TNFRSF1A (NM_001065) Human Untagged Clone

CAT#: SC127913

TNFRSF1A (untagged)-Human tumor necrosis factor receptor superfamily, member 1A (TNFRSF1A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TNFRSF1A
• Synonyms: CD120a; FPF; p55; p55-R; p60; TBP1; TNF-R; TNF-R-I; TNF-R55; TNFAR; TNFR1; TNFR55; TNFR60
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127913 sequence for NM_001065 edited (data generated by NextGen Sequencing)
  ATGGGCCTCTCCACCGTGCCTGACCTGCTGCTGCCACTGGTGCTCCTGGAGCTGTTGGTG
  GGAATATACCCCTCAGGGGTTATTGGACTGGTCCCTCACCTAGGGGACAGGGAGAAGAGA
  GATAGTGTGTGTCCCCAAGGAAAATATATCCACCCTCAAAATAATTCGATTTGCTGTACC
  AAGTGCCACAAAGGAACCTACTTGTACAATGACTGTCCAGGCCCGGGGCAGGATACGGAC
  TGCAGGGAGTGTGAGAGCGGCTCCTTCACCGCTTCAGAAAACCACCTCAGACACTGCCTC
  AGCTGCTCCAAATGCCGAAAGGAAATGGGTCAGGTGGAGATCTCTTCTTGCACAGTGGAC
  CGGGACACCGTGTGTGGCTGCAGGAAGAACCAGTACCGGCATTATTGGAGTGAAAACCTT
  TTCCAGTGCTTCAATTGCAGCCTCTGCCTCAATGGGACCGTGCACCTCTCCTGCCAGGAG
  AAACAGAACACCGTGTGCACCTGCCATGCAGGTTTCTTTCTAAGAGAAAACGAGTGTGTC
  TCCTGTAGTAACTGTAAGAAAAGCCTGGAGTGCACGAAGTTGTGCCTACCCCAGATTGAG
  AATGTTAAGGGCACTGAGGACTCAGGCACCACAGTGCTGTTGCCCCTGGTCATTTTCTTT
  GGTCTTTGCCTTTTATCCCTCCTCTTCATTGGTTTAATGTATCGCTACCAACGGTGGAAG
  TCCAAGCTCTACTCCATTGTTTGTGGGAAATCGACACCTGAAAAAGAGGGGGAGCTTGAA
  GGAACTACTACTAAGCCCCTGGCCCCAAACCCAAGCTTCAGTCCCACTCCAGGCTTCACC
  CCCACCCTGGGCTTCAGTCCCGTGCCCAGTTCCACCTTCACCTCCAGCTCCACCTATACC
  CCCGGTGACTGTCCCAACTTTGCGGCTCCCCGCAGAGAGGTGGCACCACCCTATCAGGGG
  GCTGACCCCATCCTTGCGACAGCCCTCGCCTCCGACCCCATCCCCAACCCCCTTCAGAAG
  TGGGAGGACAGCGCCCACAAGCCACAGAGCCTAGACACTGATGACCCCGCGACGCTGTAC
  GCCGTGGTGGAGAACGTGCCCCCGTTGCGCTGGAAGGAATTCGTGCGGCGCCTAGGGCTG
  AGCGACCACGAGATCGATCGGCTGGAGCTGCAGAACGGGCGCTGCCTGCGCGAGGCGCAA
  TACAGCATGCTGGCGACCTGGAGGCGGCGCACGCCGCGGCGCGAGGCCACGCTGGAGCTG
  CTGGGACGCGTGCTCCGCGACATGGACCTGCTGGGCTGCCTGGAGGACATCGAGGAGGCG
  CTTTGCGGCCCCGCCGCCCTCCCGCCCGCGCCCAGTCTTCTCAGATGA
  
