Guanylate kinase (GUK1) (AL832986) Human Untagged Clone

CAT#: SC127790

(untagged)-Human mRNA, cDNA DKFZp666D023 (from clone DKFZp666D023)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GUK1
• Synonyms: GMK
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AL832986, the custom clone sequence may differ by one or more nucleotides
  
  
  CGGACGCGTGGGGCGGACGCGTGGGATCACAGATAGGAGTCCCAAGTCCTATGAGAAGTTCCTGGAATAG
  GTGTAGATTCAGTAGATCTTTACAAGACCATATCTGCAGGGCAAGGTACCAGAGGACAGAGGCGGGGACA
  GGGACACTTCCATTCCAGACCTAGCAGCCCAGCACTCAGCACCATGCATGGGAGCAAATGGCTGGACTCC
  TGGGTGGGGTGGGGGTCTCAGAGCAGGCTCCCAGAGGGCTTGGAGGTGACTCCACCAGGTGGGGACGGCA
  GCTCCCAGGTAGGGTGTCATCAGAGTAGACAGCATTGCTTGCTAGGGACCCCTGGGGAGGCTGACAGGGT
  CAGTGGGTTTCAGTTGGGGGGCTCCCCTGCTGAGAACCCAGTAAAGCCGGCCTTCCATTCGTCTCCCGTG
  TGCCCAGAGCCTGGTCTGAGGGCCGCCCTGTGCATGCCGGCCCTTCCAACGTGGCAGAGCTCAGGGGGAA
  GAACACCCAGGCTCTCAGGAGACTCTCAGGCCAATGTCTCCATCCCTGGGTCAGCCCTTTCCTGCCATGA
  ATTCAGGAAGGCAGAGGCAGCTCAGCAGATGGGGACTAGAGGCCGCACTGCTATCCACAGCCTCTCTTCT
  CACCCCCAGGCATGTCGGGCCCCAGGCCTGTGGTGCTGAGCGGGCCTTCGGGAGCTGGGAAGAGCACCCT
  GCTGAAGAGGCTGCTCCAGGAGCACAGCGGCATCTTTGGCTTCAGCGTGTCCCGTGAGTCCAGGGCTCTC
  GTGGAGGGGTGCGTAGACCTCAAGGCTGCTGAGTAGTCCTAGCACCGTGAGCAGGCCAGGAGCCCAAACC
  CAACAGGCACACCCACCCTGCAGACTGTCCGAACTCTTGCACACTCCCCCCCACACAGAACCTGAGGTTA
  TCACACTCCTGCTGTCCTGCGTGCCTGTGTCTCCCTTCCCTGGGTCTGTTGAGTACTGATAACTGGGCCA
  CAGTGTTTCTTTCTGGGAGAACCCTCGCCTTGTAGGCTCCTGCGCCTTCCCAGTGGTGTGCTTCACTGGC
  TGCCTGCATCCTGGGGCTCAAGTGCTGTCGGGACTGCAAGGGAAACGCTGGGTGGGGCATTGGGCTCCGA
  GCAGCCCCCGATGGGTGACAGGTCTCTCTGCTAGATACCACGAGGAACCCGAGGCCCGGCGAGGAGAACG
  GCAAAGGTGAGTGGGGTGGGGCCCTATGGCTGGAGCACCCCCAGTGTGGGCAGGGCTGCTGGGCCCTGCA
  GCTGTGTTGGCTGTGCTGCCCGTCTCCTGCCCCCATCAATCCCTAATCTGTGAGATGGGTCCTTGCCTCC
  AAGGGCCGGTGAACTCAATCAGGGTGTCAGCGCCACAGCGTGGTGTCGCCTTCCTTGGGTACAGTGTGAG
  AGGCCGGCCAAGGCCTGGGGCTGTCTTCCTCCCACCTTGGAGGCGGCCACAGTGCTGCTGTCCCCAGCCC
  TGTCCTGGACTCGGCACTTATCAGCACTTTTGAGCTGTCTTCTGGGGTCCTGGTAAAAAGGGCTACTCTG
