CYP24A1 (NM_000782) Human Untagged Clone

CAT#: SC300131

CYP24A1 (untagged)-Human cytochrome P450, family 24, subfamily A, polypeptide 1 (CYP24A1), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CYP24A1
• Synonyms: CP24; CYP24; HCAI; HCINF1; P450-CC24
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000782 edited
  GCCCTTAAGCCCCGACTCCCACTAGCACCTCGTACCAACCTCGCCCCACCCCATCCTCCT
  GCCTTCCCGCGCTCCGGTGTCCCCCGCTGCCATGAGCTCCCCCATCAGCAAGAGCCGCTC
  GCTTGCCGCCTTCCTGCAGCAGCTGCGCAGTCCGAGGCAGCCCCCGAGACTGGTGACATC
  TACGGCGTACACGTCCCCTCAGCCGCGAGAGGTGCCAGTCTGCCCGCTGACAGCTGGTGG
  CGAGACTCAGAACGCGGCCGCCCTGCCGGGCCCCACCAGCTGGCCACTGCTGGGCAGCCT
  GCTGCAGATTCTCTGGAAAGGGGGTCTCAAGAAACAGCACGACACCCTGGTGGAGTACCA
  CAAGAAGTATGGCAAGATTTTCCGCATGAAGTTGGGTTCCTTTGAGTCGGTGCACCTGGG
  CTCGCCATGCCTGCTGGAAGCGCTGTACCGCACCGAGAGCGCGTACCCGCAGCGGCTGGA
  GATCAAACCGTGGAAGGCCTATCGCGACTACCGCAAAGAAGGCTACGGGCTGCTGATCCT
  GGAAGGGGAAGACTGGCAGCGGGTCCGGAGTGCCTTTCAAAAGAAACTAATGAAACCAGG
  GGAAGTGATGAAGCTGGACAACAAAATCAATGAGGTCTTGGCCCGATTTTATGGGCAGAA
  TAGATGAGCTCTGTGATGAAAGAGGCCACGTCGAAGACTTGTACAGCGAACTGAACAAAT
  GGTCGTTTGAAAGTATCTGCCTCGTGTTGTATGAGAAGAGATTTGGGCTTCTCCAGAAGA
  ATGCAGGGGATGAAGCTGTGAACTTCATCATGGCCATCAAAACAATGATGAGCACGTTTG
  GGAGGATGATGGTCACTCCAGTCGAGCTGCACAAGAGCCTCAACACCAAGGTCTGGCAGG
  ACCACACTCTGGCCTGGGACACCATTTTCAAATCAGTCAAAGCTTGTATCGACAACCGGT
  TAGAGAAGTATTCTCAGCAGCCTAGTGCAGATTTCCTTTGTGACATTTATCACCAGAATC
  GGCTTTCAAAGAAAGAATTGTATGCTGCTGTCACAGAGCTCCAGCTGGCTGCGGTGGAAA
  CGACAGCAAACAGTCTAATGTGGATTCTCTACAATTTATCCCGTAATCCCCAAGTGCAAC
  AAAAGCTTCTTAAGGAAATTCAAAGTGTATTACCTGAGAATCAGGTGCCACGGGCAGAAG
  ATTTGAGGAATATGCCGTATTTAAAAGCCTGTCTGAAAGAATCTATGAGGCTTACGCCGA
  GTGTACCATTTACAACTCGGACTCTTGACAAGGCAACAGTTCTGGGTGAATATGCTTTAC
  CCAAAGGAACAGTGCTCATGCTAAATACCCAGGTGTTGGGATCCAGTGAAGACAATTTTG
  AAGATTCAAGTCAGTTTAGACCTGAACGTTGGCTTCAGGAGAAGGAAAAAATTAATCCTT
  TTGCGCATCTTCCATTTGGCGTTGGAAAAAGAATGTGCATTGGTCGCCGATTAGCAGAGC
  TTCAACTGCATTTGGCTCTTTGTTGGATTGTCCGCAAATACGACATCCAGGCCACAGACA
  ATGAGCCTGTTGAGATGCTACACTCAGGCACCCTGGTGCCCAGCCGGGAACTCCCCATCG
  CGTTTTGCCAGCGATAATACGCCTCAGATGGTGGTATTTGCTAACATCATATCCAACTCA
  GGGAAGCGGACTGAGTGCTGGGATCCAAGGCATTCT
  
• Restriction Sites: Please inquire
• ACCN: NM_000782
• Insert Size: 1700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000782.3, NP_000773.2
• RefSeq Size: 3295 bp
• RefSeq ORF: 1545 bp
• Locus ID: 1591
• Cytogenetics: 20q13.2
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the longer isoform (1).