CBS (NM_000071) Human Untagged Clone

CAT#: SC128074

CBS (untagged)-Human cystathionine-beta-synthase (CBS), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CBS
• Synonyms: CBSL; HIP4
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000071, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCTTCTGAGACCCCCCAGGCAGAAGTGGGGCCCACAGGCTGCCCCCACCGCTCAGGGCCACACTCGG
  CGAAGGGGAGCCTGGAGAAGGGGTCCCCAGAGGATAAGGAAGCCAAGGAGCCCCTGTGGATCCGGCCCGA
  TGCTCCGAGCAGGTGCACCTGGCAGCTGGGCCGGCCTGCCTCCGAGTCCCCACATCACCACACTGCCCCG
  GCAAAATCTCCAAAAATCTTGCCAGATATTCTGAAGAAAATCGGGGACACCCCTATGGTCAGAATCAACA
  AGATTGGGAAGAAGTTCGGCCTGAAGTGTGAGCTCTTGGCCAAGTGTGAGTTCTTCAACGCGGGCGGGAG
  CGTGAAGGACCGCATCAGCCTGCGGATGATTGAGGATGCTGAGCGCGACGGGACGCTGAAGCCCGGGGAC
  ACGATTATCGAGCCGACATCCGGGAACACCGGGATCGGGCTGGCCCTGGCTGCGGCAGTGAGGGGCTATC
  GCTGCATCATCGTGATGCCAGAGAAGATGAGCTCCGAGAAGGTGGACGTGCTGCGGGCACTGGGGGCTGA
  GATTGTGAGGACGCCCACCAATGCCAGGTTCGACTCCCCGGAGTCACACGTGGGGGTGGCCTGGCGGCTG
  AAGAACGAAATCCCCAATTCTCACATCCTAGACCAGTACCGCAACGCCAGCAACCCCCTGGCTCACTACG
  ACACCACCGCTGATGAGATCCTGCAGCAGTGTGATGGGAAGCTGGACATGCTGGTGGCTTCAGTGGGCAC
  GGGCGGCACCATCACGGGCATTGCCAGGAAGCTGAAGGAGAAGTGTCCTGGATGCAGGATCATTGGGGTG
  GATCCCGAAGGGTCCATCCTCGCAGAGCCGGAGGAGCTGAACCAGACGGAGCAGACAACCTACGAGGTGG
  AAGGGATCGGCTACGACTTCATCCCCACGGTGCTGGACAGGACGGTGGTGGACAAGTGGTTCAAGAGCAA
  CGATGAGGAGGCGTTCACCTTTGCCCGCATGCTGATCGCGCAAGAGGGGCTGCTGTGCGGTGGCAGTGCT
  GGCAGCACGGTGGCGGTGGCCGTGAAGGCCGCGCAGGAGCTGCAGGAGGGCCAGCGCTGCGTGGTCATTC
  TGCCCGACTCAGTGCGGAACTACATGACCAAGTTCCTGAGCGACAGGTGGATGCTGCAGAAGGGCTTTCT
  GAAGGAGGAGGACCTCACGGAGAAGAAGCCCTGGTGGTGGCACCTCCGTGTTCAGGAGCTGGGCCTGTCA
  GCCCCGCTGACCGTGCTCCCGACCATCACCTGTGGGCACACCATCGAGATCCTCCGGGAGAAGGGCTTCG
  ACCAGGCGCCCGTGGTGGATGAGGCGGGGGTAATCCTGGGAATGGTGACGCTTGGGAACATGCTCTCGTC
  CCTGCTTGCCGGGAAGGTGCAGCCGTCAGACCAAGTTGGCAAAGTCATCTACAAGCAGTTCAAACAGATC
  CGCCTCACGGACACGCTGGGCAGGCTCTCGCACATCCTGGAGATGGACCACTTCGCCCTGGTGGTGCACG
  AGCAGATCCAGTACCACAGCACCGGGAAGTCCAGTCAGCGGCAGATGGTGTTCGGGGTGGTCACCGCCAT
  TGACTTGCTGAACTTCGTGGCCGCCCAGGAGCGGGACCAGAAGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000071 unedited
  TACTATAGGGCGGCCGCGATTCGGCACGAGGGCCGCAGTCGGGGCAGCCGCTCGCCCCTC
  TTTTCCATGTATCCGTCCAGGATCCCATGACAGATTCTGTTGTCACGTCTCCTTACAGAG
  TTTGAGCGGTGCTGAACTGTCAGCACCATCTGTCCGGTCCCAGCATGCCTTCTGAGACCC
  CCCAGGCAGAAGTGGGGCCCACAGGCTGCCCCCACCGCTCAGGGCCACACTCGGCGAAGG
  GGAGCCTGGAGNAAGGGGTCCCCAGAGGATAAGGAAGCCAAGGGAGCCCCTGTGGATCCG
  GCCCGATGCTCCGAGCAGGTGCACCTGGCAGCTGGGCCGGCCTGCCTCCGAGTCCCCACA
