SURF4 (NM_033161) Human Untagged Clone

CAT#: SC128236

SURF4 (untagged)-Human surfeit 4 (SURF4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SURF4
• Synonyms: ERV29
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC128236 sequence for NM_033161 edited (data generated by NextGen Sequencing)
  ATGGGCCAGAACGACCTGATGGGCACGGCCGAGGACTTCGCCGACCAGTTCCTCCGTGTC
  ACAAAGCAGTACCTGCCCCACGTGGCGCGCCTCTGTCTGATCAGCACCTTCCTGGAGGAC
  GGCATCCGTATGTGGTTCCAGTGGAGCGAGCAGCGCGACTACATCGACACCACCTGGAAC
  TGCGGCTACCTGCTGGCCTCGTCCTTCGTCTTCCTCAACTTGCTGGGACAGCTGACTGGC
  TGCGTCCTGGTGTTGAGCAGGAACTTCGTGCAGTACGCCTGCTTCGGGCTCTTTGGAATC
  ATAGCTCTGCAGACGATTGCCTACAGCATTTTATGGGACTTGAAGTTTTTGATGAGGAAC
  CTGGCCCTGGGAGGAGGCCTGTTGCTGCTCCTAGCAGAATCCCGTTCTGAAGGGAAGAGC
  ATGTTTGCGGGCGTCCCCACCATGCGTGAGAGCTCCCCCAAACAGTACATGCAGCTCGGA
  GGCAGGGTCTTGCTGGTTCTGATGTTCATGACCCTCCTTCACTTTGACGCCAGCTTCTTT
  TCTATTGTCCAGAACATCGTGGGCACAGCTCTGATGATTTTAGTGGCCATTGGTTTTAAA
  ACCAAGCTGGCTGCTTTGACTCTTGTTGTGTGGCTCTTTGCCATCAACGTATATTTCAAC
  GCCTTCTGGACCATTCCAGTCTACAAGCCCATGCATGACTTCCTGAAATACGACTTCTTC
  CAGACCATGTCGGTGATTGGGGGCTTGCTCCTGGTGGTGGCCCTGGGCCCTGGGGGTGTC
  TCCATGGATGAGAAGAAGAAGGAGTGGTAA
  
  Clone variation with respect to NM_033161.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_033161 unedited
  NCGGTTCAAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACCAGCGGGCGCC
  GACGGGCGAGAGCCAGCGAGCGAGCGAGCGAGCCGAGCCGAGCCTCCCGCCGTCGCCATG
  GGCCAGAACGACCTGATGGGCACGGCCGAGGACTTCGCCGACCAGTTCCTCCGTGTCACA
  AAGCAGTACCTGCCCCACGTGGCGCGCCTCTGTCTGATCAGCACCTTCCTGGAGGACGGC
  ATCCGTATGTGGTTCCAGTGGAGCGAGCAGCGCGACTACATCGACACCACCTGGAACTGC
  GGCTACCTGCTGGCCTCGTCCTTCGTCTTCCTCAACTTGCTGGGACAGCTGACTGGCTGC
  GTCCTGGTGTTGAGCAGGAACTTCGTGCAGTACGCCTGCTTCGGGCTCTTTGGAATCATA
  GCTCTGCAGACGATTGCCTACAGCATTTTATGGGACTTGAAGTTTTTGATGAGGAACCTG
  GCCCTGGGAGGAGGCCTGTTGCTGCTCCTAGCAGAATCCCGTTCTGAAGGGAAGAGCATG
  TTTGCGGGCGTCCCCACCATGCGTGAGAGCTCCCCCAAACAGTACATGCAGCTCGGAGGC
  AGGGTCTTGCTGGTTCTGATGTTCATGACCCTCCTTCACTTTGACGCCAGCTTCTTTTCT
  ATTGTCCAGAACATCGTGGGCACAGCTCTGATGATTTTAGTGGCCATTGGTTTTAAAACC
  AAGCTGGCTGCTTTGACTCTTGTTGTGTGGCTCTTTGCCATCAACGTATATTTCAACGCC
  TTCTGGACCATTCCAGTCTACAGCCCATGCATGACTTCCTGAATACGACTTCTTCAACCA
  TGTCNGTGAATTGGNGGCTGCTCTGGGNGGTGGCCTGCCCTGCGGGGGGGTGCTCCTGGA
  TGAGAAAAAAAAGGATGGGTACAC
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_033161 unedited
  GGAAAAGTGTCTTGCTGGTTNTGNAGTTCATAACCCTCCTTCATTTNGAGCCAGCTTCTT
  TTCTATTGTCCAGAACATCGTGGGCACAGCTCTGATGATTTTAGTGGCCATTGGTTTTAA
  AACCAAGCTGGCTGCTTTGACTCTTGTTGTGTGGCTCTTTGCCATCAACGTATATTTCAA
  CGCCTTCTGGACCATTCCAGTCTACAAGCCCATGCATGACTTCCTGAAATACGACTTCTT
  CCAGACCATGTCGGTGATTGGGGGCTTGCTCCTGGTGGTGGCCCTGGGCCCTGGGGGTGT
  CTCCATGGATGAGAAGAAGAAGGAGTGGTAACAGTCACAGATCCCTACCTGCCTGGCTAA
  GACCCGTGGCCGTCAAGGACTGGTTCGGGGTGGATTCAACAAAACTGCCAGCTTTTATGT
  ATCCTCTTCCCTTCCCCTCCCTTGGTAAAGGCACAGATGTTTTGAGAACTTTATTTGCAG
  AGACACCTGAGAATCGATGGCTCAGTCTGCTCTGGAGCCACAGTCTGGCGTCTGACCCTT
  CAGTGCAGGCCAGCCTGGCAGCTGGAAGCCTCCCCCACGCCGAGGCTTTGGAGTGAACAG
  CCCGCTTGGCTGTGGCATCTCAGTCCTATTTTTGAGTTTTTTTGTGGGGGTACAGGAGGG
  GGCCTTCAAGCTGTACTGTGAGCAGACGCATTGGTATTATCATTCANAGCAGTCTCCCTC
  TTATTTGTAAGTTTACATTTTTAGCGGAAACTACTAAATTATTTTGGGGTGGTTCAGCCA
  AACCTCANAACAGTTAANTCTCCCTGGTTTAAAATCACACCAGTGGCTTTGATGTTGTTT
  CTGCCCCGCATTGTATTTTATAGGAATACTNGAAACATTTAGGNACACCCAAAAATGATG
  CA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_033161
• Insert Size: 2800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033161.2, NP_149351.1
• RefSeq Size: 2985 bp
• RefSeq ORF: 810 bp
• Locus ID: 6836
• Cytogenetics: 9q34.2
• Domains: SURF4
• Protein Families: Transmembrane
• Gene Summary: 'This gene is located in the surfeit gene cluster, which is comprised of very tightly linked housekeeping genes that do not share sequence similarity. The encoded protein is a conserved integral membrane protein that interacts with endoplasmic reticulum-Golgi intermediate compartment proteins. Disruption of this gene results in reduced numbers of endoplasmic reticulum-Golgi intermediate compartment clusters and redistribution of coat protein I to the cytosol. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]'
  Transcript Variant: This variant (1) encodes the longest isoform (1).