PRDM1 (NM_001198) Human Untagged Clone

CAT#: SC302988

PRDM1 (untagged)-Human PR domain containing 1, with ZNF domain (PRDM1), transcript variant 1


  "NM_001198" in other vectors (7)

Reconstitution Protocol

USD 1,670.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PRDM1
Synonyms BLIMP1; PRDI-BF1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_001198 edited
GACGCGGGGAGAATGTGGACTGGGTAGAGATGAACGAGACTTTTCTCAGATGTTGGATAT
TTGCTTGGAAAAACGTGTGGGTACGACCTTGGCTGCCCCCAAGTGTAACTCCAGCACTGT
GAGGTTTCAGGGATTGGCAGAGGGGACCAAGGGGACCATGAAAATGGACATGGAGGATGC
GGATATGACTCTGTGGACAGAGGCTGAGTTTGAAGAGAAGTGTACATACATTGTGAACGA
CCACCCCTGGGATTCTGGTGCTGATGGCGGTACTTCGGTTCAGGCGGAGGCATCCTTACC
AAGGAATCTGCTTTTCAAGTATGCCACCAACAGTGAAGAGGTTATTGGAGTGATGAGTAA
AGAATACATACCAAAGGGCACACGTTTTGGACCCCTAATAGGTGAAATCTACACCAATGA
CACAGTTCCTAAGAACGCCAACAGGAAATATTTTTGGAGGATCTATTCCAGAGGGGAGCT
TCACCACTTCATTGACGGCTTTAATGAAGAGAAAAGCAACTGGATGCGCTATGTGAATCC
AGCACACTCTCCCCGGGAGCAAAACCTGGCTGCGTGTCAGAACGGGATGAACATCTACTT
CTACACCATTAAGCCCATCCCTGCCAACCAGGAACTTCTTGTGTGGTATTGTCGGGACTT
TGCAGAAAGGCTTCACTACCCTTATCCCGGAGAGCTGACAATGATGAATCTCACACAAAC
ACAGAGCAGTCTAAAGCAACCGAGCACTGAGAAAAATGAACTCTGCCCAAAGAATGTCCC
AAAGAGAGAGTACAGCGTGAAAGAAATCCTAAAATTGGACTCCAACCCCTCCAAAGGAAA
GGACCTCTACCGTTCTAACATTTCACCCCTCACATCAGAAAAGGACCTCGATGACTTTAG
AAGACGTGGGAGCCCCGAAATGCCCTTCTACCCTCGGGTCGTTTACCCCATCCGGGCCCC
TCTGCCAGAAGACTTTTTGAAAGCTTCCCTGGCCTACGGGATCGAGAGACCCACGTACAT
CACTCGCTCCCCCATTCCATCCTCCACCACTCCAAGCCCCTCTGCAAGAAGCAGCCCCGA
CCAAAGCCTCAAGAGCTCCAGCCCTCACAGCAGCCCTGGGAATACGGTGTCCCCTGTGGG
CCCCGGCTCTCAAGAGCACCGGGACTCCTACGCTTACTTGAACGCGTCCTACGGCACGGA
AGGTTTGGGCTCCTACCCTGGCTACGCACCCCTGCCCCACCTCCCGCCAGCTTTCATCCC
CTCGTACAACGCTCACTACCCCAAGTTCCTCTTGCCCCCCTACGGCATGAATTGTAATGG
CCTGAGCGCTGTGAGCAGCATGAATGGCATCAACAACTTTGGCCTCTTCCCGAGGCTGTG
CCCTGTCTACAGCAATCTCCTCGGTGGGGGCAGCCTGCCCCACCCCATGCTCAACCCCAC
TTCTCTCCCGAGCTCGCTGCCCTCAGATGGAGCCCGGAGGTTGCTCCAGCCGGAGCATCC
CAGGGAGGTGCTTGTCCCGGCGCCCCACAGTGCCTTCTCCTTTACCGGGGCCGCCGCCAG
