HSPC302 (TBCK) (NM_033115) Human Untagged Clone

CAT#: SC308733

TBCK (untagged)-Human TBC1 domain containing kinase (TBCK), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TBCK
• Synonyms: HSPC302; IHPRF3; TBCKL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_033115, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTTTCCCCTGAAGGACGCTGAAATGGGAGCCTTTACCTTCTTTGCCTCGGCTCTGCCACATGATGTTT
  GTGGAAGCAATGGACTTCCTCTCACACCAAATTCCATCAAAATTTTAGGGCGCTTTCAAATCCTTAAAAC
  CATCACCCATCCCAGACTCTGCCAGTATGTGGATATTTCTAGGGGAAAGCATGAACGACTAGTGGTCGTG
  GCTGAACATTGTGAACGTAGTCTGGAAGACTTGCTTCGAGAAAGGAAACCTGTGAGGTATCCCTCGTACT
  TGGCCCCTGAGGTAATTGCACAGGGAATTTTCAAAACCACTGATCACATGCCAAGTAAAAAACCATTGCC
  TTCTGGCCCCAAATCAGATGTATGGTCTCTTGGAATCATTTTATTTGAGCTTTGTGTGGGAAGAAAATTA
  TTTCAGAGCTTGGATATTTCTGAAAGACTAAAATTTTTGCTTACTTTGGATTGTGTAGATGACACTTTAA
  TAGTTCTGGCTGAAGAGCATGGTTGTTTGGACATTATAAAGGAGCTTCCTGAAACTGTGATAGATCTTTT
  GAATAAGTGCCTTACCTTCCATCCTTCTAAGAGGCCAACCCCAGATGAATTAATGAAGGACAAAGTATTC
  AGTGAGGTATCACCTTTATATACCCCCTTTACCAAACCTGCCAGTCTGTTTTCATCTTCTCTGAGATGTG
  CTGATTTAACTCTGCCTGAGGATATCAGTCAGTTGTGTAAAGATATAAATAATGATTACCTGGCAGAAAG
  ATCTATTGAAGAAGTGTATTACCTTTGGTGTTTGGCTGGAGGTGACTTGGAGAAAGAGCTTGTCAACAAG
  GAAATCATTCGATCCAAACCACCTATCTGCACACTCCCCAATTTTCTCTTTGAGGATGGTGAAAGCTTTG
  GACAAGGTCGAGATAGAAGCTCGCTTTTAGATGATACCACTGTGACATTGTCGTTATGCCAGCTAAGAAA
  TAGATTGAAAGATGTTGGTGGAGAAGCATTTTACCCATTACTTGAAGATGACCAGTCTAATTTACCTCAT
  TCAAACAGCAATAATGAGTTGTCTGCAGCTGCCACGCTCCCTTTAATCATCAGAGAGAAGGATACAGAGT
  ACCAACTAAATAGAATTATTCTCTTCGACAGGCTGCTAAAGGCTTATCCATATAAAAAAAACCAAATCTG
  GAAAGAAGCAAGAGTTGACATTCCTCCTCTTATGAGAGGTTTAACCTGGGCTGCTCTTCTGGGAGTTGAG
  GGAGCTATTCATGCCAAGTACGATGCAATTGATAAAGACACTCCAATTCCTACAGATAGACAAATTGAAG
  TGGATATTCCTCGCTGTCATCAGTACGATGAACTGTTATCATCACCAGAAGGTCATGCAAAATTTAGGCG
  TGTATTAAAAGCCTGGGTAGTGTCTCATCCTGATCTTGTGTATTGGCAAGGTCTTGACTCACTTTGTGCT
  CCATTCCTATATCTAAACTTCAATAATGAAGCCTTGGCTTATGCATGTATGTCTGCTTTTATTCCCAAAT
  ACCTGTATAACTTCTTCTTAAAAGACAACTCACATGTAATACAAGAGTATCTGACTGTCTTCTCTCAGAT
  GATTGCATTTCATGATCCAGAGCTGAGTAATCATCTCAATGAGATTGGTTTCATTCCAGATCTCTATGCC
  ATCCCTTGGTTTCTTACCATGTTTACTCATGTATTTCCACTACACAAAATTTTCCACCTCTGGGATACCT
  TACTACTTGGGAATTCCTCTTTCCCATTCTGTATTGGAGTAGCAATTCTTCAGCAGCTGCGGGACCGGCT
  TTTGGCTAATGGCTTTAATGAGTGTATTCTTCTCTTCTCCGATTTACCAGAAATTGACATTGAACGCTGT
  GTGAGAGAATCTATCAACCTGTTTTGTTGGACTCCTAAAAGTGCTACTTACAGACAGCATGCTCAACCTC
  CAAAGCCATCTTCTGACAGCAGTGGAGGCAGAAGTTCGGCACCTTATTTCTCTGCTGAGTGTCCAGATCC
  TCCAAAGACAGATCTGTCAAGAGAATCCATCCCATTAAATGACCTGAAGTCAGAAGTATCACCACGGATT
  TCAGCAGAGGACCTGATTGACTTGTGTGAGCTCACAGTGACAGGCCACTTCAAAACACCCAGCAAGAAAA
  CAAAGTCCAGTAAACCAAAGCTCCTGGTGGTTGACATCCGGAATAGTGAAGACTTTATTCGTGGTCACAT
  TTCAGGAAGCATCAACATTCCATTCAGTGCTGCCTTCACTGCAGAAGGGGAGCTTACCCAGGGCCCTTAC
  ACTGCTATGCTCCAGAACTTCAAAGGGAAGGTCATTGTCATCGTGGGGCATGTGGCAAAACACACAGCTG
  AGTTTGCAGCTCACCTTGTGAAGATGAAATATCCAAGAATCTGTATTCTAGATGGTGGCATTAATAAAAT
  AAAGCCAACAGGCCTCCTCACCATCCCATCTCCTCAAATATGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_033115
• ORF Size: 2493 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_033115.4, NP_149106.2
• RefSeq Size: 5120
• RefSeq ORF: 2493
• Locus ID: 93627
• Domains: TBC, pkinase, RHOD, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a protein that contains a protein kinase domain, a Rhodanase-like domain and the Tre-2/Bub2/Cdc16 (TBC) domain. The encoded protein is thought to play a role in actin organization, cell growth and cell proliferation by regulating the mammalian target of the rapamycin (mTOR) signaling pathway. This protein may also be involved in the transcriptional regulation of the components of the mTOR complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
  Transcript Variant: This variant (4) lacks two in-frame exons in the 5' coding region compared to variant 1. This results in a shorter protein (isoform c) compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.