LIF (NM_002309) Human Untagged Clone

CAT#: SC303176

LIF (untagged)-Human leukemia inhibitory factor (cholinergic differentiation factor) (LIF)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LIF
• Synonyms: CDF; DIA; HILDA; MLPLI
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002309 edited
  ATGAAGGTCTTGGCGGCAGGAGTTGTGCCCCTGCTGTTGGTTCTGCACTGGAAACATGGG
  GCGGGGAGCCCCCTCCCCATCACCCCTGTCAACGCCACCTGTGCCATACGCCACCCATGT
  CACAACAACCTCATGAACCAGATCAGGAGCCAACTGGCACAGCTCAATGGCAGTGCCAAT
  GCCCTCTTTATTCTCTATTACACAGCCCAGGGGGAGCCGTTCCCCAACAACCTGGACAAG
  CTATGTGGCCCCAACGTGACGGACTTCCCGCCCTTCCACGCCAACGGCACGGAGAAGGCC
  AAGCTGGTGGAGCTGTACCGCATAGTCGTGTACCTTGGCACCTCCCTGGGCAACATCACC
  CGGGACCAGAAGATCCTCAACCCCAGTGCCCTCAGCCTCCACAGCAAGCTCAACGCCACC
  GCCGACATCCTGCGAGGCCTCCTTAGCAACGTGCTGTGCCGCCTGTGCAGCAAGTACCAC
  GTGGGCCATGTGGACGTGACCTACGGCCCTGACACCTCGGGTAAGGATGTCTTCCAGAAG
  AAGAAGCTGGGCTGTCAACTCCTGGGGAAGTATAAGCAGATCATCGCCGTGTTGGCCCAG
  GCCTTCTAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002309 unedited
  NGAGTACAAAATTTGTATACGACTCACTATAGGCGGCCGCGNATTCATGAAGTCTTGGCG
  GCAGGAGTTGTGCCCCTGCTGTTGGTTCTGCACTGGAAACATGGGGCGGGGAGCCCCCTC
  CCCATCACCCCTGTCAACGCCACCTGTGCCATACGCCACCCATGTCACAACAACCTCATG
  AACCAGATCAGGAGCCAACTGGCACAGCTCAATGGCAGTGCCAATGCCCTCTTTATTCTC
  TATTACACAGCCCAGGGGGAGCCGTTCCCCAACAACCTGGACAAGCTATGTGGCCCCAAC
  GTGACGGACTTCCCGCCCTTCCACGCCAACGGCACGGAGAAGGCCAAGCTGGTGGAGCTG
  TACCGCATAGTCGTGTACCTTGGCACCTCCCTGGGCAACATCACCCGGGACCAGAAGATC
  CTCAACCCCAGTGCCCTCAGCCTCCACAGCAAGCTCAACGCCACCGCCGACATCCTGCGA
  GGCCTCCTTAGCAACGTGCTGTGCCGCCTGTGCAGCAAGTACCACGTGGGCCATGTGGAC
  GTGACCTACGGCCCTGACACCTCGGGTAAGGATGTCTTCCAGAAGAAGAAGCTGGGCTGT
  CAACTCCTGNGGAAGTATAAGCAGATCATCGCCGTGTTGGCCCAGGCCTTCTAGGTCGAC
  TCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTG
  ACCCCTCCCCAGTGCCTCTCCTGGCCCTGGGAGTTGCCACTCCAGTGCCCACCAGCCTTG
  TCCTAATAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATATATTATGGGG
  TGGGGGGGGTGGGGTATGGAACAGGGGGCAAAGTGGGGAAGAAACCTGTAGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002309 unedited
  GNANGAGCACTGGGGNAGGGTCACAGGGATGCCACCCGGGATCTGTTCAGGAAACAGCTA
  TGACCGCGGCCGCAATCTAGAGTCGACCTAGAAGGCCTGGGCCAACACGGCGATGATCTG
  CTTATACTTCCCCAGGAGTTGACAGCCCAGCTTCTTCTTCTGGAAGACATCCTTACCCGA
  GGTGTCAGGGCCGTAGGTCACGTCCACATGGCCCACGTGGTACTTGCTGCACAGGCGGCA
  CAGCACGTTGCTAAGGAGGCCTCGCAGGATGTCGGCGGTGGCGTTGAGCTTGCTGTGGAG
  GCTGAGGGCACTGGGGTTGAGGATCTTCTGGTCCCGGGTGATGTTGCCCAGGGAGGTGCC
  AAGGTACACGACTATGCGGTACAGCTCCACCAGCTTGGCCTTCTCCGTGCCGTTGGCGTG
  GAAGGGCGGGAAGTCCGTCACGTTGGGGCCACATAGCTTGTCCAGGTTGTTGGGGAACGG
  CTCCCCCTGGGCTGTGTAATAGAGAATAAAGAGGGCATTGGCACTGCCATTGAGCTGTGC
  CAGTTGGCTCCTGATCTGGTTCATGAGGTTGTTGTGACATGGGTGGCGTATGGCACAGGT
  GGCGTTGACAGGGGTATGGGGAAAAGGGGCCTTCCCGCCCATGTTTCCAGTGCAGAACCA
  ACAGCAGGGGCACAACTCCTGCCGCCAAGACCTTCATGAATTCGCGGCCGCCCTATAGTG
  AGTCGTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTATATAGACCTCCCAC
  CGTACACGCCTACCGCCCATTTGCGTCAACGGGGGCGNGGTTATTACGACATTTTGGAAA
  GTCCCGTTGATTT
  
• Restriction Sites: Please inquire
• ACCN: NM_002309
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002309.2, NP_002300.1
• RefSeq Size: 3868 bp
• RefSeq ORF: 609 bp
• Locus ID: 3976
• Cytogenetics: 22q12.2
• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway
• Gene Summary: 'The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.