PRDM1 (NM_001198) Human Untagged Clone

CAT#: SC328015

PRDM1 (untagged)-Human PR domain containing 1 with ZNF domain (PRDM1) transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRDM1
• Synonyms: BLIMP1; PRDI-BF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001198, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTTGGATATTTGCTTGGAAAAACGTGTGGGTACGACCTTGGCTGCCCCCAAGTGTAACTCCAGCACTG
  TGAGGTTTCAGGGATTGGCAGAGGGGACCAAGGGGACCATGAAAATGGACATGGAGGATGCGGATATGAC
  TCTGTGGACAGAGGCTGAGTTTGAAGAGAAGTGTACATACATTGTGAACGACCACCCCTGGGATTCTGGT
  GCTGATGGCGGTACTTCGGTTCAGGCGGAGGCATCCTTACCAAGGAATCTGCTTTTCAAGTATGCCACCA
  ACAGTGAAGAGGTTATTGGAGTGATGAGTAAAGAATACATACCAAAGGGCACACGTTTTGGACCCCTAAT
  AGGTGAAATCTACACCAATGACACAGTTCCTAAGAACGCCAACAGGAAATATTTTTGGAGGATCTATTCC
  AGAGGGGAGCTTCACCACTTCATTGACGGCTTTAATGAAGAGAAAAGCAACTGGATGCGCTATGTGAATC
  CAGCACACTCTCCCCGGGAGCAAAACCTGGCTGCGTGTCAGAACGGGATGAACATCTACTTCTACACCAT
  TAAGCCCATCCCTGCCAACCAGGAACTTCTTGTGTGGTATTGTCGGGACTTTGCAGAAAGGCTTCACTAC
  CCTTATCCCGGAGAGCTGACAATGATGAATCTCACACAAACACAGAGCAGTCTAAAGCAACCGAGCACTG
  AGAAAAATGAACTCTGCCCAAAGAATGTCCCAAAGAGAGAGTACAGCGTGAAAGAAATCCTAAAATTGGA
  CTCCAACCCCTCCAAAGGAAAGGACCTCTACCGTTCTAACATTTCACCCCTCACATCAGAAAAGGACCTC
  GATGACTTTAGAAGACGTGGGAGCCCCGAAATGCCCTTCTACCCTCGGGTCGTTTACCCCATCCGGGCCC
  CTCTGCCAGAAGACTTTTTGAAAGCTTCCCTGGCCTACGGGATCGAGAGACCCACGTACATCACTCGCTC
  CCCCATTCCATCCTCCACCACTCCAAGCCCCTCTGCAAGAAGCAGCCCCGACCAAAGCCTCAAGAGCTCC
  AGCCCTCACAGCAGCCCTGGGAATACGGTGTCCCCTGTGGGCCCCGGCTCTCAAGAGCACCGGGACTCCT
  ACGCTTACTTGAACGCGTCCTACGGCACGGAAGGTTTGGGCTCCTACCCTGGCTACGCACCCCTGCCCCA
  CCTCCCGCCAGCTTTCATCCCCTCGTACAACGCTCACTACCCCAAGTTCCTCTTGCCCCCCTACGGCATG
  AATTGTAATGGCCTGAGCGCTGTGAGCAGCATGAATGGCATCAACAACTTTGGCCTCTTCCCGAGGCTGT
  GCCCTGTCTACAGCAATCTCCTCGGTGGGGGCAGCCTGCCCCACCCCATGCTCAACCCCACTTCTCTCCC
  GAGCTCGCTGCCCTCAGATGGAGCCCGGAGGTTGCTCCAGCCGGAGCATCCCAGGGAGGTGCTTGTCCCG
  GCGCCCCACAGTGCCTTCTCCTTTACCGGGGCCGCCGCCAGCATGAAGGACAAGGCCTGTAGCCCCACAA
  GCGGGTCTCCCACGGCGGGAACAGCCGCCACGGCAGAACATGTGGTGCAGCCCAAAGCTACCTCAGCAGC
  GATGGCAGCCCCCAGCAGCGACGAAGCCATGAATCTCATTAAAAACAAAAGAAACATGACCGGCTACAAG
  ACCCTTCCCTACCCGCTGAAGAAGCAGAACGGCAAGATCAAGTACGAATGCAACGTTTGCGCCAAGACTT
  TCGGCCAGCTCTCCAATCTGAAGGTCCACCTGAGAGTGCACAGTGGAGAACGGCCTTTCAAATGTCAGAC
  TTGCAACAAGGGCTTTACTCAGCTCGCCCACCTGCAGAAACACTACCTGGTACACACGGGAGAAAAGCCA
  CATGAATGCCAGGTCTGCCACAAGAGATTTAGCAGCACCAGCAATCTCAAGACCCACCTGCGACTCCATT
  CTGGAGAGAAACCATACCAATGCAAGGTGTGCCCTGCCAAGTTCACCCAGTTTGTGCACCTGAAACTGCA
  CAAGCGTCTGCACACCCGGGAGCGGCCCCACAAGTGCTCCCAGTGCCACAAGAACTACATCCATCTCTGT
  AGCCTCAAGGTTCACCTGAAAGGGAACTGCGCTGCGGCCCCGGCGCCTGGGCTGCCCTTGGAAGATCTGA
  CCCGAATCAATGAAGAAATCGAGAAGTTTGACATCAGTGACAATGCTGACCGGCTCGAGGACGTGGAGGA
  TGACATCAGTGTGATCTCTGTAGTGGAGAAGGAAATTCTGGCCGTGGTCAGAAAAGAGAAAGAAGAAACT
  GGCCTGAAAGTGTCTTTGCAAAGAAACATGGGGAATGGACTCCTCTCCTCAGGGTGCAGCCTTTATGAGT
  CATCAGATCTACCCCTCATGAAGTTGCCTCCCAGCAACCCACTACCTCTGGTACCTGTAAAGGTCAAACA
  AGAAACAGTTGAACCAATGGATCCTTAA
  
  
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001198
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001198.3, NP_001189.2
• RefSeq Size: 5165 bp
• RefSeq ORF: 2478 bp
• Locus ID: 639
• Cytogenetics: 6q21
• Protein Families: Transcription Factors
• Gene Summary: 'This gene encodes a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1), also known as PRDM1alpha, encodes the longer isoform (1). The protein encoded by this transcript has also been named PRDI-BF1alpha.