  CCTGCCTGATTCAAGACAAGGGACCCCCTTCCCAACAGCACCCCCGCCCCTTGCCGTGCAACCCAGTGGT
  CTCCAGTCACCCCACCACATCGTCCCCTCTGTAACCTGACGGTCTCCAGTTCCCCCACCACCTTCCCCCA
  GAACCTGTTGGTCTCCAGTCCCCACCCCATCACCACCAACTCCCAACTCCCCACTGGAACCCAGCAGTCT
  CGTATCTCCATCAGTGAGGACAGTGTGAGAAATGGTGTCTGGCTCAGGCACTTGGCAGCACTTGAGGGGT
  CCTCAGATGTCTCCTGCCCAGCAAGGCTGACTAAAGCAGTCATGGGTGTGGGAGGGGCCTGCAGGCATGC
  CTGGGTTGGGGGCAGCTGGCCCTGGGCACCCTGGTGCAGGTCCAGTCTGCCCTCTGGATGGCCCCTCCTC
  TTCCCCAGATTACTACTTTGTAACCAGGGAGGTGATGCAGCGTGACATAGCAGCCGGCGACTTCATCGAG
  CATGCCGAGTTCTCGGGGAACCTGTATGGCACGAGCAAGGTGGCGGTGCAGGCCGTGCAGGCCATGAACC
  GCATCTGTGTGCTGGACGTGGACCTGCAGGGTGTGCGGAACATCAAGGCCACCGATCTGCGGCCCATCTA
  CATCTCTGTGCAGCCGCCTTCACTGCACGTGCTGGAGCAGCGGCTGCGGCAGCGCAACACTGAAACCGAG
  GAGAGCCTGGTGAAGCGGCTGGCTGCTGCCCAGGCCGACATGGAGAGCAGAAATCAAGAAAGTTCAAAGG
  ACCGGCGCCTGAGGCTTGCTGTCTGTTCTCGGCACCCCGGGCCCATACAGGACCAGGGCAGCAGCATTGA
  GCCACCCCCTTGGCAGGCGATACGGCAGCTCTGTGCCCTTGGCCAGCATGTGGAGTGGAGGAGATGCTGC
  CCCTGTGGTTGGAACATCCTGGGGTGACCCCCGACCCAGCCTCGCTGGGCTGTCCCCTGTCCCTATCTCT
  CACTCTGGACCCAGGGCTGACATCCTAATAAAATAACTGTTGGATTAGAAACTCCAAAAAAAAAAAAAAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AL832986 unedited
  GGATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAGGTGGCCCC
  GGATGCTGCGGCGCCCGCTGGCCGGGCTGGCTGCGGCCGCCCTGGGCCGGGCCCCACCGG
  ACGGCATGTCGGGCCCCAGGCCTGTGGTGCTGAGCGGGCCTTCGGGAGCTGGGAAGAGCA
  CCCTGCTGAAGAGGCTGCTCCAGGAGCACAGCGGCATCTTTGGCTTCAGCGTGTCCCGTG
  AGTCCAGGGCTCTCGTGGAGGGGTGCGTAGACCTCAAGGCTGCTGAGTAGTCCTAGCACC
  GTGAGCAGGCCAGGAGCCCAAACCCAACAGGCACACCCACCCTGCAGACTGTCCGAACTC
  TTGCACACTCCCCCCCACACAGAACCTGAGGTTATCACACTCCTGCTGTCCTGCGTGCCT
  GTGTCTCCCTTCCCTGGGTCTGTTGAGTACTGATAACTGGGCCACAGTGTTTCTTTCTGG
  GAGAACCCTCGCCTTGTAGGCTCCTGCGCCTTCCCAGTGGTGTGCTTCACTGGCTGCCTG
  CATCCTGGGGCTCAAGTGCTGTCGGGACTGCAAGGGAAACGCTGGGTGGGGCATTGGGCT
  CCGAGCAGCCCCCGATGGGTGACAGGTCTCTCTGCTAGATACCACGAGGAACCCGAGGCC
  CGGCGAGGAGAACGGCANAGGTGAGTGGGGTGGGGCCCTATGGCTGGAGCACCCCCAGTG
  TGGGCAGGGCTGCTGGGCCCTGCAGCCTGTGTGGCTGTGCTGCCCGTCTCCTGCCCCCAT
