HNF1 alpha (HNF1A) (NM_000545) Human Untagged Clone

CAT#: SC300093

HNF1A (untagged)-Human HNF1 homeobox A (HNF1A)


  "NM_000545" in other vectors (6)

Reconstitution Protocol

USD 760.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol HNF1A
Synonyms HNF-1A; HNF1; HNF4A; IDDM20; LFB1; MODY3; TCF-1; TCF1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_000545 edited
CGTGGCCCTGTGGCAGCCGAGCCATGGTTTCTAAACTGAGCCAGCTGCAGACGGAGCTCC
TGGCGGCCCTGCTCGAGTCAGGGCTGAGCAAAGAGGCACTGATCCAGGCACTGGGTGAGC
CGGGGCCCTACCTCCTGGCTGGAGAAGGCCCCCTGGACAAGGGGGAGTCCTGCGGCGGCG
GTCGAGGGGAGCTGGCTGAGCTGCCCAATGGGCTGGGGGAGACTCGGGGCTCCGAGGACG
AGACGGACGACGATGGGGAAGACTTCACGCCACCCATCCTCAAAGAGCTGGAGAACCTCA
GCCCTGAGGAGGCGGCCCACCAGAAAGCCGTGGTGGAGACCCTTCTGCAGGAGGACCCGT
GGCGTGTGGCGAAGATGGTCAAGTCCTACCTGCAGCAGCACAACATCCCACAGCGGGAGG
TGGTCGATACCACTGGCCTCAACCAGTCCCACCTGTCCCAACACCTCAACAAGGGCACTC
CCATGAAGACGCAGAAGCGGGCCGCCCTGTACACCTGGTACGTCCGCAAGCAGCGAGAGG
TGGCGCAGCAGTTCACCCATGCAGGGCAGGGAGGGCTGATTGAAGAGCCCACAGGTGATG
AGCTACCAACCAAGAAGGGGCGGAGGAACCGTTTCAAGTGGGGCCCAGCATCCCAGCAGA
TCCTGTTCCAGGCCTATGAGAGGCAGAAGAACCCTAGCAAGGAGGAGCGAGAGACGCTAG
TGGAGGAGTGCAATAGGGCGGAATGCATCCAGAGAGGGGTGTCCCCATCACAGGCACAGG
GGCTGGGCTCCAACCTCGTCACGGAGGTGCGTGTCTACAACTGGTTTGCCAACCGGCGCA
AAGAAGAAGCCTTCCGGCACAAGCTGGCCATGGACACGTACAGCGGGCCCCCCCCAGGGC
CAGGCCCGGGACCTGCGCTGCCCGCTCACAGCTCCCCTGGCCTGCCTCCACCTGCCCTCT
CCCCCAGTAAGGTCCACGGTGTGCGCTATGGACAGCCTGCGACCAGTGAGACTGCAGAAG
TACCCTCAAGCAGCGGCGGTCCCTTAGTGACAGTGTCTACACCCCTCCACCAAGTGTCCC
CCACGGGCCTGGAGCCCAGCCACAGCCTGCTGAGTACAGAAGCCAAGCTGGTCTCAGCAG
CTGGGGGCCCCCTCCCCCCTGTCAGCACCCTGACAGCACTGCACAGCTTGGAGCAGACAT
CCCCAGGCCTCAACCAGCAGCCCCAGAACCTCATCATGGCCTCACTTCCTGGGGTCATGA
CCATCGGGCCTGGTGAGCCTGCCTCCCTGGGTCCTACGTTCACCAACACAGGTGCCTCCA
CCCTGGTCATCGGCCTGGCCTCCACGCAGGCACAGAGTGTGCCGGTCATCAACAGCATGG
GCAGCAGCCTGACCACCCTGCAGCCCGTCCAGTTCTCCCAGCCGCTGCACCCCTCCTACC
AGCAGCCGCTCATGCCACCTGTGCAGAGCCATGTGACCCAGAGCCCCTTCATGGCCACCA
TGGCTCAGCTGCAGAGCCCCCACGCCCTCTACAGCCACAAGCCCGAGGTGGCCCAGTACA
CCCACACGGGCCTGCTCCCGCAGACTATGCTCATCACCGACACCACCAACCTGAGCGCCC
TGGCCAGCCTCACGCCCACCAAGCAGGTCTTCACCTCAGACACTGAGGCCTCCAGTGAGT
CCGGGCTTCACACGCCGGCATCTCAGGCCACCACCCTCCACGTCCCCAGCCAGGACCCTG
CCGGCATCCAGCACCTGCAGCCGGCCCACCGGCTCAGCGCCAGCCCCACAGTGTCCTCCA
GCAGCCTGGTGCTGTACCAGAGCTCAGACTCCAGCAATGGCCAGAGCCACCTGCTGCCAT
CCAACCACAGCGTCATCGAGACCTTCATCTCCACCCAGATGGCCTCTTCCTCCCAGTAAC
CACGGCACCTGGGCCCTGGGGCCTGTACTGCCTGCTTGGGGGGTGATGAGGGCAGCAGCC
AGCCCTGCCTGGAGGACCTGAGCCTGCCGAGCAACCGTGGCCCTTCCTGGACAGCTGTGC
CTCGCTCCCCACTCTGCTCTGATGCATCAGAAAGGGAGGGCTCTGA
>OriGene 5' read for NM_000545 unedited
GGGCTCAGATTTGTAAACGACTTATATAGGGCGGCCGCGAATCCGAGCCATGGTTTCTAA
ACTGAGCCAGCTGCAGACGGAGCTCCTGGCGGCCCTGCTCGAGTCAGGGCTGAGCAAAGA
