ANK1 (NM_020477) Human Untagged Clone

CAT#: SC304776

ANK1 (untagged)-Human ankyrin 1, erythrocytic (ANK1), transcript variant 2


  "NM_020477" in other vectors (2)

Reconstitution Protocol

USD 3,480.00

8 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol ANK1
Synonyms ANK; SPH1; SPH2
Vector pCMV6 series
Sequence Data
>NCBI ORF sequence for NM_020477, the custom clone sequence may differ by one or more nucleotides
ATGCCCTATTCTGTGGGCTTCCGCGAAGCCGATGCTGCTACCAGCTTTCTGAGAGCAGCA
AGATCAGGTAACTTGGACAAAGCTTTGGATCACCTGCGGAATGGGGTAGATATTAACACC
TGTAACCAGAATGGGTTGAATGGCTTGCATCTGGCTTCTAAGGAAGGCCATGTGAAAATG
GTGGTTGAACTTCTGCACAAAGAAATCATTCTAGAAACGACAACCAAGAAGGGGAACACG
GCCCTGCACATCGCTGCTCTAGCCGGGCAGGATGAGGTGGTCCGGGAGCTTGTCAACTAT
GGAGCCAACGTCAACGCCCAGTCACAGAAAGGTTTTACACCCCTGTACATGGCAGCACAA
GAGAACCACTTGGAAGTGGTTAAGTTTTTACTGGAAAATGGAGCTAACCAGAATGTAGCC
ACAGAAGACGGCTTCACGCCTCTGGCGGTAGCCCTGCAGCAGGGCCATGAGAACGTCGTC
GCGCACCTCATCAACTACGGCACCAAGGGGAAGGTGCGCCTCCCGGCCCTGCACATCGCG
GCCCGCAACGACGACACGCGCACGGCTGCGGTGCTGCTGCAGAACGACCCCAACCCGGAC
GTGCTTTCCAAGACGGGATTCACGCCCCTGCACATTGCGGCTCACTACGAGAACCTCAAC
GTGGCCCAGTTGCTCCTCAACAGAGGAGCCAGCGTCAATTTCACACCACAGAACGGCATC
ACGCCACTGCACATCGCCTCCCGCAGGGGCAACGTGATCATGGTGCGGCTGCTGCTGGAT
CGGGGAGCCCAGATAGAAACCAAGACCAAGGACGAATTGACACCTCTCCACTGTGCAGCT
CGAAATGGGCACGTGCGAATCTCAGAGATCCTGCTGGACCACGGGGCACCAATCCAAGCC
AAAACCAAGAACGGCCTGTCCCCAATTCACATGGCGGCTCAGGGAGACCACCTCGACTGT
GTCCGGCTCCTGTTGCAATACGACGCAGAGATAGACGACATCACCCTGGACCACCTGACC
CCACTCCACGTGGCTGCCCACTGTGGACACCACAGGGTGGCTAAGGTCCTTCTGGATAAA
GGGGCCAAACCCAACTCCAGAGCCCTGAATGGCTTTACCCCCTTACACATCGCCTGCAAA
AAGAACCACGTCCGTGTCATGGAGCTGCTGCTGAAGACGGGAGCCTCGATCGACGCGGTC
ACCGAGTCTGGCCTGACACCTCTCCACGTGGCCTCCTTCATGGGGCACCTTCCCATCGTG
AAGAACCTCCTGCAGCGGGGGGCGTCGCCCAACGTCTCCAACGTGAAAGTGGAGACCCCG
CTACACATGGCAGCCAGAGCCGGGCACACGGAAGTGGCCAAATATTTACTCCAGAACAAA
GCCAAAGTCAATGCCAAGGCCAAGGATGACCAGACCCCACTTCACTGTGCAGCTCGCATC
GGCCACACAAACATGGTGAAGCTCCTGCTGGAAAATAACGCCAACCCCAACCTGGCCACC
ACCGCCGGGCACACCCCCCTGCACATTGCAGCCCGTGAGGGCCATGTGGAAACAGTCCTG
GCCCTTCTGGAAAAGGAAGCATCCCAGGCCTGCATGACCAAGAAAGGATTTACCCCTCTG
CACGTGGCGGCCAAGTACGGGAAGGTGCGGGTGGCAGAGCTGCTGCTGGAGCGGGACGCA
CACCCGAATGCTGCCGGAAAAAATGGCCTGACCCCCCTGCACGTGGCCGTCCATCACAAC
