SESN1 (NM_014454) Human Untagged Clone

CAT#: SC127795

SESN1 (untagged)-Human sestrin 1 (SESN1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SESN1
• Synonyms: PA26; SEST1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127795 sequence for NM_014454 edited (data generated by NextGen Sequencing)
  ATGGCTGAAGGAGAGAATGAAGTGAGATGGGATGGACTCTGCAGCAGAGATTCAACTACT
  AGGGAGACAGCATTGGAAAACATTAGGCAAACCATTTTGAGGAAAACCGAGTATCTTCGT
  TCGGTGAAAGAAACACCTCATCGTCCATCAGACGGGCTTTCAAATACCGAGTCTTCGGAT
  GGGTTGAATAAGCTACTTGCTCATCTGCTTATGCTTTCTAAGAGGTGTCCCTTCAAAGAT
  GTGAGAGAGAAAAGTGAGTTTATTCTGAAGAGCATCCAGGAACTTGGCATTAGAATTCCT
  CGACCACTAGGACAGGGACCAAGCAGATTCATCCCAGAAAAGGAGATCCTCCAAGTGGGG
  AGTGAAGACGCACAGATGCATGCTTTATTTGCAGATTCTTTTGCTGCTTTGGGCCGTTTG
  GATAACATTACGTTAGTGATGGTTTTCCACCCACAATATTTAGAAAGTTTCTTAAAAACT
  CAGCACTATCTACTGCAAATGGATGGGCCGTTACCCCTACATTATCGTCACTACATTGGA
  ATAATGGCTGCGGCAAGACATCAGTGCTCCTACTTAGTGAACCTGCATGTAAATGATTTC
  CTTCATGTTGGTGGGGACCCCAAGTGGCTCAATGGTTTAGAGAATGCTCCTCAAAAACTA
  CAGAATTTAGGAGAACTTAACAAAGTGTTAGCCCATAGACCTTGGCTTATTACCAAAGAA
  CACATTGAGGGACTTTTAAAAGCTGAAGAGCACAGCTGGTCCCTTGCGGAATTGGTACAT
  GCAGTAGTTTTACTCACACACTATCATTCTCTTGCCTCATTCACATTCGGCTGTGGAATC
  AGTCCAGAAATTCATTGTGATGGTGGCCACACATTCAGACCTCCTTCTGTTAGCAACTAC
  TGCATCTGTGACATTACAAATGGCAATCACAGTGTGGATGAGATGCCGGTCAACTCAGCA
  GAAAATGTTTCTGTAAGTGATTCTTTCTTTGAGGTTGAAGCCCTCATGGAAAAGATGAGG
  CAGTTACAGGAATGTCGAGATGAAGAAGAGGCAAGTCAGGAAGAGATGGCTTCACGTTTT
  GAAATAGAAAAAAGAGAGAGTATGTTTGTCTTCTCTTCAGATGATGAAGAAGTTACACCA
  GCAAGAGCTGTATCTCGTCATTTTGAGGATACTAGTTATGGCTATAAAGATTTCTCTAGA
  CATGGGATGCATGTTCCAACATTTCGTGTCCAGGACTATTGCTGGGAAGATCATGGTTAT
  TCTTTGGTAAATCGCCTTTATCCAGATGTGGGACAGTTGATTGATGAAAAATTTCACATT
  GCTTACAATCTTACTTATAATACAATGGCAATGCACAAAGATGTTGATACCTCAATGCTT
  AGACGGGCAATTTGGAACTATATTCACTGCATGTTTGGAATAAGATATGATGATTATGAC
  TATGGTGAAATTAACCAGCTATTGGATCGTAGCTTTAAAGTTTATATCAAAACTGTTGTT
  TGCACTCCTGAAAAGGTTACCAAAAGAATGTATGATAGCTTCTGGAGGCAGTTCAAGCAC
  TCTGAGAAGGTTCATGTTAATCTGCTTCTTATAGAAGCTAGGATGCAAGCAGAACTCCTT
  TATGCTCTGAGAGCCATTACCCGCTATATGACCTGA
  