CATGAAGGACAAGGCCTGTAGCCCCACAAGCGGGTCTCCCACGGCGGGAACAGCCGCCAC
GGCAGAACATGTGGTGCAGCCCAAAGCTACCTCAGCAGCGATGGCAGCCCCCAGCAGCGA
CGAAGCCATGAATCTCATTAAAAACAAAAGAAACATGACCGGCTACAAGACCCTTCCCTA
CCCGCTGAAGAAGCAGAACGGCAAGATCAAGTACGAATGCAACGTTTGCGCCAAGACTTT
CGGCCAGCTCTCCAATCTGAAGGTCCACCTGAGAGTGCACAGTGGAGAACGGCCTTTCAA
ATGTCAGACTTGCAACAAGGGCTTTACTCAGCTCGCCCACCTGCAGAAACACTACCTGGT
ACACACGGGAGAAAAGCCACATGAATGCCAGGTCTGCCACAAGAGATTTAGCAGCACCAG
CAATCTCAAGACCCACCTGCGACTCCATTCTGGAGAGAAACCATACCAATGCAAGGTGTG
CCCTGCCAAGTTCACCCAGTTTGTGCACCTGAAACTGCACAAGCGTCTGCACACCCGGGA
GCGGCCCCACAAGTGCTCCCAGTGCCACAAGAACTACATCCATCTCTGTAGCCTCAAGGT
TCACCTGAAAGGGAACTGCGCTGCGGCCCCGGCGCCTGGGCTGCCCTTGGAAGATCTGAC
CCGAATCAATGAAGAAATCGAGAAGTTTGACATCAGTGACAATGCTGACCGGCTCGAGGA
CGTGGAGGATGACATCAGTGTGATCTCTGTAGTGGAGAAGGAAATTCTGGCCGTGGTCAG
AAAAGAGAAAGAAGAAACTGGCCTGAAAGTGTCTTTGCAAAGAAACATGGGGAATGGACT
CCTCTCCTCAGGGTGCAGCCTTTATGAGTCATCAGATCTACCCCTCATGAAGTTGCCTCC
CAGCAACCCACTACCTCTGGTACCTGTAAAGGTCAAACAAGAAACAGTTGAACCAATGGA
TCCTTAAGATTTTCAGAAAACACTTATTTTGTTTCTTAAGTTATGACTTGGTGAGTCAGG
GTGCCTGTAGGAAGTGGCTTGTACATAATCCCAGCTCTGCAAAGCTCTCTCGACAGCAAA
TGGTTTCCCCTCACCTCTGGAATTAAAGAAGGAACTCCAAAGTTACTGAAATCTCAGGGC
ATGAACAAGGCAAAGGCCATATATATATATATATATATATCTGTATACATATTATATATA
CTTATTTACACCTGTGTCTATATATTTGCCCCTGTGTATTTTGAATATTTGTGTGGACAT
GTTTGCATAGCCTTCCCATTACTAAGACTATTACCTAGTCATAATTATTTTTTCAATGAT
AATCCTTCATAATTTATTATACAATTTATCATTCAGAAAGCAATAATTAAAAAAGTTTAC
AATGACTGGAAAGATTCCTTGTAATTTGAGTATAAATGTATTTTTGTCTTGTGGCCATTC
TTTGTAGATAATTTCTGCACATCTGTATAAGTACCTAAGATTTAGTTAAACAAATATATG
ACTTCAGTCAACCTCTCTCTCTAATAATGGTTTGAAAATGAGGTTTGGGTAATTGCCAAT
GTTGGACAGTTGATGTGTTCATTCCTGGGATCCTATCATTTGAACAGCATTGTACATAAC
TTGGGGGTATGTGTGCAGGATTACCCAAGAATAACTTAAGTAGAAGAAACAAGAAAGGGA
ATCTTGTATATTTTTGTTGATAGTTCATGTTTTTCCCCCAGCCACAATTTTACCGGAAGG
GTGACAGGAAGGCTTTACCAACCTGTCTCTCCCTCCAAAAGAGCAGAATCCTCCCACCGC
CCTGCCCTCCCCACCGAGTCCTGTGGCCATTCAGAGCGGCCACATGACTTTTGCATCCAT
TGTATTATCAGAAAATGTGAAGAAGAAAAAAATGCCATGTTTTAAAACCACTGCGAAAAT