AACCTGGACATCGTCAAGCTGCTGCTTCCCCGGGGCGGCTCCCCGCACAGCCCTGCCTGG
AATGGCTACACCCCTTTGCACATCGCTGCCAAGCAGAACCAGGTGGAGGTGGCCCGTAGT
CTGCTGCAGTATGGGGGCTCAGCAAACGCCGAGTCGGTGCAAGGTGTGACGCCCCTTCAC
CTGGCCGCCCAGGAGGGCCACGCAGAGATGGTGGCTCTGCTGCTCTCGAAACAAGCCAAT
GGCAACCTGGGGAACAAGAGCGGACTCACTCCCCTCCATCTGGTAGCACAAGAAGGCCAC
GTTCCAGTGGCAGATGTGCTGATCAAACACGGCGTCATGGTGGACGCCACCACCCGGATG
GGCTACACTCCCCTCCATGTGGCCAGTCACTATGGAAACATCAAGCTGGTGAAGTTTCTG
CTGCAGCACCAGGCAGATGTCAATGCCAAGACCAAGCTAGGATACAGCCCCCTGCACCAG
GCAGCCCAGCAGGGACACACAGACATCGTGACTCTGCTTCTGAAAAACGGTGCTTCCCCA
AACGAGGTCAGCTCGGATGGAACCACACCTCTGGCCATAGCCAAGCGCTTGGGCTACATT
TCTGTCACCGACGTGCTCAAGGTCGTCACGGATGAAACCAGTTTCGTGTTAGTCAGTGAT
AAGCATCGAATGAGTTTCCCTGAGACAGTTGATGAGATCCTGGATGTCTCGGAAGATGAA
GGGGAAGAACTCATCAGCTTCAAGGCTGAGAGGCGGGATTCCAGGGATGTTGATGAAGAG
AAGGAGCTGCTGGATTTTGTGCCGAAGCTAGACCAAGTGGTGGAATCTCCAGCCATCCCC
AGGATTCCCTGTGCCATGCCTGAGACAGTGGTGATCAGGTCAGAAGAGCAGGAGCAGGCA
TCTAAAGAGTATGATGAGGACTCCCTCATCCCCAGCAGCCCGGCCACCGAGACCTCAGAC
AACATCAGCCCGGTGGCCAGCCCGGTGCATACAGGGTTTCTGGTGAGCTTCATGGTTGAC
GCCCGGGGTGGTTCCATGAGAGGAAGTCGCCACAACGGCCTGCGAGTGGTGATCCCGCCA
CGGACGTGCGCAGCGCCCACCCGCATCACCTGCCGCCTGGTCAAGCCCCAGAAGCTCAGC
ACGCCGCCCCCACTGGCCGAGGAGGAGGGCCTGGCCAGCAGGATCATAGCACTGGGGCCC
ACGGGGGCACAGTTCCTGAGCCCTGTAATCGTGGAGATCCCGCACTTTGCCTCCCATGGC
CGTGGAGACCGCGAGCTCGTGGTTCTGAGGAGCGAAAACGGCTCCGTGTGGAAGGAGCAC
AGGAGCCGCTATGGAGAGAGCTACCTGGATCAGATCCTCAACGGGATGGACGAAGAGCTG
GGGAGCCTGGAGGAGCTAGAGAAGAAGAGGGTGTGCCGAATCATCACCACCGACTTCCCG
CTGTACTTCGTGATCATGTCACGGCTCTGCCAGGACTACGACACCATCGGTCCCGAAGGG
GGCTCCCTGAAGAGCAAGCTGGTGCCCCTGGTACAGGCAACGTTCCCGGAGAATGCCGTC
ACCAAGAGAGTGAAGCTGGCTCTGCAGGCCCAGCCTGTCCCGGATGAGCTTGTCACTAAG
CTCCTGGGCAACCAGGCCACATTCAGCCCCATTGTCACCGTGGAGCCCCGGCGCCGGAAG
TTCCACCGCCCCATTGGGCTTCGGATCCCACTACCTCCTTCCTGGACCGACAACCCGAGG
GACAGCGGGGAGGGAGACACCACCAGCCTGCGCCTGCTTTGCAGCGTCATTGGAGGAACA
GACCAAGCCCAGTGGGAAGACATAACAGGAACCACCAAACTTGTATATGCCAACGAGTGC
GCCAACTTCACCACCAATGTCTCTGCCAGGTTTTGGCTGTCGGACTGTCCTCGGACTGCT
GAGGCTGTGAACTTTGCCACCCTGCTGTACAAAGAGCTCACTGCAGTGCCCTACATGGCC
AAATTCGTCATCTTTGCCAAGATGAATGACCCCCGAGAGGGGCGCCTGCGCTGCTACTGC
ATGACAGATGATAAAGTGGACAAGACCCTGGAGCAGCATGAGAACTTCGTGGAGGTGGCC
CGGAGCAGGGACATAGAGGTGTTGGAAGGAATGTCCCTGTTTGCAGAACTCTCTGGGAAC
