PRDM1 (NM_001198) Human Untagged Clone

CAT#: SC302988

PRDM1 (untagged)-Human PR domain containing 1, with ZNF domain (PRDM1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRDM1
• Synonyms: BLIMP1; PRDI-BF1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001198 edited
  GACGCGGGGAGAATGTGGACTGGGTAGAGATGAACGAGACTTTTCTCAGATGTTGGATAT
  TTGCTTGGAAAAACGTGTGGGTACGACCTTGGCTGCCCCCAAGTGTAACTCCAGCACTGT
  GAGGTTTCAGGGATTGGCAGAGGGGACCAAGGGGACCATGAAAATGGACATGGAGGATGC
  GGATATGACTCTGTGGACAGAGGCTGAGTTTGAAGAGAAGTGTACATACATTGTGAACGA
  CCACCCCTGGGATTCTGGTGCTGATGGCGGTACTTCGGTTCAGGCGGAGGCATCCTTACC
  AAGGAATCTGCTTTTCAAGTATGCCACCAACAGTGAAGAGGTTATTGGAGTGATGAGTAA
  AGAATACATACCAAAGGGCACACGTTTTGGACCCCTAATAGGTGAAATCTACACCAATGA
  CACAGTTCCTAAGAACGCCAACAGGAAATATTTTTGGAGGATCTATTCCAGAGGGGAGCT
  TCACCACTTCATTGACGGCTTTAATGAAGAGAAAAGCAACTGGATGCGCTATGTGAATCC
  AGCACACTCTCCCCGGGAGCAAAACCTGGCTGCGTGTCAGAACGGGATGAACATCTACTT
  CTACACCATTAAGCCCATCCCTGCCAACCAGGAACTTCTTGTGTGGTATTGTCGGGACTT
  TGCAGAAAGGCTTCACTACCCTTATCCCGGAGAGCTGACAATGATGAATCTCACACAAAC
  ACAGAGCAGTCTAAAGCAACCGAGCACTGAGAAAAATGAACTCTGCCCAAAGAATGTCCC
  AAAGAGAGAGTACAGCGTGAAAGAAATCCTAAAATTGGACTCCAACCCCTCCAAAGGAAA
  GGACCTCTACCGTTCTAACATTTCACCCCTCACATCAGAAAAGGACCTCGATGACTTTAG
  AAGACGTGGGAGCCCCGAAATGCCCTTCTACCCTCGGGTCGTTTACCCCATCCGGGCCCC
  TCTGCCAGAAGACTTTTTGAAAGCTTCCCTGGCCTACGGGATCGAGAGACCCACGTACAT
  CACTCGCTCCCCCATTCCATCCTCCACCACTCCAAGCCCCTCTGCAAGAAGCAGCCCCGA
  CCAAAGCCTCAAGAGCTCCAGCCCTCACAGCAGCCCTGGGAATACGGTGTCCCCTGTGGG
  CCCCGGCTCTCAAGAGCACCGGGACTCCTACGCTTACTTGAACGCGTCCTACGGCACGGA
  AGGTTTGGGCTCCTACCCTGGCTACGCACCCCTGCCCCACCTCCCGCCAGCTTTCATCCC
  CTCGTACAACGCTCACTACCCCAAGTTCCTCTTGCCCCCCTACGGCATGAATTGTAATGG
  CCTGAGCGCTGTGAGCAGCATGAATGGCATCAACAACTTTGGCCTCTTCCCGAGGCTGTG
  CCCTGTCTACAGCAATCTCCTCGGTGGGGGCAGCCTGCCCCACCCCATGCTCAACCCCAC
  TTCTCTCCCGAGCTCGCTGCCCTCAGATGGAGCCCGGAGGTTGCTCCAGCCGGAGCATCC
  CAGGGAGGTGCTTGTCCCGGCGCCCCACAGTGCCTTCTCCTTTACCGGGGCCGCCGCCAG
  CATGAAGGACAAGGCCTGTAGCCCCACAAGCGGGTCTCCCACGGCGGGAACAGCCGCCAC