  Clone variation with respect to NM_014454.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014454 unedited
  ATTTTGTATACGACTCACTATAGGCGGCCGCGAATTCGGCACGAGGGCGGGGTGGGAACG
  CCGGAGAGAACCCGGTGGCTGCACAGACAAAAAAGCCCCGAATGGCTGGAGGGCGTTCAG
  CTGTTAACAGCCTTTTGGGGCAGAGCACGGATTTGACAGCTCCACAACGTGAGGATATCC
  ACTGACCCCGCGAGACGGAGGAGAACGCTTCCCCGAAATTCTCTGCCCACCAAAGCCAGC
  GCTGCAAGGTTGCAACTTTCAAACTTTGTTTTTCCAGAAAGAAGACTGCCCTTTCGTGTA
  CAAGGAGAGGGTGAGAGGGTGACCTANCTTGTAGATCGGCTGAAGGCACCAGTGGTTCCA
  AATGTCACCCAGATGTGTGTTTTCATGACGATTTGATTTCTCTGATTTTATTTTTACATT
  TTTCATTTTAAAAATACAAAGCAATTTTTTTGGGGCATGCTGAAAGGTAACTGAAGACCG
  CAAAGGAAAAACTATTGTCATGGCTGAAGGAGAGAATGAAGTGAGATGGGATGGACTCTG
  CAGCAGAGATTCAACTACTAGGGAGACAGCATTGGAAAACATTAGGCAAACCATTTTGAG
  GAAAACCGAGTATCTTCGTTCGGTGAAAGAAACACCTCATCGTCCATCAGACGGGCTTTC
  AAATACCGAGTCTTCGGATGGGTTGAAATAGCTACTTGCTCATCTGCTTTATGCTTCTAA
  AGAGTGTCCCCTTCAAAGATGTGAGAGAGAAAAGTGAGTTTATTCTGAAGAGCATCCAGG
  AACTTGGCATTAGAATTCCTCGACCACTTAGACAGGGACCCAAGCAGATCTTCCCAGAAA
  GGAGATCTNCCAGTGGGGAGTGAAGACCCCAGATGCATGCTTTATTGGCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014454 unedited
  CGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTGGAGAAAAATCTTCTTTATTTAAAA
  ATACCAGTAATACTGACAGACTTCAAAAGCAATTCACGCTTCCAGAATACAAAGTACTTA
  ATACATATTTTCAAACCTGTTTGCATTTCAAACAAAGTTAGCGTTTTTGTAAATCAAATT
  TGATAACCCGACTAAAAATATTTTCCAGCTTTATTATTTAAAGAGCTGCACAGCCTTTAA
  AGTGGGGACCAGGAGGCAGGCAGAGGCAGATAGACTGAATGCACCCAGGACTGCGCAGCA
  GTCTACAGCAACATGTCCCACAACTTTGGTGCTGGAAACACAAGTAATGCACAAGACAGC
  TGCCCTCCAGTGTCAGGATCCTGTGAAACAGCATATCAAAAGATCGCCAGCTTCTTATAA
  TTTACACACTTTCATTTAAGATTGCTTTTTGAAGAAAATCTTTAAGAATGCCATTTTAAT
  TTAATATCCAGAACCCTGAATTTAAAAAACTAATGAAAGAAATTAACTGTTACCATCAAA
  ATGGTTGTTGCTGAGCTGTGTGAGTAAGAATTCTATGCTTTTTTAGATGCAAGAAAATTA
  ACACATAATGCAAGTTTAATTTGAAATCACACAAGTTATGTATGTAAGCTCAGATAGTAG
  CAATGTTCACTTTTATTACTAATAGCTTAACTGAAACAGCCATCTATTCATTCCTNCAGC
  AGGAATAGCAGCACCAAGATCTTCTAAAATCCTTTACATATAAAAGCTAAGTCATTTATC
  CAGTCCCATTCCTTTGCATGCTCCAATTCTTGTTATCTATTTAACATAAAAGCACAGGAT
  TTGGAGAGTTACTATTTGCTGTTTAAAACAGTTCCCAGCATTTGGACTGTCAAAAGCAAA
  ACTGGGATGGCACCCGNCTTCAGTCTAACCTGAACCGCCAAAGCACTGCTGGGCCACAAG
  TGNTTCACGAAAATTCCGATCTAAGATTTGCCCATGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014454
• ORF Size: 1656 bp
• Insert Size: 3090
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_014454.1, NP_055269.1
• RefSeq Size: 2785
• RefSeq ORF: 1656
• Locus ID: 27244
• Domains: PA26
• Protein Pathways: p53 signaling pathway
• Gene Summary: This gene encodes a member of the sestrin family. Sestrins are induced by the p53 tumor suppressor protein and play a role in the cellular response to DNA damage and oxidative stress. The encoded protein mediates p53 inhibition of cell growth by activating AMP-activated protein kinase, which results in the inhibition of the mammalian target of rapamycin protein. The encoded protein also plays a critical role in antioxidant defense by regenerating overoxidized peroxiredoxins, and the expression of this gene is a potential marker for exposure to radiation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.