PPAR delta (PPARD) (NM_006238) Human Untagged Clone

CAT#: SC127818

PPARD (untagged)-Human peroxisome proliferator-activated receptor delta (PPARD), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPARD
• Synonyms: FAAR; NR1C2; NUC1; NUCI; NUCII; PPARB
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_006238 edited
  ATGGAGCAGCCACAGGAGGAAGCCCCTGAGGTCCGGGAAGAGGAGGAGAAAGAGGAAGTG
  GCAGAGGCAGAAGGAGCCCCAGAGCTCAATGGGGGACCACAGCATGCACTTCCTTCCAGC
  AGCTACACAGACCTCTCCCGGAGCTCCTCGCCACCCTCACTGCTGGACCAACTGCAGATG
  GGCTGTGACGGGGCCTCATGCGGCAGCCTCAACATGGAGTGCCGGGTGTGCGGGGACAAG
  GCATCGGGCTTCCACTACGGTGTTCATGCATGTGAGGGGTGCAAGGGCTTCTTCCGTCGT
  ACGATCCGCATGAAGCTGGAGTACGAGAAGTGTGAGCGCAGCTGCAAGATTCAGAAGAAG
  AACCGCAACAAGTGCCAGTACTGCCGCTTCCAGAAGTGCCTGGCACTGGGCATGTCACAC
  AACGCTATCCGTTTTGGTCGGATGCCGGAGGCTGAGAAGAGGAAGCTGGTGGCAGGGCTG
  ACTGCAAACGAGGGGAGCCAGTACAACCCACAGGTGGCCGACCTGAAGGCCTTCTCCAAG
  CACATCTACAATGCCTACCTGAAAAACTTCAACATGACCAAAAAGAAGGCCCGCAGCATC
  CTCACCGGCAAAGCCAGCCACACGGCGCCCTTTGTGATCCACGACATCGAGACATTGTGG
  CAGGCAGAGAAGGGGCTGGTGTGGAAGCAGTTGGTGAATGGCCTGCCTCCCTACAAGGAG
  ATCAGCGTGCACGTCTTCTACCGCTGCCAGTGCACCACAGTGGAGACCGTGCGGGAGCTC
  ACTGAGTTCGCCAAGAGCATCCCCAGCTTCAGCAGCCTCTTCCTCAACGACCAGGTTACC
  CTTCTCAAGTATGGCGTGCACGAGGCCATCTTCGCCATGCTGGCCTCTATCGTCAACAAG
  GACGGGCTGCTGGTAGCCAACGGCAGTGGCTTTGTCACCCGTGAGTTCCTGCGCAGCCTC
  CGCAAACCCTTCAGTGATATCATTGAGCCTAAGTTTGAATTTGCTGTCAAGTTCAACGCC
  CTGGAACTTGATGACAGTGACCTGGCCCTATTCATTGCGGCCATCATTCTGTGTGGAGAC
  CGGCCAGGCCTCATGAACGTTCCACGGGTGGAGGCTATCCAGGACACCATCCTGCGTGCC
  CTCGAATTCCACCTGCAGGCCAACCACCCTGATGCCCAGTACCTCTTCCCCAAGCTGCTG
  CAGAAGATGGCTGACCTGCGGCAACTGGTCACCGAGCACGCCCAGATGATGCAGCGGATC
  AAGAAGACCGAAACCGAGACCTCGCTGCACCCTCTGCTCCAGGAGATCTACAAGGACATG
  TACTAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006238 unedited
  TTGTTCCCCCGCCCGTTGCCGCATATGGGCGGTAGCGTGTACGGTGGGAGGTCTATATAA
  GCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCG
  CGAATTCGGCACGAGGGAATTCTGCGGAGCCTGCGGGACGGCGGCGGTGGCGCCGTATGC
  AGCCGGGACAGTGTTGTACAGTGTTTTGGGCATGCACGTGATACTCACACAGTGGCTTCT
  GCTCACCAACAGATGAAGACAGATGCACCAACGAGGAGGTGAGGAGCGCCTCTTTCCGGC
  CGCCATCACATCTAGGAAGGAGGAGCGTCTCTGCCCGGCCGCCCATCGTCTGAGATGTGG
  GGAGCGCCTCTGCCCCGCCGCCCCGTCTGGGATGTGAGGAGCACCTCTGCCCGGCCACGA
  CCCCGTCTGGGAGGTGAGGAGCATCTCTGCCCCGCCGCCCCGTCTGAGAAGTGAGGAGAC
  CCTCTGCCCGGCAACCACCCCGTCTGAGAAGTGAGGAGACCCTCCACCCGGCAGCTGCCC
  CGTCTGAGAAATGAGGAGCCTCTCCGCCCGGCAGCCACCCCGTCTGTGAAGCTGATGGGA
  ACCACCCTGTAGAGGTCCATCTGCGTTCAGACCCAGACGATGCCAGAGCTATGACTGNGC
  CTGCATGTGTGGCGCCGAGGGGAGATCATCCATGGAGCAGCCACAGGAGGAAGCCCCTGA
  GGTCCCGGAAGAGGATGAGAAAGAGGAAGTGCAGACGCAGAAGGAGCCCCAGAGCTCAAT