  GGCAGAACATGTGGTGCAGCCCAAAGCTACCTCAGCAGCGATGGCAGCCCCCAGCAGCGA
  CGAAGCCATGAATCTCATTAAAAACAAAAGAAACATGACCGGCTACAAGACCCTTCCCTA
  CCCGCTGAAGAAGCAGAACGGCAAGATCAAGTACGAATGCAACGTTTGCGCCAAGACTTT
  CGGCCAGCTCTCCAATCTGAAGGTCCACCTGAGAGTGCACAGTGGAGAACGGCCTTTCAA
  ATGTCAGACTTGCAACAAGGGCTTTACTCAGCTCGCCCACCTGCAGAAACACTACCTGGT
  ACACACGGGAGAAAAGCCACATGAATGCCAGGTCTGCCACAAGAGATTTAGCAGCACCAG
  CAATCTCAAGACCCACCTGCGACTCCATTCTGGAGAGAAACCATACCAATGCAAGGTGTG
  CCCTGCCAAGTTCACCCAGTTTGTGCACCTGAAACTGCACAAGCGTCTGCACACCCGGGA
  GCGGCCCCACAAGTGCTCCCAGTGCCACAAGAACTACATCCATCTCTGTAGCCTCAAGGT
  TCACCTGAAAGGGAACTGCGCTGCGGCCCCGGCGCCTGGGCTGCCCTTGGAAGATCTGAC
  CCGAATCAATGAAGAAATCGAGAAGTTTGACATCAGTGACAATGCTGACCGGCTCGAGGA
  CGTGGAGGATGACATCAGTGTGATCTCTGTAGTGGAGAAGGAAATTCTGGCCGTGGTCAG
  AAAAGAGAAAGAAGAAACTGGCCTGAAAGTGTCTTTGCAAAGAAACATGGGGAATGGACT
  CCTCTCCTCAGGGTGCAGCCTTTATGAGTCATCAGATCTACCCCTCATGAAGTTGCCTCC
  CAGCAACCCACTACCTCTGGTACCTGTAAAGGTCAAACAAGAAACAGTTGAACCAATGGA
  TCCTTAAGATTTTCAGAAAACACTTATTTTGTTTCTTAAGTTATGACTTGGTGAGTCAGG
  GTGCCTGTAGGAAGTGGCTTGTACATAATCCCAGCTCTGCAAAGCTCTCTCGACAGCAAA
  TGGTTTCCCCTCACCTCTGGAATTAAAGAAGGAACTCCAAAGTTACTGAAATCTCAGGGC
  ATGAACAAGGCAAAGGCCATATATATATATATATATATATCTGTATACATATTATATATA
  CTTATTTACACCTGTGTCTATATATTTGCCCCTGTGTATTTTGAATATTTGTGTGGACAT
  GTTTGCATAGCCTTCCCATTACTAAGACTATTACCTAGTCATAATTATTTTTTCAATGAT
  AATCCTTCATAATTTATTATACAATTTATCATTCAGAAAGCAATAATTAAAAAAGTTTAC
  AATGACTGGAAAGATTCCTTGTAATTTGAGTATAAATGTATTTTTGTCTTGTGGCCATTC
  TTTGTAGATAATTTCTGCACATCTGTATAAGTACCTAAGATTTAGTTAAACAAATATATG
  ACTTCAGTCAACCTCTCTCTCTAATAATGGTTTGAAAATGAGGTTTGGGTAATTGCCAAT
  GTTGGACAGTTGATGTGTTCATTCCTGGGATCCTATCATTTGAACAGCATTGTACATAAC
  TTGGGGGTATGTGTGCAGGATTACCCAAGAATAACTTAAGTAGAAGAAACAAGAAAGGGA
  ATCTTGTATATTTTTGTTGATAGTTCATGTTTTTCCCCCAGCCACAATTTTACCGGAAGG
  GTGACAGGAAGGCTTTACCAACCTGTCTCTCCCTCCAAAAGAGCAGAATCCTCCCACCGC
  CCTGCCCTCCCCACCGAGTCCTGTGGCCATTCAGAGCGGCCACATGACTTTTGCATCCAT
  TGTATTATCAGAAAATGTGAAGAAGAAAAAAATGCCATGTTTTAAAACCACTGCGAAAAT
  TTCCCCAAAGCATAGGTGGCTTTGTGTGTGTGCGATTTGGGGGCTTGAGTCTGGGTGGTG
