SIM2 (NM_005069) Human Untagged Clone

CAT#: SC303574

SIM2 (untagged)-Human single-minded homolog 2 (Drosophila) (SIM2), transcript variant SIM2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SIM2
• Synonyms: bHLHe15; HMC13F06; HMC29C01; SIM
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC303574 sequence for NM_005069 edited (data generated by NextGen Sequencing)
  
  
  ATGAAGGAGAAGTCCAAGAATGCGGCCAAGACCAGGAGGGAGAAGGAAAATGGCGAGTTTTACGAGCTTG
  CCAAGCTGCTCCCGCTGCCGTCGGCCATCACTTCGCAGCTGGACAAAGCGTCCATCATCCGCCTCACCAC
  GAGCTACCTGAAGATGCGCGCCGTCTTCCCCGAAGGTTTAGGAGACGCGTGGGGACAGCCGAGCCGCGCC
  GGGCCCCTGGACGGCGTCGCCAAGGAGCTGGGATCGCACTTGCTGCAGACTTTGGATGGATTTGTTTTTG
  TGGTAGCATCTGATGGCAAAATCATGTATATATCCGAGACCGCTTCTGTCCATTTAGGCTTATCCCAGGT
  GGAGCTCACGGGCAACAGTATTTATGAATACATCCATCCTTCTGACCACGATGAGATGACCGCTGTCCTC
  ACGGCCCACCAGCCGCTGCACCACCACCTGCTCCAAGAGTATGAGATAGAGAGGTCGTTCTTTCTTCGAA
  TGAAATGTGTCTTGGCGAAAAGGAACGCGGGCCTGACCTGCAGCGGATACAAGGTCATCCACTGCAGTGG
  CTACTTGAAGATCAGGCAGTATATGCTGGACATGTCCCTGTACGACTCCTGCTACCAGATTGTGGGGCTG
  GTGGCCGTGGGCCAGTCGCTGCCACCCAGTGCCATCACCGAGATCAAGCTGTACAGTAACATGTTCATGT
  TCAGGGCCAGCCTTGACCTGAAGCTGATATTCCTGGATTCCAGGGTGACCGAGGTGACGGGGTACGAGCC
  GCAGGACCTGATCGAGAAGACCCTATACCATCACGTGCACGGCTGCGACGTGTTCCACCTCCGCTACGCA
  CACCACCTCCTGTTGGTGAAGGGCCAGGTCACCACCAAGTACTACCGGCTGCTGTCCAAGCGGGGCGGCT
  GGGTGTGGGTGCAGAGCTACGCCACCGTGGTGCACAACAGCCGCTCGTCCCGGCCCCACTGCATCGTGAG
  TGTCAATTATGTACTCACGGAGATTGAATACAAGGAACTTCAGCTGTCCCTGGAGCAGGTGTCCACTGCC
  AAGTCCCAGGACTCCTGGAGGACCGCCTTGTCTACCTCACAAGAAACTAGGAAATTAGTGAAACCCAAAA
  ATACCAAGATGAAGACAAAGCTGAGAACAAACCCTTACCCCCCACAGCAATACAGCTCGTTCCAAATGGA
  CAAACTGGAATGCGGCCAGCTCGGAAACTGGAGAGCCAGTCCCCCTGCAAGCGCTGCTGCTCCTCCAGAA
  CTGCAGCCCCACTCAGAAAGCAGTGACCTTCTGTACACGCCATCCTACAGCCTGCCCTTCTCCTACCATT
  ACGGACACTTCCCTCTGGACTCTCACGTCTTCAGCAGCAAAAAGCCAATGTTGCCGGCCAAGTTCGGGCA
  GCCCCAAGGATCCCCTTGTGAGGTGGCACGCTTTTTCCTGAGCACAATGCCAGCCAGCGGTGAATGCCAG
  TGGCATTATGCCAACCCCCTAGTGCCTAGCAGCTCGTCTCCAGCTAAAAATCCTCCAGAGCCACCGGCGA
  ACACTGCTAGGCACAGCCTGGTGCCAAGCTACGAAGCGCCCGCCGCCGCCGTGCGCAGGTTCGGCGAGGA
  CACCGCGCCCCCGAGCTTCCCGAGCTGCGGCCACTACCGCGAGGAGCCCGCGCTGGGCCCGGCCAAAGCC
  GCCCGCCAGGCCGCCCGGGACGGGGCGCGGCTGGCGCTGGCCCGCGCGGCACCCGAGTGCTGCGCGCCCC
  CGACCCCCGAGGCCCCGGGCGCGCCGGCGCAGCTGCCCTTCGTGCTGCTCAACTACCACCGCGTGCTGGC
  CCGGCGCGGACCGCTGGGGGGCGCCGCACCCGCCGCCTCCGGCCTGGCCTGCGCTCCCGGCGGCCCCGAG
  GCGGCGACCGGCGCGCTGCGGCTCCGGCACCCGAGCCCCGCCGCCACCTCCCCGCCCGGCGCGCCCCTGC
  CGCACTACCTGGGCGCCTCGGTCATCATCACCAACGGGAGGTGA
  
  Clone variation with respect to NM_005069.5
  1447:a=>c
• Restriction Sites: Please inquire
• ACCN: NM_005069
• Insert Size: 3700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005069.2, NP_005060.1
• RefSeq Size: 4455 bp
• RefSeq ORF: 2004 bp
• Locus ID: 6493
• Cytogenetics: 21q22.13
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'This gene represents a homolog of the Drosophila single-minded (sim) gene, which encodes a transcription factor that is a master regulator of neurogenesis. The encoded protein is ubiquitinated by RING-IBR-RING-type E3 ubiquitin ligases, including the parkin RBR E3 ubiquitin protein ligase. This gene maps within the so-called Down syndrome chromosomal region, and is thus thought to contribute to some specific Down syndrome phenotypes. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2014]'
  Transcript Variant: This variant (SIM2) represents the longer transcript and encodes the longer isoform (long).