LHX9 (NM_001014434) Human Untagged Clone

CAT#: SC301932

LHX9 (untagged)-Human LIM homeobox 9 (LHX9), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LHX9
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001014434 edited
  ATGCTGAACGGTACCACTCTAGAGGCAGCCATGCTCTTTCACGGGATCTCCGGAGGCCAC
  ATCCAAGGCATCATGGAGGAGATGGAGCGCAGATCCAAGACTGAGGCCCGTCTGGCCAAA
  GGCGCCCAGCTCAACGGCCGCGACGCGGGCATGCCCCCGCTCAGCCCGGAGAAGCCCGCC
  CTGTGCGCCGGCTGCGGGGGCAAGATCTCGGACAGGTACTATCTGCTGGCTGTGGACAAA
  CAGTGGCATCTGAGATGCCTGAAGTGCTGTGAATGTAAGCTGGCCCTCGAGTCCGAGCTC
  ACCTGCTTTGCCAAGGACGGTAGCATTTACTGCAAGGAGGATTACTACAGAAGGTTCTCT
  GTGCAGAGATGTGCCCGCTGCCACCTTGGCATTTCCGCCTCGGAGATGGTCATGCGCGCC
  CGAGACTCTGTCTACCACCTGAGCTGCTTCACCTGCTCCACTTGCAACAAGACTCTGACC
  ACGGGCGACCATTTCGGCATGAAGGACAGCCTGGTGTACTGCCGCGCCCACTTCGAGACC
  CTCTTGCAAGGAGAGTATCCACCGCAGCTGAGCTACACGGAGCTGGCGGCCAAGAGCGGC
  GGCCTGGCCCTGCCTTACTTCAACGGTACGGGCACCGTGCAGAAAGGGCGGCCCCGGAAG
  CGGAAGAGCCCAGCGCTGGGAGTGGACATCGTCAATTACAACTCAGGTTGTAATGAGAAT
  GAGGCAGACCACTTGGACCGGGACCAGCAGCCTTATCCACCCTCGCAGAAGACCAAGCGC
  ATGCGAACCTCTTTCAAGCATCACCAGCTCCGGACCATGAAATCCTACTTTGCCATCAAC
  CACAACCCGGATGCCAAGGACCTCAAGCAGCTTGCCCAGAAAACAGGTCTGACCAAAAGA
  GTTTTGCAGGTTTGGTTCCAAAACGCACGAGCCAAATTCAGAAGGAACCTTTTGCGGCAG
  GAGAATGGGGGTGTTGATAAAGCTGACGGCACGTCGCTTCCGGCCCCGCCCTCAGCAGAC
  AGCGGAGCTCTCACTCCACCCGGCACTGCGACCACTTTAACAGACCTGACCAATCCCACT
  ATCACTGTAGTGACATCCGTGACCTCTAACATGGACAGCCACGAATCCGGAAGCCCCTCA
  CAAACTACCTTAACAAACCTTTTCTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001014434 unedited
  TTCAAGTTGTATACGACTCCTATAGGGCGGCCGCGAACCGGCACGAGGCTGAACGCAACC
  TCAGAGCGTTTCTCTGGGCACCGGCCCGCGTCTGTGCCCTCTGCCCACCCCTTCCTCCTC
  CCCCTCCCGATTTGGAAGCATAAACAGAAAATAAAAGACAGGTGGAGAGAGCTAAGGGTC
  AGAGGGGGACCCTCAGAGAGACGCAGGGACTGGCCCGCACCTCTGGCCGCCGCCTCCCGC
  CGAGAACCGAGTTCTAGTTGGGGAAACGTCTGACATCTGGACATCAAGTGCAACCACCAT
  TACGGCGTGATCCACTCCTTTTCCTCTAAGAATGCTGAACGGTACCACTCTAGAGGCAGC
  CATGCTCTTTCACGGGATCTCCGGAGGCCACATCCAAGGCATCATGGAGGAGATGGAGCG
  CAGATCCAAGACTGAGGCCCGTCTGGCCAAAGGCGCCCAGCTCAACGGCCGCGACGCGGG
  CATGCCCCCGCTCAGCCCGGAGAAGCCCGCCCTGTGCGCCGGCTGCGGGGGCAAGATCTC
  GGACAGGTACTATCTGCTGGCTGTGGACAAACAGTGGCATCTGAGATGCCTGAAGTGCTG
  TGAATGTAAGCTGGCCCTCGAGTCCGAGCTCACCTGCTTTGCCAAGGACGGTAGCATTTA