  TTTTGTTGTTGGTTTTTGTTGCTTTTTTTTTTTTTTTTTTTTTAATGTCAAAATTGCACA
  AACATGGTGCTCTACCAGGAAGGATTCGAGGTAGATAGGCTCAGGCCACACTTTAAAAAC
  AAACACACAAACAACAAAAAACGGGTATTCTAGTCATCTTGGGGTAAAAGCGGGTAATGA
  ACATTCCTATCCCCAACACATCAATTGTATTTTTTCTGTAAAACTCAGATTTTCCTCAGT
  ATTTGTGTTTTTACATTTTATGGTTAATTTAATGGAAGATGAAAGGGCATTGCAAAGTTG
  TTCAACAACAGTTACCTCATTGAGTGTGTCCAGTAGTGCAGGAAATGATGTCTTATCTAA
  TGATTTGCTTCTCTAGAGGAGAAACCGAGTAAATGTGCTCCAGCAAGATAGACTTTGTGT
  TATTCTATCTTTTATTCTGCTAAGCCCAAAGATTACATGTTGGTGTTCAAAGTGTAGCAA
  AAAATGATGTATATTTATAAATCTATTTATACCACTATATCATATGTATATATATTTATA
  ACCACTTAAATTGTGAGCCAAGCCATGTAAAAGATCTACTTTTTCTAAGGGCAAAAAAAA
  AAAAAAAAAAAAAAGAACACTCCTTTCTGAGACTTTGCTTAATACTTGGTGACCTCACAA
  TCACGTCGGTATGATTGGGCACCCTTGCCTACTGTAAGAGACCCTAAAACCTTGGTGCAG
  TGGTGGGGACCACAAAACAACCAGGGAGGAAGAGATACATCATTTTTTAGTATTAAGGAC
  CATCTAAGACAGCTCTATTTTTTTTTTGCCACTTTATGATTATGTGGTCACACCCAAGTC
  ACAGAAATAAAAAACTGACTTTACCGCTGCAATTTTTCTGTTTTCCTCCTTACTAAATAC
  TGATACATTACTCCAATCTATTTTATAATTATATTTGACATTTTGTTCACATCAACTAAT
  GTTCACCTGTAGAAGAGAACAAATTTCGAATAATCCAGGGAAACCCAAGAGCCTTACTGG
  TCTTCTGTAACTTCCAAGACTGACAGCTTTTTATGTATCAGTGTTTGATAAACACAGTCC
  TTAACTGAAGGTAAACCAAAGCATCACGTTGACATTAGACCAAATACTTTTGATTCCCAA
  CTACTCGTTTGTTCTTTTTCTCCTTTTGTGCTTTCCCATAGTGAGAATTTTTATAAAGAC
  TTCTTGCTTCTCTCACCATCCATCCTTCTCTTTTCTGCCTCTTACATGTGAATGTTGAGC
  CCACAATCAACAGTGGTTTTATTTTTTCCTCTACTCAAAGTTAAAACTGACCAAAGTTAC
  TGGCTTTTTACTTTGCTAGAACAACAAACTATCTTATGTTTACATACTGGTTTACAATGT
  TATTTATGTGCAAATTGTCAAAATGTAAATTAAATATAAATGTTCATGCTTTACCAAAAA
  AAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001198 unedited
  TTTGTACGGTCTCCATTTGTATACGACTCATATAGGCGGCCGCAGATTCGCCCTTGACGC
  GGGGAGAATGTGGACTGGGTAGAGATGAACGAGACTTTTCTCAGATGTTGGATATTTGCT
  TGGAAAAACGTGTGGGTACGACCTTGGCTGCCCCCAAGTGTAACTCCAGCACTGTGAGGT
  TTCAGGGATTGGCAGAGGGGACCAAGGGGACCATGAAAATGGACATGGAGGATGCGGATA
  TGACTCTGTGGACAGAGGCTGAGTTTGAAGAGAAGTGTACATACATTGTGAACGACCACC
  CCTGGGATTCTGGTGCTGATGGCGGTACTTCGGTTCAGGCGGAGGCATCCTTACCAAGGA
  ATCTGCTTTTCAAGTATGCCACCAACAGTGAAGAGGTTATTGGAGTGATGAGTAAAGAAT
