GEM (NM_005261) Human Untagged Clone

CAT#: SC128239

GEM (untagged)-Human GTP binding protein overexpressed in skeletal muscle (GEM), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GEM
• Synonyms: KIR
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_005261 edited
  ATGACTCTGAATAATGTCACCATGCGCCAGGGCACTGTGGGCATGCAGCCACAGCAGCAG
  CGCTGGAGCATCCCAGCTGATGGCAGGCATCTGATGGTCCAGAAAGAGCCCCACCAGTAC
  AGCCACCGCAACCGCCATTCTGCTACCCCTGAGGACCACTGCCGCCGAAGCTGGTCCTCT
  GACTCCACAGACTCAGTCATCTCCTCTGAGTCAGGGAACACCTACTACCGAGTGGTGCTC
  ATAGGGGAGCAGGGGGTGGGCAAGTCCACTCTGGCCAACATCTTTGCAGGTGTGCATGAC
  AGCATGGACAGCGACTGCGAGGTGCTGGGAGAAGATACATATGAACGAACCCTGATGGTT
  GATGGGGAAAGTGCAACGATTATACTCCTGGATATGTGGGAAAATAAGGGGGAAAATGAA
  TGGCTCCATGACCACTGCATGCAGGTCGGGGACGCATACCTGATTGTCTACTCAATCACA
  GACCGAGCGAGCTTCGAGAAGGCATCTGAGCTGCGAATCCAGCTCCGCAGGGCCCGGCAG
  ACAGAGGACATTCCCATAATTTTGGTTGGCAACAAAAGTGACTTAGTGCGGTGCCGAGAA
  GTGTCTGTATCAGAAGGGAGAGCCTGTGCAGTGGTGTTTGACTGCAAGTTCATCGAGACC
  TCTGCAGCTGTCCAGCACAACGTGAAGGAGCTGTTTGAGGGCATTGTGCGACAGGTGCGC
  CTTCGGCGGGACAGCAAGGAGAAGAATGAACGGCGGCTGGCCTACCAGAAAAGGAAGGAG
  AGCATGCCCAGGAAAGCCAGGCGCTTCTGGGGCAAGATCGTGGCCAAAAACAACAAGAAT
  ATGGCCTTCAAGCTCAAGTCCAAATCCTGCCATGACCTCTCTGTACTCTAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005261 unedited
  AAAAACAACCCCTGGNAACGTGCGCTCAGCACTGGGATTTTCTGGACTCAACGATGACTC
  TGAATAATGTCACCATGCGCCAGGGCACTGTGGGCATGCAGCCACAGCAGCAGCGCTGGA
  GCATCCCAGCTGATGGCAGGCATCTGATGGTCCAGAAAGAGCCCCACCAGTACAGCCACC
  GCAACCGCCATTCTGCTACCCCTGAGGACCACTGCCGCCGAAGCTGGTCCTCTGACTCCA
  CAGACTCAGTCATCTCCTCTGAGTCAGGGAACACCTACTACCGAGTGGTGCTCATAGGGG
  AGCAGGGGGTGGGCAAGTCCACTCTGGCCAACATCTTTGCAGGTGTGCATGACAGCATGG
  ACAGCGACTGCGAGGTGCTGGGAGAAGATACATATGAACGAACCCTGATGGTTGATGGGG
  AAAGTGCAACGATTATACTCCTGGATATGTGGGAAAATAAGGGGGAAAATGAATGGCTCC
  ATGACCACTGCATGCAGGTCGGGGACGCATACCTGATTGTCTACTCAATCACAGACCGAG
  CGAGCTTCGAGAAGGCATCTGAGCTGCGAATCCAGCTCCGCAGGGCCCGGCAGACAGAGG
  ACATTCCCATAATTNTGGTTGGCAACANAAGTGACTTAGTGCGGTGCCGAGAAGTGTCTG
  TATCAGAAGGGAGAGCCTGTGCAGTGGGTGTTGACTGCAAGTTCATCGAGACCTCTGCAG
  CTGTCCAGCACAACGTGAAAGAGCTGTTTGAGGGCATTGTGCGACAGGTGCGCCCTCGGC
  GGNACAGNCAGGAGAGGATGAACGGCGGCTGGCCTACCAGAAAAGAAGGANACATGCCCA
  GNAAAGCAGCCGCTTCTGGGGCAGATCGTGCCAAAACAACAGAATATGCCTTAA
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_005261 unedited
  GCTTAATGCACANACCCACGATTTCGANAAGCATCTAGGCTGCGAATCCAGCTCCGCAGG
  GCCCGGCAGACAGAGGACATTCCCATAATTTTGGTTGGCAACAAAAGTGACTTAGTGCGG
  TGCCGAGAAGTGTCTGTATCAGAAGGGAGAGCCTGTGCAGTGGTGTTTGACTGCAAGTTC
  ATCGAGACCTCTGCAGCTGTCCAGCACAACGTGAAGGAGCTGTTTGAGGGCATTGTGCGA
  CAGGTGCGCCTTCGGCGGGACAGCAAGGAGAAGAATGAACGGCGGCTGGCCTACCAGAAA
  AGGAAGGAGAGCATGCCCAGGAAAGCCAGGCGCTTCTGGGGCAAGATCGTGGCCAAAAAC
  AACAAGAATATGGCCTTCAAGCTCAAGTCCAAATCCTGCCATGACCTCTCTGTACTCTAG
  GAACCCAGGGTCACCCAGATGTCCCTTTGATGGCCGTTGTTGAAGGCCATTGGGACCAAT
  AATCTATATTAGATTGAATACTTAAGTTAGATGTGGTTTCCCCCATTGTAGCAGGGAGCT
  AGCGTATTAGCCTTGTGGGCAACATGATGCATGGGAAATGAAAGATTTTTGTAAAAAGTC
  AGTATTTATTTCCAGGAAAAGCCTGACCTTGCTATTTGAACACCCAAGACTCTTTAGAGG
  ATGTNGTTGGTGTTCACATGTGTTTCTTCTATTTTGGATAGTAGGGAAGTAAAGCTTACA
  AAGAATGCCTAGNNACAGAACTTTCATCATTAAAATTTTTCCCAGTGGTCTGATATGTGA
  CTTTGAAGCAATGAGTCATAACAATATAGAAAGCTGTCATGAGTTCTTCAAAGGAGGAAA
  ACTTCTACGATCTTAGATCATGGACTAGNATGGGGACTAGCTCACAGATCGTGACTATAT
  GCTCCTCCACTGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005261
• Insert Size: 2100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005261.2, NP_005252.1
• RefSeq Size: 2205 bp
• RefSeq ORF: 891 bp
• Locus ID: 2669
• Cytogenetics: 8q22.1
• Domains: ras, RAS, RHO, RAB
• Protein Families: Druggable Genome
• Gene Summary: 'The protein encoded by this gene belongs to the RAD/GEM family of GTP-binding proteins. It is associated with the inner face of the plasma membrane and could play a role as a regulatory protein in receptor-mediated signal transduction. Alternative splicing occurs at this locus and two transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.