  TCACCACACTGCCCCGGCAAAATCTCCAAAAATCTTGCCAGATATTCTGAAGAAAATCGG
  GGACACCCCTATGGTCAGAATAACAAGATGGGAAAGAAGTTCGGCCTGAAGTGTGAGCTC
  TTGGCCAAGTGGTGAGTTCTTCAACGCGGGCGGGAGCGTGAAGGACCGCATCAGCCTGCG
  GATGATNTGAGGATGCTGAGCGCGACGGGACGCTGAAGCCCGGNGACACGATTATCGAGC
  CGACATCCGGGACACCCGGGATCCGGGCTTGCCCCTGCTGCNGCANNGGAAGGGGCTATC
  GCTGCATCATCGTGATGCCAGAGAGATGACTCCAGAGGGGGGGGACTGGCTGCGGCCCTG
  GGGGCTTTGAAATGGTGAGGACGCCCACCATGGCCAGTTCGACTCCCCGATCCTNNNNGN
  GNGGGGGGGGGCCCCGGCGGCTTAAAAAAAAAAACCCCATTTTTCACATCCTAAACCAGA
  CCGGAACGCAGAACCCCCTGGTTTATATACACACCGTTGATAAATCCCGCACATGGGATG
  GGAAACTGAATTCCTGGGGCTTTATGGCCCCGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000071 unedited
  NNNTTTTTGCTTATGGACCGCGGNCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTT
  GTGTGATTCCATTTATATGAAATGTCCAGNACAGGGGAAAACCTATTTTAGACAACAGAG
  ACACAAAGTCGATCAGCAGTTGCCAGGGGAGGAGGAAGACGGGAGGGGAAATGATTGCTT
  CACAGGGTGATGACAGAATGTTCCGGAACGTGACAGAGGTGGTGCCTACACAACTTTCTG
  GATGTACTAAATGCCGCTGATTGTTCACTTTCAAGTGATTGATTTTTAGGTTATTTGAAT
  TTCATCTCAATTAAAAAACCCAAACACGCAAACTGCTCCCGCCAGCTCAGCCCCGAGCAG
  ACGGCGCAGCCCGTGGAGGATGCTGAGCCACCCAGGCTTCTCCCCGCCCCTTCCTGGACT
  CTTCTCTCTTTTGCCTTTAATCCACTCTGCGCAGTCATTGCCTGTGTTCATCCTACCTGG
  CCGACTTCTCTCTCAATATTTCCTTGGTGAAACATCCTAAGAGGAAGTTACATTAGAGAA
  AATCACATATTTCATCTCATAGGCCGTAAACAGGGCCATTTAGGAAGCCATGTGTTAACG
  AGGGGAGACGGATGCTCTGTGCCGTGTGCAGGGATAACGGTGCCAGGCCAGGCAGTTACC
  AATCACGCGTGTGTTTAGGGCTCANGAAAGCGAAGGAGAAGTGGGCAAGGGTGGNGGGCC
  CGCTTCGCACCGNCCAGNGCTTCGGACTTCACTTCTGGTCCCGCTCCTGGGCGGCCACGA
  AGTTAGCAAGTCAATGGCGGTGACCACCCCGAACCCCTCTGNCGCTGACTGGACTTCCCG
  NTGCTGGGGTACTGGATCTGCTCGTGCACCACCAGGGCCAAATGGGTCCTCTCCAGAATT
  GCCAAAACCCTGCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000071
• Insert Size: 2360 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000071.1, NP_000062.1
• RefSeq Size: 2544 bp
• RefSeq ORF: 1656 bp
• Locus ID: 875
• Cytogenetics: 21q22.3
• Domains: CBS, PALP
• Protein Families: Druggable Genome
• Protein Pathways: Cysteine and methionine metabolism, Glycine, serine and threonine metabolism, Metabolic pathways, Selenoamino acid metabolism
• Gene Summary: 'The protein encoded by this gene acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. The encoded protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria. This gene is a major contributor to cellular hydrogen sulfide production. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2016]'