exon in the 3' coding region compared to variant 1.The encoded isoform (b) is shorter than isoform a. Both variants 2 and 3 encode the same isoform (b).