RYK (NM_001005861) Human Untagged Clone

CAT#: SC301044

RYK (untagged)-Human RYK receptor-like tyrosine kinase (RYK), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RYK
• Synonyms: D3S3195; JTK5; JTK5A; RYK1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001005861 edited
  ATGCGTGGGGCGGCGCGGCTGGGGCGGCCGGGCCGGAGTTGCCTCCCGGGGGCCCGCGGC
  CTGAGGGCCCCGCCGCCGCCGCCGCTGCTGCTTCTGCTTGCGCTGTTGCCGCTGCTGCCC
  GCGCCTGGCGCTGCCGCCGCCCCCGCCCCGCGGCCCCCGGAGCTGCAGTCGGCTTCCGCG
  GGGCCCAGCGTGAGTCTCTACCTGAGCGAGGACGAGGTGCGCCGGCTGATCGGTCTTGAT
  GCAGAACTTTATTATGTGAGAAATGACCTTATTAGTCACTACGCTCTATCCTTTAGTCTG
  TTAGTACCCAGTGAGACAAATTTCCTGCACTTCACCTGGCATGCGAAGTCCAAGGTTGAA
  TATAAGCTGGGATTCCAAGTGGACAATGTTTTGGCAATGGATATGCCCCAGGTCAACATT
  TCTGTTCAGGGGGAAGTTCCACGCACTTTATCAGTGTTTCGGGTAGAGCTTTCCTGTACT
  GGCAAAGTAGATTCTGAAGTTATGATACTAATGCAGCTCAACTTGACAGTAAATTCTTCA
  AAAAATTTTACCGTCTTAAATTTTAAACGAAGGAAAATGTGCTACAAAAAACTTGAAGAA
  GTAAAAACTTCAGCCTTGGACAAAAACACTAGCAGAACTATTTATGATCCTGTACATGCA
  GCTCCAACCACTTCTACGCGTGTGTTTTATATTAGTGTAGGGGTTTGTTGTGCAGTAATA
  TTTCTCGTAGCAATAATATTAGCTGTTTTGCACCTTCATAGTATGAAAAGGATTGAACTG
  GATGACAGCATTAGTGCCAGCAGTAGTTCCCAAGGGCTGTCTCAGCCATCCACCCAGACG
  ACTCAGTATCTGAGAGCAGACACGCCCAACAATGCAACTCCTATCACCAGCTCCTTAGGT
  TATCCTACCTTGCGGATAGAGAAGAACGACTTGAGAAGTGTCACTCTTTTGGAGGCCAAA
  GGCAAGGTGAAGGATATAGCAATATCCAGAGAGAGGATAACTCTAAAAGATGTACTCCAA
  GAAGGTACTTTTGGGCGTATTTTCCATGGGATTTTAATAGATGAAAAAGATCCAAATAAA
  GAAAAACAAGCATTTGTCAAAACAGTTAAAGATCAAGCTTCTGAAATTCAGGTGACAATG
  ATGCTCACTGAAAGTTGTAAGCTGCGAGGTCTTCATCACAGAAATCTTCTTCCTATTACT
  CATGTGTGTATAGAAGAAGGAGAAAAGCCCATGGTGATATTGCCTTACATGAATTGGGGG
  AATCTTAAATTGTTTTTACGACAGTGCAAGTTAGTAGAGGCCAATAATCCACAGGCAATT
  TCTCAGCAAGACCTGGTACACATGGCTATTCAGATTGCCTGTGGAATGAGCTACCTGGCC
  AGAAGGGAAGTCATCCACAAAGACCTGGCTGCCAGGAACTGTGTCATTGATGACACACTT
  CAAGTTAAGATCACAGACAATGCCCTCTCCAGAGACTTGTTCCCCATGGACTATCACTGT
  CTGGGGGACAATGAAAACAGGCCAGTTCGTTGGATGGCTCTTGAAAGTCTGGTTAATAAC
  GAGTTCTCTAGCGCTAGTGATGTGTGGGCCTTTGGAGTGACGCTGTGGGAACTCATGACT
  CTGGGCCAGACTCCCTACGTGGACATTGACCCCTTCGAGATGGCCGCATACCTGAAAGAT
  GGTTACCGAATAGCCCAGCCAATCAACTGTCCTGATGAATTATTTGCTGTGATGGCCTGT
  TGCTGGGCCTTAGATCCAGAGGAGAGGCCCAAGTTTCAGCAGCTGGTACAGTGCCTAACA
  GAGTTTCATGCAGCCCTGGGGGCCTACGTCTGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001005861 unedited
  GCGGCACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAATATAAAACAAATCCTTT
  AATTTTTCAAGTGTTTAAAAAACAATTTTATACTTAAGCCAGCCTTGAAGATAAGCACAA
  AATTTACCAGTTTACATTTAAAAAACAAACAAAAAACGACAACAACTCAAGCACCCGCTC
  TGTGCATAGCACTATTCTAGGTGCAATAAAAGGGAATCTTAACCTTAGAAATATGAGTTC
  ACTTTCTGGAATTGTATTATCTCCTTTTCCAGAGAGTAAAAATAAATAAAATCACCATTG
  TTTACTACAGATCTGCCCCAAACCACATCTGGTTCACAGAAAGGCTAATTTCTGCCAAAT
  TAAAGATGTAATGAACTCAGTTCCTGCTTTCCCAAAAACACGAAAGCAGAATTCCTTTTC
  ACTGAAAAAAATAAACAGTTTTCCATGCAAGGGCAGTTTGCTTCTAATAAGTATTTTTAA
  AAAAATTTTTTTTTCCTCTAGCTTTTCTTTAAATTTTCTTCCTCTAATATTGCCTTTTCT
  TGTACAAGGCAGACCAGGTATCTTTTTATGCTGTTTTTCCTTTACTAAGAAAAGCATTGC
  ATCTTGAAGACAAACCATTTCCCACAGTAGCGATAAAAAATAACACTAAAAAAACTTTAA
  AGGTGAGTCACTTACATCACCTTGATGAAGTAAAAAAATAAAAAGCAGCTGGCACTAAGA
  TAGCTAATTCCAAATATCTGCGTCTAAACAATCTTAAAAACTGTTTATTTATTTTGTAAG
  AAGTACCCCTAGCCCAAATAAGATGTTTTAAAAAATTTGCCAAAAAATTACAAAACCCTT
  AAGGCCACCTATAAAGTGATTCTAAAATTGCCCCATATTAAGCTGCCTCAAAGTCAGATC
  AAAAATTCTAAGGCCGAGTCTGAAGAAATGGCTTTGTGCGCTGGGTAAACN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001005861
• Insert Size: 3090 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005861.1, NP_001005861.1
• RefSeq Size: 2942 bp
• RefSeq ORF: 1833 bp
• Locus ID: 6259
• Cytogenetics: 3q22.2
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Gene Summary: The protein encoded by this gene is an atypical member of the family of growth factor receptor protein tyrosine kinases, differing from other members at a number of conserved residues in the activation and nucleotide binding domains. This gene product belongs to a subfamily whose members do not appear to be regulated by phosphorylation in the activation segment. It has been suggested that mediation of biological activity by recruitment of a signaling-competent auxiliary protein may occur through an as yet uncharacterized mechanism. The encoded protein has a leucine-rich extracellular domain with a WIF-type Wnt binding region, a single transmembrane domain, and an intracellular tyrosine kinase domain. This protein is involved in stimulating Wnt signaling pathways such as the regulation of axon pathfinding. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2012]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).