SREBP1 (SREBF1) (NM_001005291) Human Untagged Clone

CAT#: SC300896

SREBF1 (untagged)-Human sterol regulatory element binding transcription factor 1 (SREBF1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SREBF1
• Synonyms: bHLHd1; SREBP1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC300896 sequence for NM_001005291 edited (data generated by NextGen Sequencing)
  
  
  ATGGACGAGCCACCCTTCAGCGAGGCGGCTTTGGAGCAGGCGCTGGGCGAGCCGTGCGATCTGGACGCGG
  CGCTGCTGACCGACATCGAAGGTGAAGTCGGCGCGGGGAGGGGTAGGGCCAACGGCCTGGACGCCCCAAG
  GGCGGGCGCAGATCGCGGAGCCATGGATTGCACTTTCGAAGACATGCTTCAGCTTATCAACAACCAAGAC
  AGTGACTTCCCTGGCCTATTTGACCCACCCTATGCTGGGAGTGGGGCAGGGGGCACAGACCCTGCCAGCC
  CCGATACCAGCTCCCCAGGCAGCTTGTCTCCACCTCCTGCCACATTGAGCTCCTCTCTTGAAGCCTTCCT
  GAGCGGGCCGCAGGCAGCGCCCTCACCCCTGTCCCCTCCCCAGCCTGCACCCACTCCATTGAAGATGTAC
  CCGTCCATGCCCGCTTTCTCCCCTGGGCCTGGTATCAAGGAAGAGTCAGTGCCACTGAGCATCCTGCAGA
  CCCCCACCCCACAGCCCCTGCCAGGGGCCCTCCTGCCACAGAGCTTCCCAGCCCCAGCCCCACCGCAGTT
  CAGCTCCACCCCTGTGTTAGGCTACCCCAGCCCTCCGGGAGGCTTCTCTACAGGAAGCCCTCCCGGGAAC
  ACCCAGCAGCCGCTGCCTGGCCTGCCACTGGCTTCCCCGCCAGGGGTCCCGCCCGTCTCCTTGCACACCC
  AGGTCCAGAGTGTGGTCCCCCAGCAGCTACTGACAGTCACAGCTGCCCCCACGGCAGCCCCTGTAACGAC
  CACTGTGACCTCGCAGATCCAGCAGGTCCCGGTCCTGCTGCAGCCCCACTTCATCAAGGCAGACTCGCTG
  CTTCTGACAGCCATGAAGACAGACGGAGCCACTGTGAAGGCGGCAGGTCTCAGTCCCCTGGTCTCTGGCA
  CCACTGTGCAGACAGGGCCTTTGCCGACCCTGGTGAGTGGCGGAACCATCTTGGCAACAGTCCCACTGGT
  CGTAGATGCGGAGAAGCTGCCTATCAGCCGGCTCGCAGCTGGCAGCAAGGCCCCGGCCTCTGCCCAGAGC
  CGTGGAGAGAAGCGCACAGCCCACAACGCCATTGAGAAGCGCTACCGCTCCTCCATCAATGACAAAATCA
  TTGAGCTCAAGGATCTGGTGGTGGGCACTGAGGCAAAGCTGAATAAATCTGCTGTCTTGCGCAAGGCCAT
  CGACTACATTCGCTTTCTGCAACACAGCAACCAGAAACTCAAGCAGGAGAACCTAAGTCTGCGCACTGCT
  GTCCACAAAAGCAAATCTCTGAAGGATCTGGTGTCGGCCTGTGGCAGTGGAGGGAACACAGACGTGCTCA
  TGGAGGGCGTGAAGACTGAGGTGGAGGACACACTGACCCCACCCCCCTCGGATGCTGGCTCACCTTTCCA
  GAGCAGCCCCTTGTCCCTTGGCAGCAGGGGCAGTGGCAGCGGTGGCAGTGGCAGTGACTCGGAGCCTGAC
  AGCCCAGTCTTTGAGGACAGCAAGGCAAAGCCAGAGCAGCGGCCGTCTCTGCACAGCCGGGGCATGCTGG
  ACCGCTCCCGCCTGGCCCTGTGCACGCTCGTCTTCCTCTGCCTGTCCTGCAACCCCTTGGCCTCCTTGCT
  GGGGGCCCGGGGGCTTCCCAGCCCCTCAGATACCACCAGCGTCTACCATAGCCCTGGGCGCAACGTGCTG
  GGCACCGAGAGCAGAGATGGCCCTGGCTGGGCCCAGTGGCTGCTGCCCCCAGTGGTCTGGCTGCTCAATG
  GGCTGTTGGTGCTCGTCTCCTTGGTGCTTCTCTTTGTCTACGGTGAGCCAGTCACACGGCCCCACTCAGG
  CCCCGCCGTGTACTTCTGGAGGCATCGCAAGCAGGCTGACCTGGACCTGGCCCGGGGAGACTTTGCCCAG
  GCTGCCCAGCAGCTGTGGCTGGCCCTGCGGGCACTGGGCCGGCCCCTGCCCACCTCCCACCTGGACCTGG
  CTTGTAGCCTCCTCTGGAACCTCATCCGTCACCTGCTGCAGCGTCTCTGGGTGGGCCGCTGGCTGGCAGG
  CCGGGCAGGGGGCCTGCAGCAGGACTGTGCTCTGCGAGTGGATGCTAGCGCCAGCGCCCGAGACGCAGCC
  CTGGTCTACCATAAGCTGCACCAGCTGCACACCATGGGGAAGCACACAGGCGGGCACCTCACTGCCACCA