TTCCCCAAAGCATAGGTGGCTTTGTGTGTGTGCGATTTGGGGGCTTGAGTCTGGGTGGTG
TTTTGTTGTTGGTTTTTGTTGCTTTTTTTTTTTTTTTTTTTTTAATGTCAAAATTGCACA
AACATGGTGCTCTACCAGGAAGGATTCGAGGTAGATAGGCTCAGGCCACACTTTAAAAAC
AAACACACAAACAACAAAAAACGGGTATTCTAGTCATCTTGGGGTAAAAGCGGGTAATGA
ACATTCCTATCCCCAACACATCAATTGTATTTTTTCTGTAAAACTCAGATTTTCCTCAGT
ATTTGTGTTTTTACATTTTATGGTTAATTTAATGGAAGATGAAAGGGCATTGCAAAGTTG
TTCAACAACAGTTACCTCATTGAGTGTGTCCAGTAGTGCAGGAAATGATGTCTTATCTAA
TGATTTGCTTCTCTAGAGGAGAAACCGAGTAAATGTGCTCCAGCAAGATAGACTTTGTGT
TATTCTATCTTTTATTCTGCTAAGCCCAAAGATTACATGTTGGTGTTCAAAGTGTAGCAA
AAAATGATGTATATTTATAAATCTATTTATACCACTATATCATATGTATATATATTTATA
ACCACTTAAATTGTGAGCCAAGCCATGTAAAAGATCTACTTTTTCTAAGGGCAAAAAAAA
AAAAAAAAAAAAAAGAACACTCCTTTCTGAGACTTTGCTTAATACTTGGTGACCTCACAA
TCACGTCGGTATGATTGGGCACCCTTGCCTACTGTAAGAGACCCTAAAACCTTGGTGCAG
TGGTGGGGACCACAAAACAACCAGGGAGGAAGAGATACATCATTTTTTAGTATTAAGGAC
CATCTAAGACAGCTCTATTTTTTTTTTGCCACTTTATGATTATGTGGTCACACCCAAGTC
ACAGAAATAAAAAACTGACTTTACCGCTGCAATTTTTCTGTTTTCCTCCTTACTAAATAC
TGATACATTACTCCAATCTATTTTATAATTATATTTGACATTTTGTTCACATCAACTAAT
GTTCACCTGTAGAAGAGAACAAATTTCGAATAATCCAGGGAAACCCAAGAGCCTTACTGG
TCTTCTGTAACTTCCAAGACTGACAGCTTTTTATGTATCAGTGTTTGATAAACACAGTCC
TTAACTGAAGGTAAACCAAAGCATCACGTTGACATTAGACCAAATACTTTTGATTCCCAA
CTACTCGTTTGTTCTTTTTCTCCTTTTGTGCTTTCCCATAGTGAGAATTTTTATAAAGAC
TTCTTGCTTCTCTCACCATCCATCCTTCTCTTTTCTGCCTCTTACATGTGAATGTTGAGC
CCACAATCAACAGTGGTTTTATTTTTTCCTCTACTCAAAGTTAAAACTGACCAAAGTTAC
TGGCTTTTTACTTTGCTAGAACAACAAACTATCTTATGTTTACATACTGGTTTACAATGT
TATTTATGTGCAAATTGTCAAAATGTAAATTAAATATAAATGTTCATGCTTTACCAAAAA
AAAAAAAAAAAAA
>OriGene 5' read for NM_001198 unedited
TTTGTACGGTCTCCATTTGTATACGACTCATATAGGCGGCCGCAGATTCGCCCTTGACGC
GGGGAGAATGTGGACTGGGTAGAGATGAACGAGACTTTTCTCAGATGTTGGATATTTGCT
TGGAAAAACGTGTGGGTACGACCTTGGCTGCCCCCAAGTGTAACTCCAGCACTGTGAGGT
TTCAGGGATTGGCAGAGGGGACCAAGGGGACCATGAAAATGGACATGGAGGATGCGGATA
TGACTCTGTGGACAGAGGCTGAGTTTGAAGAGAAGTGTACATACATTGTGAACGACCACC
CCTGGGATTCTGGTGCTGATGGCGGTACTTCGGTTCAGGCGGAGGCATCCTTACCAAGGA
ATCTGCTTTTCAAGTATGCCACCAACAGTGAAGAGGTTATTGGAGTGATGAGTAAAGAAT
