Beta Arrestin 2 (ARRB2) (NM_199004) Human Untagged Clone

CAT#: SC307849

ARRB2 (untagged)-Human arrestin, beta 2 (ARRB2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ARRB2
• Synonyms: ARB2; ARR2; BARR2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_199004 edited
  GAATTCGGCACGAGGGGCGCGCGCACCATGGGGGAGAAACCCGGGACCAGGGTCTTCAAG
  AAGTCGAGCCCTAACTGCAAGCTCACCGTGTACTTGGGCAAGCGGGACTTCGTAGATCAC
  CTGGACAAAGTGGACCCTGTAGTGTTTGTGACCCTCACCTGCGCCTTCCGCTATGGCCGT
  GAAGACCTGGATGTGCTGGGCTTGTCCTTCCGCAAAGACCTGTTCATCGCCACCTACCAG
  GCCTTCCCCCCGGTGCCCAACCCACCCCGGCCCCCCACCCGCCTGCAGGACCGGCTGCTG
  AGGAAGCTGGGCCAGCATGCCCACCCCTTCTTCTTCACCATACCCCAGAATCTTCCATGC
  TCCGTCACACTGCAGCCAGGCCCAGAGGATACAGGAAAGGCCTGCGGCGTAGACTTTGAG
  ATTCGAGCCTTCTGTGCTAAATCACTAGAAGAGAAAAGCCACAAAAGGAACTCTGTGCGG
  CTGGTGATCCGAAAGGTGCAGTTCGCCCCGGAGAAACCCGGCCCCCAGCCTTCAGCCGAA
  ACCACACGCCACTTCCTCATGTCTGACCGGTCCCTGCACCTCGAGGCTTCCCTGGACAAG
  GAGCTGTACTACCATGGGGAGCCCCTCAATGTAAATGTCCACGTCACCAACAACTCCACC
  AAGACCGTCAAGAAGATCAAAGTCTCTGTGAGACAGTACGCCGACATCTGCCTCTTCAGC
  ACCGCCCAGTACAAGTGTCCTGTGGCTCAACTCGAACAAGATGACCAGGTATCTCCCAGC
  TCCACATTCTGTAAGGTGTACACCATAACCCCACTGCTCAGTGACAACCGGGAGAAGCGG
  GGTCTCGCCCTGGATGGGAAACTCAAGCACGAGGACACCAACCTGGCTTCCAGCACCATC
  GTGAAGGAGGGTGCCAACAAGGAGGTGCTGGGAATCCTGGTGTCCTACAGGGTCAAGGTG
  AAGCTGGTGGTGTCTCGAGGCGGGGATGTCTCTGTGGAGCTGCCTTTTGTTCTTATGCAC
  CCCAAGCCCCACGACCACATCCCCCTCCCCAGACCCCAGTCAGCCGCTCCGGAGACAGAT
  GTCCCTGTGGACACCAACCTCATTGAATTTGATACCAACTATGCCACAGATGATGACATT
  GTGTTTGAGGACTTTGCCCGGCTTCGGCTGAAGGGGATGAAGGATGACGACTATGATGAT
  CAACTCTGCTAGGAAGCGGGGTGGGAAGAAGGGAGGGGATGGGGTTGGGAGAGGTGAGGG
  CAGGATTAAGATCCCCACTGTCAATGGGGGATTGTCCCAGCCCCTCTTCCCTTCCCCTCA
  CCTGGAAGCTTCTTCAACCAATCCCTTCACACTCTCTCCCCCATCCCCCCAAGATACACA
  CTGGACCCTCTCTTGCTGAATGTGGGCATTAATTTTTTGACTGCAGCTCTGCTTCTCCAG
  CCCCGCCGTGGGTGGCAAGCTGTGTTCATACCTAAATTTTCTGGAAGGGGACAGTGAAAA
  GAGGAGTGACAGGAGGGAAAGGGGGAGACAAAACTCCTACTCTCAACCTCACACCAACAC
  CTCCCATTATCACTCTCTCTGCCCCCATTCCTTCAAGAGGAGACCCTTTGGGGACAAGGC
  CGTTTCTTTGTTTCTGAGCATAAAGAAGAAAATAAATCTTTTACTAAGAAAAAAAAAAAA
  AAAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_199004
• Insert Size: 1930 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_199004.1, NP_945355.1
• RefSeq Size: 1891 bp
• RefSeq ORF: 1185 bp
• Locus ID: 409
• Cytogenetics: 17p13.2
• Protein Families: Druggable Genome
• Protein Pathways: Chemokine signaling pathway, Endocytosis, MAPK signaling pathway, Olfactory transduction
• Gene Summary: 'Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 2, like arrestin beta 1, was shown to inhibit beta-adrenergic receptor function in vitro. It is expressed at high levels in the central nervous system and may play a role in the regulation of synaptic receptors. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012]'
  Transcript Variant: This variant (2) lacks an alternate in-frame exon and uses an alternate in-frame splice junction at the 3' end of another exon compared to variant 3. The resulting isoform (2) lacks two alternate internal segments compared to isoform 3.