HNF1 alpha (HNF1A) (NM_000545) Human Untagged Clone

CAT#: SC300093

HNF1A (untagged)-Human HNF1 homeobox A (HNF1A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HNF1A
• Synonyms: HNF-1A; HNF1; HNF4A; IDDM20; LFB1; MODY3; TCF-1; TCF1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000545 edited
  CGTGGCCCTGTGGCAGCCGAGCCATGGTTTCTAAACTGAGCCAGCTGCAGACGGAGCTCC
  TGGCGGCCCTGCTCGAGTCAGGGCTGAGCAAAGAGGCACTGATCCAGGCACTGGGTGAGC
  CGGGGCCCTACCTCCTGGCTGGAGAAGGCCCCCTGGACAAGGGGGAGTCCTGCGGCGGCG
  GTCGAGGGGAGCTGGCTGAGCTGCCCAATGGGCTGGGGGAGACTCGGGGCTCCGAGGACG
  AGACGGACGACGATGGGGAAGACTTCACGCCACCCATCCTCAAAGAGCTGGAGAACCTCA
  GCCCTGAGGAGGCGGCCCACCAGAAAGCCGTGGTGGAGACCCTTCTGCAGGAGGACCCGT
  GGCGTGTGGCGAAGATGGTCAAGTCCTACCTGCAGCAGCACAACATCCCACAGCGGGAGG
  TGGTCGATACCACTGGCCTCAACCAGTCCCACCTGTCCCAACACCTCAACAAGGGCACTC
  CCATGAAGACGCAGAAGCGGGCCGCCCTGTACACCTGGTACGTCCGCAAGCAGCGAGAGG
  TGGCGCAGCAGTTCACCCATGCAGGGCAGGGAGGGCTGATTGAAGAGCCCACAGGTGATG
  AGCTACCAACCAAGAAGGGGCGGAGGAACCGTTTCAAGTGGGGCCCAGCATCCCAGCAGA
  TCCTGTTCCAGGCCTATGAGAGGCAGAAGAACCCTAGCAAGGAGGAGCGAGAGACGCTAG
  TGGAGGAGTGCAATAGGGCGGAATGCATCCAGAGAGGGGTGTCCCCATCACAGGCACAGG
  GGCTGGGCTCCAACCTCGTCACGGAGGTGCGTGTCTACAACTGGTTTGCCAACCGGCGCA
  AAGAAGAAGCCTTCCGGCACAAGCTGGCCATGGACACGTACAGCGGGCCCCCCCCAGGGC
  CAGGCCCGGGACCTGCGCTGCCCGCTCACAGCTCCCCTGGCCTGCCTCCACCTGCCCTCT
  CCCCCAGTAAGGTCCACGGTGTGCGCTATGGACAGCCTGCGACCAGTGAGACTGCAGAAG
  TACCCTCAAGCAGCGGCGGTCCCTTAGTGACAGTGTCTACACCCCTCCACCAAGTGTCCC
  CCACGGGCCTGGAGCCCAGCCACAGCCTGCTGAGTACAGAAGCCAAGCTGGTCTCAGCAG
  CTGGGGGCCCCCTCCCCCCTGTCAGCACCCTGACAGCACTGCACAGCTTGGAGCAGACAT
  CCCCAGGCCTCAACCAGCAGCCCCAGAACCTCATCATGGCCTCACTTCCTGGGGTCATGA
  CCATCGGGCCTGGTGAGCCTGCCTCCCTGGGTCCTACGTTCACCAACACAGGTGCCTCCA
  CCCTGGTCATCGGCCTGGCCTCCACGCAGGCACAGAGTGTGCCGGTCATCAACAGCATGG
  GCAGCAGCCTGACCACCCTGCAGCCCGTCCAGTTCTCCCAGCCGCTGCACCCCTCCTACC
  AGCAGCCGCTCATGCCACCTGTGCAGAGCCATGTGACCCAGAGCCCCTTCATGGCCACCA
  TGGCTCAGCTGCAGAGCCCCCACGCCCTCTACAGCCACAAGCCCGAGGTGGCCCAGTACA
  CCCACACGGGCCTGCTCCCGCAGACTATGCTCATCACCGACACCACCAACCTGAGCGCCC
  TGGCCAGCCTCACGCCCACCAAGCAGGTCTTCACCTCAGACACTGAGGCCTCCAGTGAGT
  CCGGGCTTCACACGCCGGCATCTCAGGCCACCACCCTCCACGTCCCCAGCCAGGACCCTG
  CCGGCATCCAGCACCTGCAGCCGGCCCACCGGCTCAGCGCCAGCCCCACAGTGTCCTCCA
  GCAGCCTGGTGCTGTACCAGAGCTCAGACTCCAGCAATGGCCAGAGCCACCTGCTGCCAT
  CCAACCACAGCGTCATCGAGACCTTCATCTCCACCCAGATGGCCTCTTCCTCCCAGTAAC
  CACGGCACCTGGGCCCTGGGGCCTGTACTGCCTGCTTGGGGGGTGATGAGGGCAGCAGCC
  AGCCCTGCCTGGAGGACCTGAGCCTGCCGAGCAACCGTGGCCCTTCCTGGACAGCTGTGC
  CTCGCTCCCCACTCTGCTCTGATGCATCAGAAAGGGAGGGCTCTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000545 unedited
  GGGCTCAGATTTGTAAACGACTTATATAGGGCGGCCGCGAATCCGAGCCATGGTTTCTAA
  ACTGAGCCAGCTGCAGACGGAGCTCCTGGCGGCCCTGCTCGAGTCAGGGCTGAGCAAAGA