ACATACCAAAGGGCACACGTTTTGGACCCCTAATAGGTGAAATCTACACCAATGACACAG
TTCCTAAGAACGCCAACAGGAAATATTTTTGGAGGATCTATTCCAGAGGGGAGCTTCACC
ACTTCATTGACGGCTNTAATGAAGAGAAAAAGCACTGGATGCGCTATGTGAATCCAGCAC
ACTCTCCCCGGGAGCAAAACCTGNCTGCGTGTCAGAACGGGATGAACATCTACTTCTACA
CCATTAAGCCCATCCCTGCCAACCAGGAACTTCTTGTGTGGTATTGTCGGGACTTTTGCA
AAAGGCTTCACTACCCTTTTCNCGGAAAGCTTGACATGATGAAATCTTACACAAACCCCG
AGCAGTTCTAAAGCCACCCGACCCCTTGGAAAAAATGAACCTTTGCCCAAAGAATGTCCC
CAAAAAAGAGTCCAGGCGTGGAAAAAATCCTAAATTGGGCCCCCACCC
>OriGene 3' genomic read for NM_001198 unedited
GGGTTTGGGGCGCATGGGGATGGCACTTCCCGTTCCAGNGANAGCACTGGGGCAGGGTCA
CAGGATGCCACCCGGGNATCTGTTCAGAAAAGCTATGACCGCGGCCGCAATCTAGAGTCG
AGTTTTTTTTTTTTTTTTTTGGTAAAGCATGAACATTTATATTTAATTTACATTTTGACA
ATTTGCACATAAATAACATTGTAAACCAGTATGTAAACATAAGATAGTTTGTTGTTCTAG
CAAAGTAAAAAGCCAGTAACTTTGGTCAGTTTTAACTTTGAGTAGAGGAAAAAATAAAAC
CACTGTTGATTGTGGGCTCAACATTCACATGTAAGAGGCAGAAAAGAGAAGGATGGATGG
TGAGAGAAGCAAGAAGTCTTTATAAAAATTCTCACTATGGGAAAGCACAAAAGGAGAAAA
AGAACAAACGAGTAGTTGGGAATCAAAAGTATTTGGTCTAATGTCAACGTGATGCTTTGG
TTTACCTTCAGTTAAGGACTGTGTTTATCAAACACTGATACATAAAAAGCTGTCAGTCTT
GGAAGTTACAGAAGACCAGTAAGGCTCTTGGGTTTCCCTGGATTATTCGAAATTTGTTCT
CTTCTACAGGTGAACATTAGTTGATGTGAACAAAATGTCAAATATAATTATAAAATAGAT
TGGAGTAATGGATCAGTTTTTAGTAAGGAGGAAAACAGAAAATTGCAGCGGTAAAGTCAG
TTTTTTATTTCTGTGACTTGGGTGTGACCACTTAATTCTAAAGTGGCAAAAAAAAAATAG
ACCTGGCTTAAATGGGCCCTAATACCTAAAAAAGAGGTTTTCTCTTCCTCCCGGTGTGGT
TTGGGGGGCCCCCACCCTGGCACCAAG
Restriction Sites Please inquire     
ACCN NM_001198
Insert Size 5000 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001198.2, NP_001189.1
RefSeq Size 5165 bp
RefSeq ORF 2370 bp
Locus ID 639
Cytogenetics 6q21
Protein Families Transcription Factors
Gene Summary 'This gene encodes a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (1), also known as PRDM1alpha, encodes the longer isoform (1). The protein encoded by this transcript has also been named PRDI-BF1alpha.

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