DGKA (NM_201445) Human Untagged Clone

CAT#: SC308035

DGKA (untagged)-Human diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DGKA
• Synonyms: DAGK; DAGK1; DGK-alpha
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_201445, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCAAGGAGAGGGGCCTAATAAGCCCCAGTGATTTTGCCCAGCTGCAAAAATACATGGAATACTCCA
  CCAAAAAGGTCAGTGATGTCCTAAAGCTCTTCGAGGATGGCGAGATGGCTAAATATGTCCAAGGAGATGC
  CATTGGGTACGAGGGATTCCAGCAATTCCTGAAAATCTATCTCGAAGTGGATAATGTTCCCAGACACCTA
  AGCCTGGCACTGTTTCAATCCTTTGAGACTGGTCACTGCTTAAATGAGACAAATGTGACAAAAGATGTGG
  TGTGTCTCAATGATGTTTCCTGCTACTTTTCCCTTCTGGAGGGTGGTCGGCCAGAAGACAAGTTAGAATT
  CACCTTCAAGCTGTACGACACGGACAGAAATGGGATCCTGGACAGCTCAGAAGTGGACAAAATTATCCTA
  CAGATGATGCGAGTGGCTGAATACCTGGATTGGGATGTGTCTGAGCTGAGGCCGATTCTTCAGGAGATGA
  TGAAAGAGATTGACTATGATGGCAGTGGCTCTGTCTCTCAAGCTGAGTGGGTCCGGGCTGGGGCCACCAC
  CGTGCCACTGCTAGTGCTGCTGGGTCTGGAGATGACTCTGAAGGACGACGGACAGCACATGTGGAGGCCC
  AAGAGGTTCCCCAGACCAGTCTACTGCAATCTGTGCGAGTCAAGCATTGGTCTTGGCAAACAGGGACTGA
  GCTGTAACCTCTGTAAGTACACTGTTCACGACCAGTGTGCCATGAAAGCCCTGCCTTGTGAAGTCAGCAC
  CTATGCCAAGTCTCGGAAGGACATTGGTGTCCAATCACATGTGTGGGTGCGAGGAGGCTGTGAGTCCGGG
  CGCTGCGACCGCTGTCAGAAAAAGATCCGGATCTACCACAGTCTGACCGGGCTGCATTGTGTATGGTGCC
  ACCTAGAGATCCACGATGACTGCCTGCAAGCGGTGGGCCATGAGTGTGACTGTGGGCTGCTCCGGGATCA
  CATCCTGCCTCCATCTTCCATCTATCCCAGTGTCCTGGCCTCTGGACCGGATCGTAAAAATAGCAAAACA
  AGCCAGAAGACCATGGATGATTTAAATTTGAGCACCTCTGAGGCTCTGCGGATTGACCCTGTTCCTAACA
  CCCACCCACTTCTCGTCTTTGTCAATCCTAAGAGTGGCGGGAAGCAGGGGCAAAGGGTGCTCTGGAAGTT
  CCAGTATATATTAAACCCTCGACAGGTGTTCAACCTCCTAAAGGATGGTCCTGAGATAGGGCTCCGATTA
  TTCAAGGATGTTCCTGATAGCCGGATTTTGGTGTGTGGTGGAGACGGCACAGTAGGCTGGATTCTAGAGA
  CCATTGACAAAGCTAACTTGCCAGTTTTGCCTCCTGTTGCTGTGTTGCCCCTGGGTACTGGAAATGATCT
  GGCTCGATGCCTAAGATGGGGAGGAGGTTATGAAGGACAGAATCTGGCAAAGATCCTCAAGGATTTAGAG
  ATGAGTAAAGTGGTACATATGGATCGATGGTCTGTGGAGGTGATACCTCAACAAACTGAAGAAAAAAGTG
  ACCCAGTCCCCTTTCAAATCATCAATAACTACTTCTCTATTGGCGTGGATGCCTCTATTGCTCATCGATT
  CCACATCATGCGAGAGAAATATCCGGAGAAGTTCAACAGCAGAATGAAGAACAAGCTATGGTACTTCGAA
  TTTGCCACATCTGAATCCATCTTCTCAACATGCAAAAAGCTGGAGGAGTCTTTGACAGTTGAGATCTGTG
  GGAAACCGCTGGATCTGAGCAACCTGTCCCTAGAAGGCATCGCAGTGCTAAACATCCCTAGCATGCATGG
  TGGCTCCAACCTCTGGGGTGATACCAGGAGACCCCATGGGGATATCTATGGGATCAACCAGGCCTTAGGT
  GCTACAGCTAAAGTCATCACCGACCCTGATATCCTGAAAACCTGTGTACCAGACCTAAGTGACAAGAGAC
  TGGAAGTGGTTGGGCTGGAGGGTGCAATTGAGATGGGCCAAATCTATACCAAGCTCAAGAATGCTGGACG
  TCGGCTGGCCAAGTGCTCTGAGATCACCTTCCACACCACAAAAACCCTTCCCATGCAAATTGACGGAGAA
  CCCTGGATGCAGACGCCCTGTACAATCAAGATCACCCACAAGAACCAGATGCCCATGCTCATGGGCCCAC
  CCCCCCGCTCCACCAATTTCTTTGGCTTCTTGAGCTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_201445
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_201445.1, NP_958853.1
• RefSeq Size: 2769 bp
• RefSeq ORF: 2208 bp
• Locus ID: 1606
• Cytogenetics: 12q13.2
• Protein Families: Druggable Genome
• Protein Pathways: Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• Gene Summary: 'The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Several transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Apr 2017]'
  Transcript Variant: This variant (2), as well as variants 1 and 3-6, encodes isoform a.