Aromatase (CYP19A1) (NM_000103) Human Untagged Clone

CAT#: SC127848

CYP19A1 (untagged)-Human cytochrome P450, family 19, subfamily A, polypeptide 1 (CYP19A1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CYP19A1
• Synonyms: ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127848 sequence for NM_000103 edited (data generated by NextGen Sequencing)
  ATGGTTTTGGAAATGCTGAACCCGATACATTATAACATCACCAGCATCGTGCCTGAAGCC
  ATGCCTGCTGCCACCATGCCAGTCCTGCTCCTCACTGGCCTTTTTCTCTTGGTGTGGAAT
  TATGAGGGCACATCCTCAATACCAGGTCCTGGCTACTGCATGGGAATTGGACCCCTCATC
  TCCCACGGCAGATTCCTGTGGATGGGGATCGGCAGTGCCTGCAACTACTACAACCGGGTA
  TATGGAGAATTCATGCGAGTCTGGATCTCTGGAGAGGAAACACTCATTATCAGCAAGTCC
  TCAAGTATGTTCCACATAATGAAGCACAATCATTACAGCTCTCGATTCGGCAGCAAACTT
  GGGCTGCAGTGCATCGGTATGCATGAGAAAGGCATCATATTTAACAACAATCCAGAGCTC
  TGGAAAACAACTCGACCCTTCTTTATGAAAGCTCTGTCAGGCCCCGGCCTTGTTCGTATG
  GTCACAGTCTGTGCTGAATCCCTCAAAACACATCTGGACAGGTTGGAGGAGGTGACCAAT
  GAATCGGGCTATGTGGACGTGTTGACCCTTCTGCGTCGTGTCATGCTGGACACCTCTAAC
  ACGCTCTTCTTGAGGATCCCTTTGGACGAAAGTGCTATCGTGGTTAAAATCCAAGGTTAT
  TTTGATGCATGGCAAGCTCTCCTCATCAAACCAGACATCTTCTTTAAGATTTCTTGGCTA
  TACAAAAAGTATGAGAAGTCTGTCAAGGATTTGAAAGATGCCATAGAAGTTCTGATAGCA
  GAAAAAAGACGCAGGATTTCCACAGAAGAGAAACTGGAAGAATGTATGGACTTTGCCACT
  GAGTTGATTTTAGCAGAGAAACGTGGTGACCTGACAAGAGAGAATGTGAACCAGTGCATA
  TTGGAAATGCTGATCGCAGCTCCTGACACCATGTCTGTCTCTTTGTTCTTCATGCTATTT
  CTCATTGCAAAGCACCCTAATGTTGAAGAGGCAATAATAAAGGAAATCCAGACTGTTATT
  GGTGAGAGAGACATAAAGATTGATGATATACAAAAATTAAAAGTGATGGAAAACTTCATT
  TATGAGAGCATGCGGTACCAGCCTGTCGTGGACTTGGTCATGCGCAAAGCCTTAGAAGAT
  GATGTAATCGATGGCTACCCAGTGAAAAAGGGGACAAACATTATCCTGAATATTGGAAGG
  ATGCACAGACTCGAGTTTTTCCCCAAACCCAATGAATTTACTCTTGAAAATTTTGCAAAG
  AATGTTCCTTATAGGTACTTTCAGCCATTTGGCTTTGGGCCCCGTGGCTGTGCAGGAAAG
  TACATCGCCATGGTGATGATGAAAGCCATCCTCGTTACACTTCTGAGACGATTCCACGTG
  AAGACATTGCAAGGACAGTGTGTTGAGAGCATACAGAAGATACACGACTTGTCCTTGCAC
  CCAGATGAGACTAAAAACATGCTGGAAATGATCTTTACCCCAAGAAACTCAGACAGGTGT
  CTGGAACACTAG
  
