gamma Sarcoglycan (SGCG) (NM_000231) Human Untagged Clone

CAT#: SC300030

SGCG (untagged)-Human sarcoglycan, gamma (35kDa dystrophin-associated glycoprotein) (SGCG)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SGCG
• Synonyms: 35DAG; A4; DAGA4; DMDA; DMDA1; gamma-SG; LGMD2C; LGMDR5; MAM; SCARMD2; SCG3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000231, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGTGCGTGAGCAGTACACTACAGCCACAGAAGGCATCTGCATAGAGAGGCCAGAGAATCAGTATGTCT
  ACAAAATTGGCATTTATGGCTGGAGAAAGCGCTGTCTCTACTTGTTTGTTCTTCTTTTACTCATCATCCT
  CGTTGTGAATTTAGCTCTTACAATTTGGATTCTTAAAGTGATGTGGTTTTCTCCAGCAGGAATGGGCCAC
  TTGTGTGTAACAAAAGATGGACTGCGCTTGGAAGGGGAATCAGAATTTTTATTCCCATTGTATGCCAAAG
  AAATACACTCCAGAGTGGACTCATCTCTGCTTCTACAATCAACCCAGAATGTGACTGTAAATGCGCGCAA
  CTCAGAAGGGGAGGTCACAGGCAGGTTAAAAGTCGGTCCCAAAATGGTAGAAGTCCAGAATCAACAGTTT
  CAGATCAACTCCAACGACGGCAAGCCACTATTTACTGTAGATGAGAAGGAAGTTGTGGTTGGTACAGATA
  AACTTCGAGTAACTGGGCCTGAAGGGGCTCTTTTTGAACATTCAGTGGAGACACCCCTTGTCAGAGCCGA
  CCCGTTTCAAGACCTTAGATTAGAATCCCCCACTCGGAGTCTAAGCATGGATGCCCCAAGGGGTGTGCAT
  ATTCAAGCTCACGCTGGGAAAATTGAGGCGCTTTCTCAAATGGATATTCTTTTTCATAGTAGTGATGGAA
  TGCTTGTGCTTGATGCTGAAACTGTGTGCTTACCCAAGCTGGTGCAGGGGACGTGGGGTCCCTCTGGCAG
  CTCACAGAGCCTCTACGAAATCTGTGTGTGTCCAGATGGGAAGCTGTACCTGTCTGTGGCCGGTGTGAGC
  ACCACGTGCCAGGAGCACAGCCACATCTGCCTCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_000231
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000231.2, NP_000222.1
• RefSeq Size: 1661 bp
• RefSeq ORF: 876 bp
• Locus ID: 6445
• Cytogenetics: 13q12.12
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM), Viral myocarditis
• Gene Summary: 'This gene encodes gamma-sarcoglycan, one of several sarcolemmal transmembrane glycoproteins that interact with dystrophin. The dystrophin-glycoprotein complex (DGC) spans the sarcolemma and is comprised of dystrophin, syntrophin, alpha- and beta-dystroglycans and sarcoglycans. The DGC provides a structural link between the subsarcolemmal cytoskeleton and the extracellular matrix of muscle cells. Defects in the encoded protein can lead to early onset autosomal recessive muscular dystrophy, in particular limb-girdle muscular dystrophy, type 2C (LGMD2C). [provided by RefSeq, Oct 2008]'