USP1 (NM_001017415) Human Untagged Clone

CAT#: SC302043

USP1 (untagged)-Human ubiquitin specific peptidase 1 (USP1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: USP1
• Synonyms: UBP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001017415, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCTGGTGTCATACCTAGTGAAAGTAATGGACTTTCAAGAGGTAGCCCTTCAAAGAAAAACAGACTTT
  CCTTAAAGTTTTTTCAGAAAAAGGAAACTAAGAGAGCTTTGGATTTCACAGATTCTCAAGAAAATGAAGA
  AAAAGCTTCTGAATATAGAGCATCTGAAATTGATCAAGTTGTTCCTGCAGCACAGTCTTCACCTATAAAC
  TGTGAGAAGAGAGAAAACTTGTTACCATTTGTGGGACTGAATAATCTCGGCAATACTTGCTATCTTAATA
  GTATACTTCAGGTATTATATTTTTGTCCCGGTTTTAAATCTGGAGTAAAGCACTTATTTAATATTATTTC
  AAGGAAGAAAGAAGCTCTAAAGGATGAAGCCAATCAAAAAGACAAGGGAAATTGCAAAGAAGATTCTTTG
  GCAAGTTATGAATTGATATGCAGTTTACAGTCCTTAATCATTTCGGTTGAACAGCTCCAGGCTAGTTTTC
  TCTTAAATCCAGAGAAATATACTGATGAACTTGCCACTCAGCCAAGGCGACTGCTTAACACACTGAGGGA
  ACTCAACCCTATGTATGAAGGATATCTACAGCATGATGCACAGGAAGTATTACAATGTATTTTGGGAAAC
  ATTCAAGAAACATGCCAACTCCTAAAAAAAGAAGAAGTAAAAAATGTGGCAGAATTACCTACTAAGGTAG
  AAGAAATACCTCATCCGAAAGAGGAAATGAATGGTATTAACAGCATAGAGATGGACAGTATGAGGCATTC
  TGAAGACTTTAAAGAGAAACTCCCAAAAGGAAATGGGAAAAGAAAAAGTGACACTGAATTTGGTAACATG
  AAGAAAAAAGTTAAATTATCCAAGGAACACCAGTCATTGGAAGAGAACCAGAGACAAACTAGATCAAAAA
  GAAAAGCTACAAGTGATACATTAGAGAGTCCTCCTAAAATAATTCCCAAGTATATTTCTGAAAATGAGAG
  TCCAAGACCCTCACAAAAGAAATCAAGAGTTAAAATAAATTGGTTAAAGTCTGCAACTAAGCAACCCAGC
  ATTCTTTCTAAATTTTGTAGTCTGGGAAAAATAACAACAAACCAAGGAGTCAAAGGACAATCTAAAGAAA
  ATGAATGTGATCCTGAAGAGGACTTGGGGAAGTGTGAAAGTGATAACACAACTAATGGTTGTGGACTTGA
  ATCTCCAGGAAATACTGTTACACCTGTAAATGTTAATGAAGTTAAACCCATAAACAAAGGTGAAGAACAA
  ATTGGTTTTGAGCTAGTGGAGAAATTATTTCAAGGTCAGCTGGTATTAAGGACGCGTTGCTTGGAATGTG
  AAAGTTTAACAGAAAGAAGAGAAGATTTTCAAGACATCAGTGTGCCAGTACAAGAAGATGAGCTTTCCAA
  AGTAGAGGAGAGTTCTGAAATTTCTCCAGAGCCAAAAACAGAAATGAAGACCCTGAGATGGGCAATTTCA
  CAATTTGCTTCAGTAGAAAGGATTGTAGGAGAAGATAAATATTTCTGTGAAAACTGCCATCATTATACTG
  AAGCTGAACGAAGTCTTTTGTTTGACAAAATGCCTGAAGTTATAACTATTCATTTGAAGTGCTTTGCTGC
  TAGTGGTTTGGAGTTTGATTGTTATGGTGGTGGACTTTCCAAGATCAACACTCCTTTATTGACACCTCTT
  AAATTGTCACTAGAAGAATGGAGCACAAAGCCAACTAACGACAGCTATGGATTATTTGCGGTTGTGATGC
  ATAGTGGCATTACAATTAGTAGTGGGCATTACACTGCTTCTGTTAAAGTCACTGACCTTAACAGTTTAGA
  ACTAGATAAAGGAAATTTTGTGGTTGACCAAATGTGTGAAATAGGTAAGCCAGAACCATTGAATGAGGAG
  GAAGCAAGGGGTGTGGTTGAGAATTATAATGATGAAGAAGTGTCAATTAGAGTTGGTGGAAATACACAGC
  CAAGTAAAGTTTTGAACAAAAAAAATGTAGAAGCTATTGGACTTCTTGGAGGACAAAAGAGCAAAGCAGA
  TTATGAGCTATACAACAAAGCCTCTAATCCTGATAAGGTTGCTAGTACAGCGTTTGCTGAAAATAGAAAT
  TCTGAGACTAGTGATACTACTGGGACCCATGAATCTGATAGAAACAAGGAATCCAGTGACCAAACAGGCA
  TTAATATTAGTGGATTTGAGAACAAAATTTCATACGTAGTGCAAAGCTTAAAGGAGTATGAGGGGAAGTG
  GTTGCTTTTTGATGATTCTGAAGTCAAAGTTACTGAAGAGAAGGACTTTCTGAATTCTCTTTCCCCTTCT
  ACATCTCCTACTTCTACTCCTTACTTGCTATTTTATAAGAAATTATAG
  
  
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001017415
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001017415.1, NP_001017415.1
• RefSeq Size: 3519 bp
• RefSeq ORF: 2358 bp
• Locus ID: 7398
• Cytogenetics: 1p31.3
• Protein Families: Protease
• Gene Summary: 'This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. The protein specifically deubiquitinates a protein in the Fanconi anemia (FA) DNA repair pathway. Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) uses an alternate upstream transcriptional start site and contains an alternate exon in the 5' UTR, compared to variant 1. Variants 1, 2 and 3 encode the same protein.