SIM2 (NM_009586) Human Untagged Clone

CAT#: SC303922

SIM2 (untagged)-Human single-minded homolog 2 (Drosophila) (SIM2), transcript variant SIM2s

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SIM2
• Synonyms: bHLHe15; HMC13F06; HMC29C01; SIM
• Vector: PCMV6-Neo
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_009586 edited
  GCGATGAAGGAGAAGTCCAAGAATGCGGCCAAGACCAGGAGGGAGAAGGAAAATGGCGAG
  TTTTACGAGCTTGCCAAGCTGCTCCCGCTGCCGTCGGCCATCACTTCGCAGCTGGACAAA
  GCGTCCATCATCCGCCTCACCACGAGCTACCTGAAGATGCGCGCCGTCTTCCCCGAAGGT
  TTAGGAGACGCGTGGGGACAGCCGAGCCGCGCCGGGCCCCTGGACGGCGTCGCCAAGGAG
  CTGGGATCGCACTTGCTGCAGACTTTGGATGGATTTGTTTTTGTGGTAGCATCTGATGGC
  AAAATCATGTATATATCCGAGACCGCTTCTGTCCATTTAGGCTTATCCCAGGTGGAGCTC
  ACGGGCAACAGTATTTATGAATACATCCATCCTTCTGACCACGATGAGATGACCGCTGTC
  CTCACGGCCCACCAGCCGCTGCACCACCACCTGCTCCAAGAGTATGAGATAGAGAGGTCG
  TTCTTTCTTCGAATGAAATGTGTCTTGGCGAAAAGGAACGCGGGCCTGACCTGCAGCGGA
  TACAAGGTCATCCACTGCAGTGGCTACTTGAAGATCAGGCAGTATATGCTGGACATGTCC
  CTGTACGACTCCTGCTACCAGATTGTGGGGCTGGTGGCCGTGGGCCAGTCGCTGCCACCC
  AGTGCCATCACCGAGATCAAGCTGTACAGTAACATGTTCATGTTCAGGGCCAGCCTTGAC
  CTGAAGCTGATATTCCTGGATTCCAGGGTGACCGAGGTGACGGGTTACGAGCCGCAGGAC
  CTGATCGAGAAGACCCTATACCATCACGTGCACGGCTGCGACGTGTTCCACCTCCGCTAC
  GCACACCACCTCCTGTTGGTGAAGGGCCAGGTCACCACCAAGTACTACCGGCTGCTGTCC
  AAGCGGGGCGGCTGGGTGTGGGTGCAGAGCTACGCCACCGTGGTGCACAACAGCCGCTCG
  TCCCGGCCCCACTGCATCGTGAGTGTCAATTATGTACTCACGGAGATTGAATACAAGGAA
  CTTCAGCTGTCCCTGGAGCAGGTGTCCACTGCCAAGTCCCAGGACTCCTGGAGGACCGCC
  TTGTCTACCTCACAAGAAACTAGGAAATTAGTGAAACCCAAAAATACCAAGATGAAGACA
  AAGCTGAGAACAAACCCTTACCCCCCACAGCAATACAGCTCGTTCCAAATGGACAAACTG
  GAATGCGGCCAGCTCGGAAACTGGAGAGCCAGTCCCCCTGCAAGCGCTGCTGCTCCTCCA
  GAACTGCAGCCCCACTCAGAAAGCAGTGACCTTCTGTACACGCCATCCTACAGCCTGCCC
  TTCTCCTACCATTACGGACACTTCCCTCTGGACTCTCACGTCTTCAGCAGCAAAAAGCCA
  ATGTTGCCGGCCAAGTTCGGGCAGCCCCAAGGATCCCCTTGTGAGGTGGCACGCTTTTTC
  CTGAGCACACTGCCAGCCAGCGGTGAATGCCAGTGGCATTATGCCAACCCCCTAGTGCCT
  AGCAGCTCGTCTCCAGCTAAAAATCCTCCAGAGCCACCGGCGAACACTGCTAGGCACAGC
  CTGGTGCCAAGCTACGAAGGTGGGTCAGGTCTGCTCGTGGGGAAGGTGGGAGGACTGCGC
  ACGGCCGGGAGCCGAAGCAGCCATGGCGGTGGGTGGCAGATGGAGACAGAACCCTCACGC
  TTTGGGCAAACTTGCCCTCTTTCTGCTTCTAAGTAGGGCTTGCTGTGCTTTCTTGCTCT
  
• Restriction Sites: Please inquire
• ACCN: NM_009586
• Insert Size: 1800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to contain one SNP compared with NM_009586.1.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009586.1, NP_033664.1
• RefSeq Size: 2823 bp
• RefSeq ORF: 1713 bp
• Locus ID: 6493
• Cytogenetics: 21q22.13
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'This gene represents a homolog of the Drosophila single-minded (sim) gene, which encodes a transcription factor that is a master regulator of neurogenesis. The encoded protein is ubiquitinated by RING-IBR-RING-type E3 ubiquitin ligases, including the parkin RBR E3 ubiquitin protein ligase. This gene maps within the so-called Down syndrome chromosomal region, and is thus thought to contribute to some specific Down syndrome phenotypes. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2014]'
  Transcript Variant: This variant (SIM2s) contains alternate 3' exon structure, and it thus differs in the 3' coding region and 3' UTR, compared to variant SIM2. The encoded isoform (short) has a distinct C-terminus and is shorter than isoform long.