  GGGGGACCACAGGATGCACTTTCTTTCAGCAGCTACACAGACCTCTCCCGGAGCTCCTCG
  NCACCCTCACTGCTGGACCAACTGCAGATGGACTGTGACGGGGCCTATGCGGCAGCCTCA
  CATGGAGTGCCCGGCTGTGCCGGGACATGCATCGGACTTCACTACGGTGTTATGCATGTG
  AGGGGCAAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006238 unedited
  GGGCTCCGTTTGGTTNCNCCATTAATCTTCAACGTGNTNCGANCAGTNCTGGTGTCACAC
  TATCTTATATTACAAAACACAGGCAGTCTGGGGCTCCTAGCAAAAATATACATTTCGAAT
  TTTGGAGATTGTTCAGACACTGAGAAGAGCTGTATCCTCAGCACCAGACCCGGGTCGGGG
  CAGGGACGCGGCATGTGGTCGGTGTGGGGGAGGTGGCCTCCACCTCTGTCCCTTCCTTTT
  TTTTTTCTGCTGGTCCTCCTCCCCTCCTTCCTCCTCGGTCCGTGGTCGTCCCCGCCAATC
  GTGCCGCCCCCTCCCGCCCCGTTCCTTCCCTGGGATGCTGCACCGCCCGTCCGGCGCCGG
  GCTGGCCTCCTCTCCCCCTGGTCTTTTCTTTATGGCTCTCCGGCGCGGCGCGTCCCTGCC
  CGCCCCCCGTCTCTTGCGTTCCGCCCCCTGCTTGTCGTCCCTGTGCGCCCCCGCTCCTGC
  GTCCTTCGCCCCTGCCCCTGTTGTCGTGCATGTGGAATCCCCCGGCTTCGTCGTGCTTTT
  CTCCTTCTGCCCNCGTCTGCCCTGCGCTGCTGTCCTTCGTCCGTCTCCCGTCCCTCTCTC
  TTTCGCTCCTCCCTCTCCCGCGCCTTTTCTGTGCCCCCCCGGCCCCGCCCCGTTTCCCGC
  GTGTTCGTCGTCCCCCTCGCGCGCGTGCTCATTGCCCCTCCTCCCCGTTCCACCGGGCGC
  ATCCCCCGCGCTCTCGGTTCTCCCCGGCGCCTCCTTCGTTGCCGAGTTTTTGTCTCCCGG
  CGCGTCCTCCTCCTTGTGTTCGTGTTCGCTTCCTTTTCGTGGATTTAGTGTTTCCGACCG
  CTCGCCCCCCCTCTCGTCCCCTCCCCCTTCCTTCCCTTTCGGCGTTTCTCCCTGAACTCC
  AGCTCGTTTTTTGCTCGGCGGCGCGCTTCCTTGTGCATATNTCTCTTTCTTTTTTGCTTG
  GTCGTTCCCTCTCGCCCGCTTATTTTTCCCGTTTTCGCCTCGCCCGTTCCCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006238
• Insert Size: 4500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_006238.2.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006238.2, NP_006229.1
• RefSeq Size: 3328 bp
• RefSeq ORF: 1326 bp
• Locus ID: 5467
• Cytogenetics: 6p21.31
• Domains: HOLI, zf-C4
• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Pathways in cancer, PPAR signaling pathway, Wnt signaling pathway
• Gene Summary: 'This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) family. The encoded protein is thought to function as an integrator of transcriptional repression and nuclear receptor signaling. It may inhibit the ligand-induced transcriptional activity of peroxisome proliferator activated receptors alpha and gamma, though evidence for this effect is inconsistent. Expression of this gene in colorectal cancer cells may be variable but is typically relatively low. Knockout studies in mice suggested a role for this protein in myelination of the corpus callosum, lipid metabolism, differentiation, and epidermal cell proliferation. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms. [provided by RefSeq, Aug 2017]'
  Transcript Variant: This variant (1) encodes the longest isoform (1). Both variants 1 and 3 encode the same isoform (1).