  Clone variation with respect to NM_001065.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001065 unedited
  GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCAGAATTCTCTGGACTG
  AGGCTCCAGTTCTGGCCTTTGGGGTTCAAGATCACTGGGACCAGGCCGTGATCTCTATGC
  CCGAGTCTCAACCCTCAACTGTCACCCCAAGGCACTTGGGACGTCCTGGACAGACCGAGT
  CCCGGGAAGCCCCAGCACTGCCGCTGCCACACTGCCCTGAGCCCAAATGGGGGAGTGAGA
  GGCCATAGCTGTCTGGCATGGGCCTCTCCACCGTGCCTGACCTGCTGCTGCCACTGGTGC
  TCCTGGAGCTGTTGGTGGGAATATACCCCTCAGGGGTTATTGGACTGGTCCCTCACCTAG
  GGGACAGGGAGAAGAGAGATAGTGTGTGTCCCCAAGGAAAATATATCCACCCTCAAAATA
  ATTCGATTTGCTGTACCAAGTGCCACAAAGGAACCTACTTGTACAATGACTGTCCAGGCC
  CGGGGCAGGATACGGACTGCAGGGAGTGTGAGAGCGGCTCCTTCACCGCTTCAGAAAACC
  ACCTCAGACACTGCCTCAGCTGCTCCAAATGCCGAAAGGAAATGGGTCAGGTGGAGATCT
  CTTCTTGCACAGTGGACCGGGACACCGTGTGTGGCTGCAGGAAGAACCAGTACCGGCATT
  ATTGGAGTGAAAACCTTTTCCAGTGCTTCAATTGCAGCCTCTGCCTCAATGGGACCGTGC
  ACCTCNTCCTGCCAGAGAAACAGAACACCGTGTGCACCTGCCATGCANNGTTCTTTCTAA
  GAGAAACGAGTGTGTCTCCTGTAGTACTGTAAGAAAAGCTGGAGTGCACGAAGTGTGCCT
  ACCCANATGAGAAATGTAGGGGCACTGAGACTAGCACACAGNNGCTGTGCCCCTGGCATT
  NTCTTGGNNCTTGCCTTTNATCCTCCTCTCATTGGNTAATGTATCGCTACACGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001065 unedited
  CGGCACGCTTTTAGNATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAGCTTT
  AACTTCAAAATTTTAGTGGATGTACAAAAGTCCACAGCTCCAGCTGAAGGCCCCATTGTT
  CCGTGCTCGCCCCTGCCTTAAGACAGTTCAGCTTGCTATGTGCTTGTCCAGGCAGAGGGC
  ACAGGAGTGCCAAGTTTCTATTAGTGTAACATGATTGATTTAAAAACAAAACAAAACAAA
  ACAAAAACAAAAAAAACTGCTTATGCACTGGGAAAAAGGCTCAGGGACGAACCAGGGGCC
  CCCGAGCAGCCTTGCTGGTGAGGACACCCAAAACGGGCATGAGGCATAGCGTCCCTCATC
  CTCGCAAACCACCCACTCAGGCTCTTGAGCCCACGGGGCACCTTTTTTCGCGCGCACAGG
  GCTGACTGTCGGCGGCGCGCAAGCAGCTGAAAAAAGCTATGTACATCGAGGGGTTAGCAC
  CAAGTAGGCGGCTGCTAGCTCCTGCTTGCCCCTGCAGGACCCCTCCTTTCCAGAAAAAAG
  TGGGGTTGGAAGGCGATCTCGCAGGACGGTCCTTATAGCTGCCCGCAAGGGCGCAGCCTC
  ATCTGAGAAAACTGGGCGCGGGCCGGAAGGCGGCCGGGCCCCAAAGCGCCTCCTCGATGT
  TCTCCAGGCAGCCCAACAAGTCCATGTCGCGGAGCACGCGTTCCAACAGCTTCAGCGTGG
  TCTCGCGCCGCCGCGTGCGCCGCCTNCAAGGCGCCAGAATGCTGTATTGCGCCTCGCGCA
  GCAGGGCCCGGTCTGAGCTCCAACCATCGATCTCGGGTTGCTCACCCTAGCGCCGACGAA
  TTCTTCACGCCAAGGGGCCGTTTCCCACGGGTAAGGTCGGGGCCATAATTCTAGCTTGGG
  GTTGGGGCGCTTCCTCCCTCTAAGGGTTGGATAGGTCGAAGCCAGCCTCCCAGTAGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001065
• Insert Size: 2050 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001065.2, NP_001056.1
• RefSeq Size: 2236 bp
• RefSeq ORF: 1368 bp
• Locus ID: 7132
• Cytogenetics: 12p13.31
• Domains: DEATH, TNFR
• Protein Families: Druggable Genome, Secreted Protein, Transcription Factors, Transmembrane
• Protein Pathways: Adipocytokine signaling pathway, Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Cytokine-cytokine receptor interaction, MAPK signaling pathway
• Gene Summary: 'This gene encodes a member of the TNF receptor superfamily of proteins. The encoded receptor is found in membrane-bound and soluble forms that interact with membrane-bound and soluble forms, respectively, of its ligand, tumor necrosis factor alpha. Binding of membrane-bound tumor necrosis factor alpha to the membrane-bound receptor induces receptor trimerization and activation, which plays a role in cell survival, apoptosis, and inflammation. Proteolytic processing of the encoded receptor results in release of the soluble form of the receptor, which can interact with free tumor necrosis factor alpha to inhibit inflammation. Mutations in this gene underlie tumor necrosis factor receptor-associated periodic syndrome (TRAPS), characterized by fever, abdominal pain and other features. Mutations in this gene may also be associated with multiple sclerosis in human patients. [provided by RefSeq, Sep 2016]'
  Transcript Variant: This variant (1) encodes the longest isoform (1).