Spastin (SPAST) (NM_014946) Human Untagged Clone

CAT#: SC128070

SPAST (untagged)-Human spastin (SPAST), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SPAST
• Synonyms: ADPSP; FSP2; SPG4
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_014946, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAATTCTCCGGGTGGACGAGGGAAGAAGAAAGGCTCCGGCGGCGCCAGCAACCCGGTGCCTCCCAGGC
  CTCCGCCCCCTTGCCTGGCCCCCGCCCCTCCCGCCGCCGGGCCGGCCCCTCCGCCCGAGTCGCCGCATAA
  GCGGAACCTGTACTATTTCTCCTACCCGCTGTTTGTAGGCTTCGCGCTGCTGCGTTTGGTCGCCTTCCAC
  CTGGGGCTCCTCTTCGTGTGGCTCTGCCAGCGCTTCTCCCGCGCCCTCATGGCAGCCAAGAGGAGCTCCG
  GGGCCGCGCCAGCACCTGCCTCGGCCTCGGCCCCGGCGCCGGTGCCGGGCGGCGAGGCCGAGCGCGTCCG
  AGTCTTCCACAAACAGGCCTTCGAGTACATCTCCATTGCCCTGCGCATCGATGAGGATGAGAAAGCAGGA
  CAGAAGGAGCAAGCTGTGGAATGGTATAAGAAAGGTATTGAAGAACTGGAAAAAGGAATAGCTGTTATAG
  TTACAGGACAAGGTGAACAGTGTGAAAGAGCTAGACGCCTTCAAGCTAAAATGATGACTAATTTGGTTAT
  GGCCAAGGACCGCTTACAACTTCTAGAGAAGATGCAACCAGTTTTGCCATTTTCCAAGTCACAAACGGAC
  GTCTATAATGACAGTACTAACTTGGCATGCCGCAATGGACATCTCCAGTCAGAAAGTGGAGCTGTTCCAA
  AAAGAAAAGACCCCTTAACACACACTAGTAATTCACTGCCTCGTTCAAAAACAGTTATGAAAACTGGATC
  TGCAGGCCTTTCAGGCCACCATAGAGCACCTAGTTACAGTGGTTTATCCATGGTTTCTGGAGTGAAACAG
  GGATCTGGTCCTGCTCCTACCACTCATAAGGGTACTCCGAAAACAAATAGGACAAATAAACCTTCTACCC
  CTACAACTGCTACTCGTAAGAAAAAAGACTTGAAGAATTTTAGGAATGTGGACAGCAACCTTGCTAACCT
  TATAATGAATGAAATTGTGGACAATGGAACAGCTGTTAAATTTGATGATATAGCTGGTCAAGACTTGGCA
  AAACAAGCATTGCAAGAAATTGTTATTCTTCCTTCTCTGAGGCCTGAGTTGTTCACAGGGCTTAGAGCTC
  CTGCCAGAGGGCTGTTACTCTTTGGTCCACCTGGGAATGGGAAGACAATGCTGGCTAAAGCAGTAGCTGC
  AGAATCGAATGCAACCTTCTTTAATATAAGTGCTGCAAGTTTAACTTCAAAATACGTGGGAGAAGGAGAG
  AAATTGGTGAGGGCTCTTTTTGCTGTGGCTCGAGAACTTCAACCTTCTATAATTTTTATAGATGAAGTTG
  ATAGCCTTTTGTGTGAAAGAAGAGAAGGGGAGCACGATGCTAGTAGACGCCTAAAAACTGAATTTCTAAT
  AGAATTTGATGGTGTACAGTCTGCTGGAGATGACAGAGTACTTGTAATGGGTGCAACTAATAGGCCACAA
  GAGCTTGATGAGGCTGTTCTCAGGCGTTTCATCAAACGGGTATATGTGTCTTTACCAAATGAGGAGACAA
  GACTACTTTTGCTTAAAAATCTGTTATGTAAACAAGGAAGTCCATTGACCCAAAAAGAACTAGCACAACT
  TGCTAGAATGACTGATGGATACTCAGGAAGTGACCTAACAGCTTTGGCAAAAGATGCAGCACTGGGTCCT
  ATCCGAGAACTAAAACCAGAACAGGTGAAGAATATGTCTGCCAGTGAGATGAGAAATATTCGATTATCTG
  ACTTCACTGAATCCTTGAAAAAAATAAAACGCAGCGTCAGCCCTCAAACTTTAGAAGCGTACATACGTTG
  GAACAAGGACTTTGGAGATACCACTGTTTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014946 unedited
  NNNTTTTNNGGGGGGNNNNNNNNNNNNCCCNNNNNATTTAAATACACCCGCCCGTTGNCG
  CAAAGGGCGGTAGGCGTGTACGGTGGNGAGTCTATATAAGCAGAGCTCATTTAGGTGACA
  CTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGGCAGCG
  ACAGGAAGGGAGGGGCCCGAGCCACCGACTGCAGGAGGAGAAGGGGTTGTGCTCCTGGCC
  GAGGAAGGAGAAAGGGGCGGGGCCGGCGGGCAGCGTGCGGCAGTGCGGAGCTCCTGAGAC
  CGGCGGGCACACGGGGGTCTGTGGCCCCCGCCGTAGCAGTGGCTGCCGCCGTCGCTTGGT