  ACATACCAAAGGGCACACGTTTTGGACCCCTAATAGGTGAAATCTACACCAATGACACAG
  TTCCTAAGAACGCCAACAGGAAATATTTTTGGAGGATCTATTCCAGAGGGGAGCTTCACC
  ACTTCATTGACGGCTNTAATGAAGAGAAAAAGCACTGGATGCGCTATGTGAATCCAGCAC
  ACTCTCCCCGGGAGCAAAACCTGNCTGCGTGTCAGAACGGGATGAACATCTACTTCTACA
  CCATTAAGCCCATCCCTGCCAACCAGGAACTTCTTGTGTGGTATTGTCGGGACTTTTGCA
  AAAGGCTTCACTACCCTTTTCNCGGAAAGCTTGACATGATGAAATCTTACACAAACCCCG
  AGCAGTTCTAAAGCCACCCGACCCCTTGGAAAAAATGAACCTTTGCCCAAAGAATGTCCC
  CAAAAAAGAGTCCAGGCGTGGAAAAAATCCTAAATTGGGCCCCCACCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_001198 unedited
  GGGTTTGGGGCGCATGGGGATGGCACTTCCCGTTCCAGNGANAGCACTGGGGCAGGGTCA
  CAGGATGCCACCCGGGNATCTGTTCAGAAAAGCTATGACCGCGGCCGCAATCTAGAGTCG
  AGTTTTTTTTTTTTTTTTTTGGTAAAGCATGAACATTTATATTTAATTTACATTTTGACA
  ATTTGCACATAAATAACATTGTAAACCAGTATGTAAACATAAGATAGTTTGTTGTTCTAG
  CAAAGTAAAAAGCCAGTAACTTTGGTCAGTTTTAACTTTGAGTAGAGGAAAAAATAAAAC
  CACTGTTGATTGTGGGCTCAACATTCACATGTAAGAGGCAGAAAAGAGAAGGATGGATGG
  TGAGAGAAGCAAGAAGTCTTTATAAAAATTCTCACTATGGGAAAGCACAAAAGGAGAAAA
  AGAACAAACGAGTAGTTGGGAATCAAAAGTATTTGGTCTAATGTCAACGTGATGCTTTGG
  TTTACCTTCAGTTAAGGACTGTGTTTATCAAACACTGATACATAAAAAGCTGTCAGTCTT
  GGAAGTTACAGAAGACCAGTAAGGCTCTTGGGTTTCCCTGGATTATTCGAAATTTGTTCT
  CTTCTACAGGTGAACATTAGTTGATGTGAACAAAATGTCAAATATAATTATAAAATAGAT
  TGGAGTAATGGATCAGTTTTTAGTAAGGAGGAAAACAGAAAATTGCAGCGGTAAAGTCAG
  TTTTTTATTTCTGTGACTTGGGTGTGACCACTTAATTCTAAAGTGGCAAAAAAAAAATAG
  ACCTGGCTTAAATGGGCCCTAATACCTAAAAAAGAGGTTTTCTCTTCCTCCCGGTGTGGT
  TTGGGGGGCCCCCACCCTGGCACCAAG
  
• Restriction Sites: Please inquire
• ACCN: NM_001198
• Insert Size: 5000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001198.2, NP_001189.1
• RefSeq Size: 5165 bp
• RefSeq ORF: 2370 bp
• Locus ID: 639
• Cytogenetics: 6q21
• Protein Families: Transcription Factors
• Gene Summary: 'This gene encodes a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1), also known as PRDM1alpha, encodes the longer isoform (1). The protein encoded by this transcript has also been named PRDI-BF1alpha.