  GGCACTGATCCAGGCACTGGGTGAGCCGGGGCCCTACCTCCTGGCTGGAGAAGGCCCCCT
  GGACAAGGGGGAGTCCTGCGGCGGCGGTCGAGGGGAGCTGGCTGAGCTGCCCAATGGGCT
  GGGGGAGACTCGGGGCTCCGAGGACGAGACGGACGACGATGGGGAAGACTTCACGCCACC
  CATCCTCAAAGAGCTGGAGAACCTCAGCCCTGAGGAGGCGGCCCACCAGAAAGCCGTGGT
  GGAGACCCTTCTGCAGGAGGACCCGTGGCGTGTGGCGAAGATGGTCAAGTCCTACCTGCA
  GCAGCACAACATCCCACAGCGGGAGGTGGTCGATACCACTGGCCTCAACCAGTCCCACCT
  GTCCCAACACCTCAACAAGGGCACTCCCATGAAGACGCAGAAGCGGGCCGCCCTGTACAC
  CTGGTACGTCCGCAAGCAGCGAGAGGTGGCGCAGCAGTTCACCCATGCAGGGCAGGGAGG
  GCTGATTGAAGAGCCCACAGGTGATGAGCTACCAACCAAGAAGGNGCGGAGGAACCGTTT
  CAAGTGGGGCCCAGCATCCCAGCAGATCCTGTTCCAGGCCTATGAGAGGCAGAAGAACCC
  TAGCAAGGAGAGCGAGAGACGCTAGTGGAGGAGTGCAATAGGGCGGAATGCATCCAGAGA
  GGGGTGGTCCCATCACAGGCACAGGGGCTGGGGCTCAACCTCGTCACGGGAGTGCGTGTC
  TACAACTGGTTTGGCACCGGCGCAAAGAAGAAGCCTTTCCGCCCAAGCTGGCCCTGGAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000545 unedited
  CCACGTAGACTGCGCCTCGCAAGCTAGGTACAGCACCGTGGATAGGGTTCACAAGGTACT
  CACATCGGCATCGGTGTACGAATCAANAGCCCTCCCTTTCTGATGCATCAGAGCAGAGTG
  GGGAGCGAGGCACAGCTGTCCAGGAAGGGCCACGGTTGCTCGGCAGGCTCATGTCCTCCA
  GGCACGGCTGGCTGCTGCCCTCATCACCCCCCAAGCAGGCAGTACAGGCCCCAGGGCCCA
  CGTGCCGTGGTTACTGGGAGGAAGAGGCCATCTGGGTGGAGATGAAGGTCTCGATGACGC
  TGTGGTTGGATGGCAGCAAGTGGCTCTGGCCATTGCTGGAGTCTGACCTCTGGTACAGCA
  CCAGGCTGCTGGAGGACACTGTGGGGCTGGCGCTGAGCCGGTGGGCCGGCTGCAGGTGCT
  GGATGCCTGCAGGGTCCTGGCTGGCGACTTGAAGGGTGGTGGCCTGAGATGCCGGCCTGT
  GAAGCCCGGACTCACTGGAGGCCTCACTGTCTGACGTGAACACCTGCTTGGTGGGCGTGA
  GGCTGGCCATGGCGCTCAGGTTGGTGGTGTCTGTGATGAGCATACTCTGCGTGAGCAGGC
  ACCTGTGTGTGTACTGTGCCACCTCGGGCTTGTGGCTGTATAGGGCGTGGCGGCTCTGCA
  ACTGATCCATGGTGGCCATCGAAGGGGCTCTGGGGTCACATCGACTCTGCACATGTGGCA
  TTGACCGGCTCGCTGGTACGTATGGGTGCCAACCGCTGGGGAGAACTTGAACCGGGCTGC
  AGGGTGGATCAGGGCTGCTGCCCATGCTGGTTGATGACCCGGCACAACTCTGTGGCTTGC
  CTTGAAGGGCCAGAACCCATGACCCATGGGTGGAAGGCCACCGGGGGTGGTTGGAAACGT
  CAGGACCCAAGGGGAGGCCAAGCTTCACCAGCCCCCCATGGTGCCATAA
  
• Restriction Sites: Please inquire
• ACCN: NM_000545
• Insert Size: 2100 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000545.3, NP_000536.3
• RefSeq Size: 3249 bp
• RefSeq ORF: 1896 bp
• Locus ID: 6927
• Cytogenetics: 12q24.31
• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: Maturity onset diabetes of the young
• Gene Summary: 'The protein encoded by this gene is a transcription factor required for the expression of several liver-specific genes. The encoded protein functions as a homodimer and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Defects in this gene are a cause of maturity onset diabetes of the young type 3 (MODY3) and also can result in the appearance of hepatic adenomas. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]'
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.