Choline kinase alpha (CHKA) (NM_212469) Human Untagged Clone

CAT#: SC308631

CHKA (untagged)-Human choline kinase alpha (CHKA), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHKA
• Synonyms: CHK; CK; CKI; EK
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_212469 edited
  GCGGCCGCCCGCGCCTCCTCGGCCGCCTGTCGGGCATGAAAACCAAATTCTGCACCGGGG
  GCGAGGCGGAGCCCTCGCCGCTCGGGCTGCTGCTGAGCTGCGGTAGCGGCAGCGCGGCCC
  CGGCGCCCGGCGTGGGGCAGCAGCGCGACGCCGCCAGCGACCTCGAGTCCAAGCAGCTGG
  GCGGCCAACAGCCGCCGCTCGCGCTGCCCCCTCCGCCGCCGCTGCCGCTGCCGCTGCCGC
  TGCCCCAGCCCCCGCCGCCGCAGCCGCCCGCAGACGAGCAGCCGGAGCCCCGGACGCGGC
  GCAGGGCCTATCTGTGGTGCAAGGAGTTCCTGCCCGGCGCCTGGCGGGGCCTCCGCGAGG
  ACGAGTTCCACATCAGTGTCATCAGAGGCGGCCTTAGCAACATGCTGTTCCAGTGCTCCC
  TACCTGACACCACAGCCACCCTTGGTGATGAGCCTCGGAAAGTGCTCCTGCGGCTGTATG
  GAGCGATTTTGCAGGTGGGGGCTGAGGCCATGGTTCTGGAGAGCGTTATGTTTGCCATTC
  TCGCAGAGAGGTCACTTGGGCCAAAACTCTATGGCATCTTTCCCCAAGGCCGACTGGAGC
  AGTTCATCCCGAGCCGGCGATTAGATACTGAAGAATTAGGTTTGCCAGATATTTCTGCAG
  AAATCGCCGAGAAAATGGCTACATTTCATGGTATGAAAATGCCATTCAATAAGGAACCAA
  AATGGCTTTTTGGCACAATGGAAAAGTATCTAAAGGAAGTGCTGAGAATTAAATTTACTG
  AGGAATCCAGAATTAAAAAGCTCCACAAATTGCTCAGTTACAATCTGCCCTTGGAACTGG
  AAAACCTGAGATCATTGCTTGAATCTACTCCATCTCCAGTTGTATTTTGTCATAATGACT
  GTCAAGAAGGTAATATCTTGTTGCTGGAAGGCCGAGAGAATTCTGAAAAACAGAAACTGA
  TGCTCATTGATTTCGAATACAGCAGTTACAATTACAGGGGATTCGACATTGGAAATCACT
  TCTGTGAGTGGATGTATGATTATAGCTATGAAAAATACCCTTTTTTCAGAGCAAACATCC
  GGAAGTATCCCACCAAGAAACAACAGCTCCATTTTATTTCCAGTTACTTGCCTGCATTCC
  AAAATGACTTTGAAAACCTCAGTACTGAAGAAAAATCCATTATAAAAGAAGAAATGTTGC
  TTGAAGTTAATAGGTTTGCCCTTGCATCTCATTTCCTCTGGGGACAGTGGTCCATTGTAC
  AAGCCAAGATTTCATCTATTGAATTTGGGTACATGGACTACGCCCAAGCAAGGTTTGATG
  CCTATTTCCACCAGAAGAGGAAGCTTGGGGTGTGACTGTGGGGAGGACTCCATCCACCTC
  ATCACTGGACTGCATGGGGAGGCAGCAGAGCGGGGTCCCCTCTGTGCTTCGACTACTGCT
  CCTGTGGCAGGAGGCTTTGGGTGGCTCACTACTGAACACATGTGTATGATACTAAAGACG
  GTATTAAAATGGAGCGACGTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_212469
• Insert Size: 1600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to contain 3 SNPs compared with NM_212469.1.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_212469.1, NP_997634.1
• RefSeq Size: 2679 bp
• RefSeq ORF: 1320 bp
• Locus ID: 1119
• Cytogenetics: 11q13.2
• Protein Families: Druggable Genome
• Protein Pathways: Glycerophospholipid metabolism, Metabolic pathways
• Gene Summary: 'The major pathway for the biosynthesis of phosphatidylcholine occurs via the CDP-choline pathway. The protein encoded by this gene is the initial enzyme in the sequence and may play a regulatory role. The encoded protein also catalyzes the phosphorylation of ethanolamine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a.