Uromucoid (UMOD) (NM_001008389) Human Untagged Clone

CAT#: SC301282

UMOD (untagged)-Human uromodulin (UMOD), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UMOD
• Synonyms: ADMCKD2; FJHN; HNFJ; HNFJ1; MCKD2; THGP; THP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001008389, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGGCAGCCATCTCTGACTTGGATGCTGATGGTGGTGGTGGCCTCTTGGTTCATCACAACTGCAGCCA
  CTGACACCTCAGAAGCAAGATGGTGCTCTGAATGTCACAGCAATGCCACCTGCACGGAGGATGAGGCCGT
  TACGACGTGCACCTGTCAGGAGGGCTTCACCGGCGATGGCCTGACCTGCGTGGACCTGGATGAGTGCGCC
  ATTCCTGGAGCTCACAACTGCTCCGCCAACAGCAGCTGCGTAAACACGCCAGGCTCCTTCTCCTGCGTCT
  GCCCCGAAGGCTTCCGCCTGTCGCCCGGTCTCGGCTGCACAGACGTGGATGAGTGCGCTGAGCCTGGGCT
  TAGCCACTGCCACGCCCTGGCCACATGTGTCAATGTGGTGGGCAGCTACTTGTGCGTATGCCCCGCGGGC
  TACCGGGGGGATGGATGGCACTGTGAGTGCTCCCCGGGCTCCTGCGGGCCGGGGTTGGACTGCGTGCCCG
  AGGGCGACGCGCTCGTGTGCGCGGATCCGTGCCAGGCGCACCGCACCCTGGACGAGTACTGGCGCAGCAC
  CGAGTACGGGGAGGGCTACGCCTGCGACACGGACCTGCGCGGCTGGTACCGCTTCGTGGGCCAGGGCGGT
  GCGCGCATGGCCGAGACCTGCGTGCCAGTCCTGCGCTGCAACACGGCCGCCCCCATGTGGCTCAATGGCA
  CGCATCCGTCCAGCGACGAGGGCATCGTGAGCCGCAAGGCCTGCGCGCACTGGAGCGGCCACTGCTGCCT
  GTGGGATGCGTCCGTCCAGGTGAAGGCCTGTGCCGGCGGCTACTACGTCTACAACCTGACAGCGCCCCCC
  GAGTGTCACCTGGCGTACTGCACAGACCCCAGCTCCGTGGAGGGGACGTGTGAGGAGTGCAGTATAGACG
  AGGACTGCAAATCGAATAATGGCAGATGGCACTGCCAGTGCAAACAGGACTTCAACATCACTGATATCTC
  CCTCCTGGAGCACAGGCTGGAATGTGGGGCCAATGACATGAAGGTGTCGCTGGGCAAGTGCCAGCTGAAG
  AGTCTGGGCTTCGACAAGGTCTTCATGTACCTGAGTGACAGCCGGTGCTCGGGCTTCAATGACAGAGACA
  ACCGGGACTGGGTGTCTGTAGTGACCCCAGCCCGGGATGGCCCCTGTGGGACAGTGTTGACGAGGAATGA
  AACCCATGCCACTTACAGCAACACCCTCTACCTGGCAGATGAGATCATCATCCGTGACCTCAACATCAAA
  ATCAACTTTGCATGCTCCTACCCCCTGGACATGAAAGTCAGCCTGAAGACCGCCCTACAGCCAATGGTCA
  GTGCTCTAAACATCAGAGTGGGCGGGACCGGCATGTTCACCGTGCGGATGGCGCTCTTCCAGACCCCTTC
  CTACACGCAGCCCTACCAAGGCTCCTCCGTGACACTGTCCACTGAGGCTTTTCTCTACGTGGGCACCATG
  TTGGATGGGGGCGACCTGTCCCGATTTGCACTGCTCATGACCAACTGCTATGCCACACCCAGTAGCAATG
  CCACGGACCCCCTGAAGTACTTCATCATCCAGGACAGATGCCCACACACTAGAGACTCAACTATCCAAGT
  GGTGGAGAATGGGGAGTCCTCCCAGGGCCGATTTTCCGTCCAGATGTTCCGGTTTGCTGGAAACTATGAC
  CTAGTCTACCTGCACTGTGAAGTCTATCTCTGTGACACCATGAATGAAAAGTGCAAGCCTACCTGCTCTG
  GGACCAGATTCCGAAGTGGGAGTGTCATAGATCAATCCCGTGTCCTGAACTTGGGTCCCATCACACGGAA
  AGGTGTCCAGGCCACAGTCTCAAGGGCTTTTAGCAGCTTGGGGCTCCTGAAAGTCTGGCTGCCTCTGCTT
  CTCTCGGCCACCTTGACCCTGACTTTTCAGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001008389
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008389.2, NP_001008390.1
• RefSeq Size: 2427 bp
• RefSeq ORF: 1923 bp
• Locus ID: 7369
• Cytogenetics: 16p12.3
• Gene Summary: 'The protein encoded by this gene is the most abundant protein in mammalian urine under physiological conditions. Its excretion in urine follows proteolytic cleavage of the ectodomain of its glycosyl phosphatidylinosital-anchored counterpart that is situated on the luminal cell surface of the loop of Henle. This protein may act as a constitutive inhibitor of calcium crystallization in renal fluids. Excretion of this protein in urine may provide defense against urinary tract infections caused by uropathogenic bacteria. Defects in this gene are associated with the renal disorders medullary cystic kidney disease-2 (MCKD2), glomerulocystic kidney disease with hyperuricemia and isosthenuria (GCKDHI), and familial juvenile hyperuricemic nephropathy (FJHN). Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2013]'
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same isoform (a).