  ACCTGGCGCTGAGTGCCCTGAACCTGGCAGAGTGTGCAGGGGATGCCGTGTCTGTGGCGACGCTGGCCGA
  GATCTATGTGGCGGCTGCATTGAGAGTGAAGACCAGTCTCCCACGGGCCTTGCATTTTCTGACACGCTTC
  TTCCTGAGCAGTGCCCGCCAGGCCTGCCTGGCACAGAGTGGCTCAGTGCCTCCTGCCATGCAGTGGCTCT
  GCCACCCCGTGGGCCACCGTTTCTTCGTGGATGGGGACTGGTCCGTGCTCAGTACCCCATGGGAGAGCCT
  GTACAGCTTGGCCGGGAACCCAGTGGACCCCCTGGCCCAGGTGACTCAGCTATTCCGGGAACATCTCTTA
  GAGCGAGCACTGAACTGTGTGACCCAGCCCAACCCCAGCCCTGGGTCAGCTGATGGGGACAAGGAATTCT
  TGGATGCCCTCGGGTACCTGCAGCTGCTGAACAGCTGTTCTGATGCTGCGGGGGCTCCTGCCTACAGCTT
  CTCCATCAGTTCCAGCATGGCCACCACCACCGGCGTAGACCCGGTGGCCAAGTGGTGGGCCTCTCTGACA
  GCTGTGGTGATCCACTGGCTGCGGCGGGATGAGGAGGCGGCTGAGCGGCTGTGCCCGCTGGTGGAGCACC
  TGCCCCGGGTGCTGCAGGAGTCTGAGAGACCCCTGCCCAGGGCAGCTCTGCACTCCTTCAAGGCTGCCCG
  GGCCCTGCTGGGCTGTGCCAAGGCAGAGTCTGGTCCAGCCAGCCTGACCATCTGTGAGAAGGCCAGTGGG
  TACCTGCAGGACAGCCTGGCTACCACACCAGCCAGCAGCTCCATTGACAAGGCCGTGCAGCTGTTCCTGT
  GTGACCTGCTTCTTGTGGTGCGCACCAGCCTGTGGCGGCAGCAGCAGCCCCCGGCCCCGGCCCCAGCAGC
  CCAGGGCACCAGCAGCAGGCCCCAGGCTTCCGCCCTTGAGCTGCGTGGCTTCCAACGGGACCTGAGCAGC
  CTGAGGCGGCTGGCACAGAGCTTCCGGCCCGCCATGCGGAGGGTGTTCCTACATGAGGCCACGGCCCGGC
  TGATGGCGGGGGCCAGCCCCACACGGACACACCAGCTCCTCGACCGCAGTCTGAGGCGGCGGGCAGGCCC
  CGGTGGCAAAGGAGGCGCGGTGGCGGAGCTGGAGCCGCGGCCCACGCGGCGGGAGCACGCGGAGGCCTTG
  CTGCTGGCCTCCTGCTACCTGCCCCCCGGCTTCCTGTCGGCGCCCGGGCAGCGCGTGGGCATGCTGGCTG
  AGGCGGCGCGCACACTCGAGAAGCTTGGCGATCGCCGGCTGCTGCACGACTGTCAGCAGATGCTCATGCG
  CCTGGGCGGTGGGACCACTGTCACTTCCAGCTAG
  
  Clone variation with respect to NM_001005291.2
  1007:g=>a 2591:t=>c
• Restriction Sites: Please inquire
• ACCN: NM_001005291
• Insert Size: 4300 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to contain 2 SNPs compared with NM_001005291.1.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005291.1, NP_001005291.1
• RefSeq Size: 5012 bp
• RefSeq ORF: 3534 bp
• Locus ID: 6720
• Cytogenetics: 17p11.2
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Insulin signaling pathway
• Gene Summary: 'This gene encodes a basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a motif that is found in the promoter of the low density lipoprotein receptor gene and other genes involved in sterol biosynthesis. The encoded protein is synthesized as a precursor that is initially attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription. This cleaveage is inhibited by sterols. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternative promoter usage and splicing result in multiple transcript variants, including SREBP-1a and SREBP-1c, which correspond to RefSeq transcript variants 2 and 3, respectively. [provided by RefSeq, Nov 2017]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.