  Clone variation with respect to NM_000103.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000103 unedited
  AGTTCACAATTGTATACGACNACTATAGGCGGCCGCGNAATTCGCACCAGGGCAAACAGG
  AAGGTGAAGAAGAACTTATCCTATCAGGACGGAAGGTCCTGTGCTCGGGATCTTCCAGAC
  GTCGCGACTCTAAATTGCCCCCTCTGAGGTCAAGGAACACAAGATGGTTTTGGAAATGCT
  GAACCCGATACATTATAACATCACCAGCATCGTGCCTGAAGCCATGCCTGCTGCCACCAT
  GCCAGTCCTGCTCCTCACTGGCCTTTTTCTCTTGGTGTGGAATTATGAGGGCACATCCTC
  AATACCAGGTCCTGGCTACTGCATGGGAATTGGACCCCTCATCTCCCACGGCAGATTCCT
  GTGGATGGGGATCGGCAGTGCCTGCAACTACTACAACCGGGTATATGGAGAATTCATGCG
  AGTCTGGATCTCTGGAGAGGAAACACTCATTATCAGCAAGTCCTCAAGTATGTTCCACAT
  AATGAAGCACAATCATTACAGCTCTCGATTCGGCAGCAAACTTGGGCTGCAGTGCATCGG
  TATGCATGAGATAGGCATCATATTTAACAACAATCCAGAGCTCTGGAAAACAACTCGACC
  CTTCTTTATGAAAGCTCTGTCAGGCCCCGGCCTTGTTCGTATGGTCACAGTCTGTGCTGA
  ATCCCTCANAACACATCTGGACAGGTTGGAGGATGTGACCAATGAATCGGGCTTGTGGNA
  CGTGTTGACCCTTCTGCGTCGTGTCATGCTGGACACCTCTACACGCTCTTCTTGANGATC
  CCTTTGGACGAAGTGCTATCGTGGNTAAATNCAAGGTATTNTGATGCATGGCAGCTCTCT
  CATCAACAGAATCTCTTTAGATTTCTGCTTACAAAGTTGANAAGCGTCAAGATTGAAGAT
  CCCTAAAGTCTGATACGAAAAGAGCAGATTNCCGAAAGACTGGANATGATGACTTGNCCT
  GGATGTTTACNAAAACTGTGACGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000103 unedited
  CGTTTCTTTTCCCCCCCCCCCCAATGCTGGGGAGAAGAGCCAATTGTGAGTGTTTGAATG
  ACATTCAGTAGAGATAGTTATATTGGCTATATTACACAAGTAAAGTGGTGTTTGGAAAGT
  TCCTACATTCATTTGATTTCCTTTAGAAAGCGAATTCCAAGGTGTGCAATTTATCCATGA
  CCACTTACATTGATTCATGGAACCAAAATATGTTAGATTCCCTCAGGTAACCGGTTAAAA
  TTTTGAGGTTGGAGTTAAAAAAATACTTCAGGGGGGTTTTACTTTTGGACCACCGTTTTT
  TTATTGGAGTGGGGGCCCCCTAGGATCTTTGGGGGCAGGGCGAAAAAAAAAGGGGGAAAA
  TATTTTCCGGGGGGAAAGAATAATAGGGGGTTAAAAGAGAGAGGCCCCCTCTTAAGAGAG
  TGGACACCCCCCTGGGGGGGGGGGATTTTTTTTTTCCCCAACAAACCAATCCCCTTGTTA
  TAAAGAAAACACCAAAATGGGGGCAGAAAACACGCCGGTTCCTTCTGGTGTGGCGCCCCC
  CCTCTTATTTTGGCACAACACAAAAAAGGGGGAGCACACCCCCCCCAGAGCTTCTTTTTT
  TTATTTATCTTAAAGAGGGCGCGCGGCGCGAAGATGTCTTTCCTTTCCTAAAAAAGAAAG
  AGGGCCGGCGCCAATATTAATCCCCCCCCCCCCTCTCTTAAATATATTTTTTGCCCCCGT
  GGGGGATAATATTAAAATTTGTTTCTCCCCCCATCCACACCAGCCACACCACACAAAATA
  TTGTCTCANTGTCGCCTCGGGCCGTGCGGCGCGTCTACGACCAACACCGGCGGCGGAGAA
  AACACTTCTGCCTCAATAAATTTCTCCCCCCCCCCCCCACATTTATTGTTCCACAAAACA
  AAAAGGTTTGTTTTCTTTTTTTTTAAATCGGCGCCAAGACCCGAACGTNTTTTACCACCT
  CCTAAACCACCATAAAGATCTTTTTTCGTTCCCCCCCCCCCCCCACACAAAAAAAAATTG
  TTCCTTCTCTCTCCCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000103
• Insert Size: 3050 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000103.2, NP_000094.2
• RefSeq Size: 3007 bp
• RefSeq ORF: 1512 bp
• Locus ID: 1588
• Cytogenetics: 15q21.2
• Domains: p450
• Protein Families: Druggable Genome, P450
• Protein Pathways: Androgen and estrogen metabolism, Metabolic pathways
• Gene Summary: 'This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. Alternative promoter use and alternative splicing results in multiple transcript variants that have different tissue specificities. [provided by RefSeq, Dec 2016]'
  Transcript Variant: This variant (1) represents the use of an alternate promoter and contains an alternate 5' exon (I.1), compared to variant 4. All variants encode the same protein. The 5' end of this transcript contains long terminal repeat (LTR) sequence, initiates from an LTR promoter and results in placenta-specific expression. (PMID: 19577618). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.