CTGGTGCCTGTGAAGAAAGCTGCCCAGCAGCGGAGCTTCCACTTCCAGTCATTTCGGGAG
AACCGTCTGGCCATGCCTGTAAAGGTGAGGGACAGCAGTCGAGAGCCGGGAGGGTCCCTG
TCGTTTCTGCGCAAGGCGATGAAGTACGAGGACACCCAGCACATTCTCTGCCACCTGAAC
ATCACCATGCCCCCCTGCGCCAAGGGAAGTGGAGCCGAAGATAGGAGAAGGACCCCGACG
CCCCTGGCCCTGCGATACAGCATTCTCAGTGAGTCCACACCAGGTTCTCTCAGTGGGACA
GAGCAGGCAGAGATGAAGATGGCTGTTATCTCAGAGCACCTCGGTCTCAGCTGGGCAGAG
TTGGCCCGGGAGCTGCAGTTCAGTGTGGAAGACATCAACAGGATCCGAGTGGAAAATCCC
AACTCCCTGTTGGAGCAGAGTGTGGCCTTGCTGAACCTCTGGGTCATCCGTGAAGGCCAA
AACGCAAACATGGAGAATCTGTACACAGCCCTGCAGAGCATTGACCGTGGCGAGATCGTG
AACATGCTGGAGGGTTCCGGCCGACAGAGCCGCAACTTGAAGCCAGACAGGCGGCACACC
GACCGCGACTACTCGCTGTCACCCTCCCAGATGAATGGCCATCAGAGGGGGCAAGCCCGA
ATCACACATTCCCCCACCGTGAGTCAGGTGACGGAGAGGAGTCAGGACAGACTGCAGGAC
TGGGATGCAGACGGCTCGATTGTCTCATACCTGCAAGATGCTGCACAAGGTTCCTGGCAA
GAGGAGGTCACGCAAGGTCCACACTCATTCCAGGGAACAAGTACCATGACTGAAGGGCTA
GAGCCCGGTGGATCTCAGGAGTACGAGAAGGTCCTGGTGTCTGTAAGTGAGCACACGTGG
ACAGAACAGCCCGAGGCTGAGAGCTCCCAGGCCGACAGGGACCGGAGGCAGCAAGGCCAA
GAAGAGCAGGTGCAGGAGGCCAAGAACACCTTCACCCAAGTGGTGCAGGGGAATGAGTTT
CAGAATATTCCAGGGGAGCAGGTGACAGAGGAGCAATTCACGGATGAGCAGGGCAACATT
GTCACCAAGAAGATCATTCGCAAGGTGGTTCGACAGATAGACTTGTCCAGCGCCGATGCC
GCCCAGGAGCACGAGGAGGTGACTGTAGAGGGGCCCCTGGAGGATCCCAGTGAGCTGGAG
GTCGATATTGATTACTTTATGAAACACTCCAAGGATCACACCTCGACACCCAACCCCTGA
Restriction Sites Please inquire     
ACCN NM_020477
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_020477.2, NP_065210.2
RefSeq Size 7754 bp
RefSeq ORF 5160 bp
Locus ID 286
Cytogenetics 8p11.21
Protein Families Transmembrane
Gene Summary 'Ankyrins are a family of proteins that link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of all patients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving rise to different isoforms of ankyrin 1 have been described. Truncated muscle-specific isoforms of ankyrin 1 resulting from usage of an alternate promoter have also been identified. [provided by RefSeq, Dec 2008]'
Transcript Variant: This variant (2) lacks an in-frame segment near to the 3' coding region, compared to variant 1. The resulting isoform (2, also referred to as isoform 2.2) is therefore shorter than isoform 1.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.