  CTGCAAGGAGGATTACTACAGAAAGTTCTCTGTGCAGAGATGTGCCCGCTGCCACCTTGG
  CATTTCCGCCTCGGAGATGGTCATGCGCGCCCGAGACTCTGTCTACCACCTGAGCTGCTT
  CACCTGCTCCACTTGCAACAAGACTCTGACCACGGGCGACCATTTCGGCATGAAGACAGC
  CTGTGTACTGCCGCGCCCACTTCGAGACCCTCTTGCAAGGAGAGTATCCACCGCAGCTGA
  GCTACACGGAGCTGGCGCCAAAAGCGGCGGCCT
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_001014434 unedited
  ATCATCTGCCGGGCGACCTTTCGGCATGAAGGACAGCCTGGTGTACTGCCGCGCCCACTT
  CGAGACCCTCTTGCAAGGAGAGTATCCACCGCAGCTGAGCTACACGGAGCTGGCGGCCAA
  GAGCGGCGGCCTGGCCCTGCCTTACTTCAACGGTACGGGCACCGTGCAGAAAGGGCGGCC
  CCGGAAGCGGAAGAGCCCAGCGCTGGGAGTGGACATCGTCAATTACAACTCAGGTTGTAA
  TGAGAATGAGGCAGACCACTTGGACCGGGACCAGCAGCCTTATCCACCCTCGCAGAAGAC
  CAAGCGCATGCGAACCTCTTTCAAGCATCACCAGCTCCGGACCATGAAATCCTACTTTGC
  CATCAACCACAACCCGGATGCCAAGGACCTCAAGCAGCTTGCCCAGAAAACAGGTCTGAC
  CAAAAGAGTTTTGCAGGTTTGGTTCCAAAACGCACGAGCCAAATTCAGAAGGAACCTTTT
  GCGGCAGGAGAATGGGGGTGTTGATAAAGCTGACGGCACGTCGCTTCCGGCCCCGCCCTC
  AGCAGACAGCGGAGCTCTCACTCCACCCGGCACTGCGACCACTTTAACAGACCTGACCAA
  TCCCACTATCACTGTAGTGACATCCGTGACCTCTAACATGGACAGCCACGAATCCGGAAG
  CCCCTCACAAACTACCTTAACAAACCTTTTCTAACATTGGTTTTTTTTTTTTTAGTTTTT
  AAATTCTTCCTCTTCTTTTTATTATTATTCTAATTATTATTATTTTATTATTTACAGACT
  TTTTTTTTTTCTTTCTAACCCACAAGATATTTGGGGAATAAAAAATAACAGCTTGGTGTG
  TAGCATCTGCAGCCACTTGGCAATGAGTTTACAGTAATTGTCTCCTTTAAGTGAATATTA
  TTTTTGTCTTACAAAGTGTTATTTTGGAATTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001014434
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001014434.1, NP_001014434.1
• RefSeq Size: 2078 bp
• RefSeq ORF: 1167 bp
• Locus ID: 56956
• Cytogenetics: 1q31.3
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene encodes a member of the LIM homeobox gene family of developmentally expressed transcription factors. The encoded protein contains a homeodomain and two cysteine-rich zinc-binding LIM domains involved in protein-protein interactions. The protein is highly similar to a mouse protein that causes gonadal agenesis when inactivated, suggesting a role in gonadal development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) differs in the 5' UTR and coding region compared to variant 1, resulting in a protein that maintains the reading frame but has a shorter N-terminus.