  TCCCGTCGGTCTGCGGGAGGCGGGTTATGGCGGCGGCGGCAGTGAGAGCTGTGAATGAAT
  TCTCCGGGTGGACGAGGGAAGAAGAAAGGCTCCGGCGGCGCCAGCAACCCGGTGCCTCCC
  AGGCCTCCGCCCCCTTGCCTGGCCCCCGCCCCTCCCGCCGCCGGGCCGGCCCCTCCGCCC
  GAGTCGCAGCATAAGCGGAACCTGTACTATTTCTCCTACCCGCTGTTTGTAGGCTTCGCG
  CTGCTGCGTTTGGTCGCCTTCCACCTGGGGCTCCTCTTCGTGTGGCTCTGCCAGCGCTTC
  TCCCGCGCCCTCATGGCAGCCAAGAGGAGCTCCGGNGCCGCGCCAGCACCTGCCTCGGCC
  TCGGCCCGGGCGCCGGTGCCGGGCGGCGAGGCCGAGCGCGTCCCGAGTCTTCCACAAACA
  GGCCTTCGAGTACATCTCCATTGCCCTGCGCATCGATGGAAGATGAGCACAGCAGGACAG
  GAAGAGCAAGCTGGTGGAATGTTATAAGAAAGGTTTTTCGAGAACTGGGAAAAGGCAAAA
  GCCTGTTTTAACTTCCGGAACCAGGCGGAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014946 unedited
  GGGAGGCGTTCACTGCATTCAACTTCCACAGCCAGGNAGAGGANTGAGGAGGGGTCACAG
  GGATGCCACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGA
  GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAGTTTATCATTTATTTCCAATACATT
  AGGATAAAAACCCACTTTATGCAACTGTACCCTTTAATTCATTTCTTCTGAAGAAAAGTA
  CAAAAGACCAGACTAAAATTGAGACAAGCATTTAAAGGAAAATCCTTTGACTCAAAGTCC
  AAAACCGAACTGTAAATGGTTCATTTTACCAAAAGAATTAGTTTTTGTACAAAATATAAA
  GAATTCTACACTATAAACAAAGACGGCTGTATATGTAATTAAATTAGAATTCTAGTATCT
  GCTTTTCCTCTAATTCTTCTGGAGTACAAACATATTTGCAAGTCAGAAATCTAATATTCT
  CAATGGACAGACTGTCTTAGAATAAGCTTTTACCCTTAAGATATCTGGAAAGATAGTACT
  CATTCTTTCCCTTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014946
• Insert Size: 5200 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014946.3, NP_055761.2
• RefSeq Size: 5221 bp
• RefSeq ORF: 1851 bp
• Locus ID: 6683
• Cytogenetics: 2p22.3
• Domains: AAA, AAA, MIT
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: 'This gene encodes a member of the AAA (ATPases associated with a variety of cellular activities) protein family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. The use of alternative translational initiation sites in this gene results in a single transcript variant that can produce isoforms that differ in the length of their N-terminus and which thereby differ in the efficiency of their export from the nucleus to the cytoplasm. In addition, alternative splicing results in multiple transcript variants that encode isoforms that differ in other protein regions as well. One isoform of this gene has been shown to be a microtubule-severing enzyme that regulates microtubule abundance, mobility, and plus-end distribution. Mutations in this gene cause the most frequent form of autosomal dominant spastic paraplegia 4. [provided by RefSeq, May 2018]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1; also known as M1). It also encodes a shorter isoform (M87) from an alternate, in-frame, downstream translation initiation site.