CYP24A1 (NM_000782) Human Untagged Clone

CAT#: SC300131

CYP24A1 (untagged)-Human cytochrome P450, family 24, subfamily A, polypeptide 1 (CYP24A1), nuclear gene encoding mitochondrial protein, transcript variant 1


  "NM_000782" in other vectors (4)

Reconstitution Protocol

USD 870.00

2 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol CYP24A1
Synonyms CP24; CYP24; HCAI; HCINF1; P450-CC24
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_000782 edited
GCCCTTAAGCCCCGACTCCCACTAGCACCTCGTACCAACCTCGCCCCACCCCATCCTCCT
GCCTTCCCGCGCTCCGGTGTCCCCCGCTGCCATGAGCTCCCCCATCAGCAAGAGCCGCTC
GCTTGCCGCCTTCCTGCAGCAGCTGCGCAGTCCGAGGCAGCCCCCGAGACTGGTGACATC
TACGGCGTACACGTCCCCTCAGCCGCGAGAGGTGCCAGTCTGCCCGCTGACAGCTGGTGG
CGAGACTCAGAACGCGGCCGCCCTGCCGGGCCCCACCAGCTGGCCACTGCTGGGCAGCCT
GCTGCAGATTCTCTGGAAAGGGGGTCTCAAGAAACAGCACGACACCCTGGTGGAGTACCA
CAAGAAGTATGGCAAGATTTTCCGCATGAAGTTGGGTTCCTTTGAGTCGGTGCACCTGGG
CTCGCCATGCCTGCTGGAAGCGCTGTACCGCACCGAGAGCGCGTACCCGCAGCGGCTGGA
GATCAAACCGTGGAAGGCCTATCGCGACTACCGCAAAGAAGGCTACGGGCTGCTGATCCT
GGAAGGGGAAGACTGGCAGCGGGTCCGGAGTGCCTTTCAAAAGAAACTAATGAAACCAGG
GGAAGTGATGAAGCTGGACAACAAAATCAATGAGGTCTTGGCCCGATTTTATGGGCAGAA
TAGATGAGCTCTGTGATGAAAGAGGCCACGTCGAAGACTTGTACAGCGAACTGAACAAAT
GGTCGTTTGAAAGTATCTGCCTCGTGTTGTATGAGAAGAGATTTGGGCTTCTCCAGAAGA
ATGCAGGGGATGAAGCTGTGAACTTCATCATGGCCATCAAAACAATGATGAGCACGTTTG
GGAGGATGATGGTCACTCCAGTCGAGCTGCACAAGAGCCTCAACACCAAGGTCTGGCAGG
ACCACACTCTGGCCTGGGACACCATTTTCAAATCAGTCAAAGCTTGTATCGACAACCGGT
TAGAGAAGTATTCTCAGCAGCCTAGTGCAGATTTCCTTTGTGACATTTATCACCAGAATC
GGCTTTCAAAGAAAGAATTGTATGCTGCTGTCACAGAGCTCCAGCTGGCTGCGGTGGAAA
CGACAGCAAACAGTCTAATGTGGATTCTCTACAATTTATCCCGTAATCCCCAAGTGCAAC
AAAAGCTTCTTAAGGAAATTCAAAGTGTATTACCTGAGAATCAGGTGCCACGGGCAGAAG
ATTTGAGGAATATGCCGTATTTAAAAGCCTGTCTGAAAGAATCTATGAGGCTTACGCCGA
GTGTACCATTTACAACTCGGACTCTTGACAAGGCAACAGTTCTGGGTGAATATGCTTTAC
CCAAAGGAACAGTGCTCATGCTAAATACCCAGGTGTTGGGATCCAGTGAAGACAATTTTG
AAGATTCAAGTCAGTTTAGACCTGAACGTTGGCTTCAGGAGAAGGAAAAAATTAATCCTT
TTGCGCATCTTCCATTTGGCGTTGGAAAAAGAATGTGCATTGGTCGCCGATTAGCAGAGC
TTCAACTGCATTTGGCTCTTTGTTGGATTGTCCGCAAATACGACATCCAGGCCACAGACA
ATGAGCCTGTTGAGATGCTACACTCAGGCACCCTGGTGCCCAGCCGGGAACTCCCCATCG
CGTTTTGCCAGCGATAATACGCCTCAGATGGTGGTATTTGCTAACATCATATCCAACTCA
GGGAAGCGGACTGAGTGCTGGGATCCAAGGCATTCT
Restriction Sites Please inquire     
ACCN NM_000782
Insert Size 1700 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000782.3, NP_000773.2
RefSeq Size 3295 bp
RefSeq ORF 1545 bp
Locus ID 1591
Cytogenetics 20q13.2
Protein Families Druggable Genome
Gene Summary 'This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (1) encodes the longer isoform (1).

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.