  AATCCNTAATCTGTGAGATGGGTCCTTGCCTCCAGGGCCCGTGAACTCCATCAGGGTGTC
  AGCGCCCCAGCGTGGTGTCGCCTTCCTTGGGTACAGTGTGAGAAGGCCGCCAGGCCTGGN
  GCTGTNCTCCTCCCACCTGNNAGNGNCACAGTGCTGCTGTCCCCAGCCTGTCCTGGAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for AL832986 unedited
  GCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTCTAATCCAACAGTTATTTTATTAGGA
  TGACAACCCTGGGTCCAGAGCGAGAGATAGGGACAGGGGACACCCCACCGAGGCTGGGTC
  GGGGGTCACCCCAGGATGTTCTAACCATAGGGGCAGCATATTCTTCACTTCACATGCTGC
  CCAAGGGCACTCCACCTGACGTCATCATCTCCCAAGGGGCCGCCCTCAATGCTCCTGCAT
  TGAATCTGAATGGCCCTGGTGTGTCCCCACCCTCACCTCCCCCACTCGTTACATATCTCT
  TACTTCCCGTCCACTCCCTTCATAATCCCCCTTCCCTCCCCCCCCTCCCCCCCACTTCCC
  CTTCCTCTCTTTATCATTTTTTTACCCCGTCCTCCCTACCCTTTTTTTCCTACTCCCTCC
  TTTGGTCTTACTCCCATTATTCCACCCCCCTCTCCCCTATCCCCTCTTCTCTACCCAATC
  CGACCCCCCTATTTCCCACCACCCTATAAATACCCCCCTTCCCCTTTTCTATACCACCAC
  CTCCCCTTTCTGCCACTTATTCTTTCCTCAACTCTCCCCCTCCCCATTCCCATCCTTCTT
  CCTTACCCACCTCTTTGCCCACACCACTCCCGAATTACATCCTCCTCCCCTTTCTTTTTT
  CCCCCCCCTCCTTCCGCGCCGTCCTACCCATCATTCTACACCCTGGACACCCTACTTTCC
  CTCACCATTTTTATCCCCTTCTACCAACCACCCGCCCATTTCCCGCCCATACCATCATCT
  TACCCAGCCCCAATTCCCCCACAAAACACTTCCTCCTTCCCCATTTCCCATTTTATCCTC
  CTCACTCTTCCACCCTTCCTCTACTACCTTTTTCCACCCCCCCCCCTCTTTTCCTCCCCT
  CCTCTAATCTATATGTTCTACTATTCTACCCACCCCTCACTCCCACCCCCGATACCCCGT
  CNTACTATACCCCCTTCCCCAATTCTTTTCCTTCCCCCCTTTTCTCTTCCCATTTCCCCT
  CCT
  
• Restriction Sites: NotI-NotI
• ACCN: AL832986
• Insert Size: 2200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AL832986.1, CAH56207.1
• RefSeq Size: 2590 bp
• RefSeq ORF: 2590 bp
• Locus ID: 2987
• Cytogenetics: 1q42.13
• Domains: Guanylate_kin, GuKc
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Purine metabolism
• Gene Summary: 'The protein encoded by this gene is an enzyme that catalyzes the transfer of a phosphate group from ATP to guanosine monophosphate (GMP) to form guanosine diphosphate (GDP). The encoded protein is thought to be a good target for cancer chemotherapy. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2011]'