GGCACTGATCCAGGCACTGGGTGAGCCGGGGCCCTACCTCCTGGCTGGAGAAGGCCCCCT
GGACAAGGGGGAGTCCTGCGGCGGCGGTCGAGGGGAGCTGGCTGAGCTGCCCAATGGGCT
GGGGGAGACTCGGGGCTCCGAGGACGAGACGGACGACGATGGGGAAGACTTCACGCCACC
CATCCTCAAAGAGCTGGAGAACCTCAGCCCTGAGGAGGCGGCCCACCAGAAAGCCGTGGT
GGAGACCCTTCTGCAGGAGGACCCGTGGCGTGTGGCGAAGATGGTCAAGTCCTACCTGCA
GCAGCACAACATCCCACAGCGGGAGGTGGTCGATACCACTGGCCTCAACCAGTCCCACCT
GTCCCAACACCTCAACAAGGGCACTCCCATGAAGACGCAGAAGCGGGCCGCCCTGTACAC
CTGGTACGTCCGCAAGCAGCGAGAGGTGGCGCAGCAGTTCACCCATGCAGGGCAGGGAGG
GCTGATTGAAGAGCCCACAGGTGATGAGCTACCAACCAAGAAGGNGCGGAGGAACCGTTT
CAAGTGGGGCCCAGCATCCCAGCAGATCCTGTTCCAGGCCTATGAGAGGCAGAAGAACCC
TAGCAAGGAGAGCGAGAGACGCTAGTGGAGGAGTGCAATAGGGCGGAATGCATCCAGAGA
GGGGTGGTCCCATCACAGGCACAGGGGCTGGGGCTCAACCTCGTCACGGGAGTGCGTGTC
TACAACTGGTTTGGCACCGGCGCAAAGAAGAAGCCTTTCCGCCCAAGCTGGCCCTGGAC
>OriGene 3' read for NM_000545 unedited
CCACGTAGACTGCGCCTCGCAAGCTAGGTACAGCACCGTGGATAGGGTTCACAAGGTACT
CACATCGGCATCGGTGTACGAATCAANAGCCCTCCCTTTCTGATGCATCAGAGCAGAGTG
GGGAGCGAGGCACAGCTGTCCAGGAAGGGCCACGGTTGCTCGGCAGGCTCATGTCCTCCA
GGCACGGCTGGCTGCTGCCCTCATCACCCCCCAAGCAGGCAGTACAGGCCCCAGGGCCCA
CGTGCCGTGGTTACTGGGAGGAAGAGGCCATCTGGGTGGAGATGAAGGTCTCGATGACGC
TGTGGTTGGATGGCAGCAAGTGGCTCTGGCCATTGCTGGAGTCTGACCTCTGGTACAGCA
CCAGGCTGCTGGAGGACACTGTGGGGCTGGCGCTGAGCCGGTGGGCCGGCTGCAGGTGCT
GGATGCCTGCAGGGTCCTGGCTGGCGACTTGAAGGGTGGTGGCCTGAGATGCCGGCCTGT
GAAGCCCGGACTCACTGGAGGCCTCACTGTCTGACGTGAACACCTGCTTGGTGGGCGTGA
GGCTGGCCATGGCGCTCAGGTTGGTGGTGTCTGTGATGAGCATACTCTGCGTGAGCAGGC
ACCTGTGTGTGTACTGTGCCACCTCGGGCTTGTGGCTGTATAGGGCGTGGCGGCTCTGCA
ACTGATCCATGGTGGCCATCGAAGGGGCTCTGGGGTCACATCGACTCTGCACATGTGGCA
TTGACCGGCTCGCTGGTACGTATGGGTGCCAACCGCTGGGGAGAACTTGAACCGGGCTGC
AGGGTGGATCAGGGCTGCTGCCCATGCTGGTTGATGACCCGGCACAACTCTGTGGCTTGC
CTTGAAGGGCCAGAACCCATGACCCATGGGTGGAAGGCCACCGGGGGTGGTTGGAAACGT
CAGGACCCAAGGGGAGGCCAAGCTTCACCAGCCCCCCATGGTGCCATAA
Restriction Sites Please inquire     
ACCN NM_000545
Insert Size 2100 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000545.3, NP_000536.3
RefSeq Size 3249 bp
RefSeq ORF 1896 bp
Locus ID 6927
Cytogenetics 12q24.31
Protein Families Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
Protein Pathways Maturity onset diabetes of the young
Gene Summary 'The protein encoded by this gene is a transcription factor required for the expression of several liver-specific genes. The encoded protein functions as a homodimer and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Defects in this gene are a cause of maturity onset diabetes of the young type 3 (MODY3) and also can result in the appearance of hepatic adenomas. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]'
Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.

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