TBX5 (NM_080717) Human Untagged Clone

CAT#: SC309083

TBX5 (untagged)-Human T-box 5 (TBX5), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TBX5
• Synonyms: HOS
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_080717, the custom clone sequence may differ by one or more nucleotides
  ATGGAGGGAATCAAAGTGTTTCTCCATGAAAGAGAACTGTGGCTAAAATTCCACGAAGTG
  GGCACGGAAATGATCATAACCAAGGCTGGAAGGCGGATGTTTCCCAGTTACAAAGTGAAG
  GTGACGGGCCTTAATCCCAAAACGAAGTACATTCTTCTCATGGACATTGTACCTGCCGAC
  GATCACAGATACAAATTCGCAGATAATAAATGGTCTGTGACGGGCAAAGCTGAGCCCGCC
  ATGCCTGGCCGCCTGTACGTGCACCCAGACTCCCCCGCCACCGGGGCGCATTGGATGAGG
  CAGCTCGTCTCCTTCCAGAAACTCAAGCTCACCAACAACCACCTGGACCCATTTGGGCAT
  ATTATTCTAAATTCCATGCACAAATACCAGCCTAGATTACACATCGTGAAAGCGGATGAA
  AATAATGGATTTGGCTCAAAAAATACAGCGTTCTGCACTCACGTCTTTCCTGAGACTGCG
  TTTATAGCAGTGACTTCCTACCAGAACCACAAGATCACGCAATTAAAGATTGAGAATAAT
  CCCTTTGCCAAAGGATTTCGGGGCAGTGATGACATGGAGCTGCACAGAATGTCAAGAATG
  CAAAGTAAAGAATATCCCGTGGTCCCCAGGAGCACCGTGAGGCAAAAAGTGGCCTCCAAC
  CACAGTCCTTTCAGCAGCGAGTCTCGAGCTCTCTCCACCTCATCCAATTTGGGGTCCCAA
  TACCAGTGTGAGAATGGTGTTTCCGGCCCCTCCCAGGACCTCCTGCCTCCACCCAACCCA
  TACCCACTGCCCCAGGAGCATAGCCAAATTTACCATTGTACCAAGAGGAAAGAGGAAGAA
  TGTTCCACCACAGACCATCCCTATAAGAAGCCCTACATGGAGACATCACCCAGTGAAGAA
  GATTCCTTCTACCGCTCTAGCTATCCACAGCAGCAGGGCCTGGGTGCCTCCTACAGGACA
  GAGTCGGCACAGCGGCAAGCTTGCATGTATGCCAGCTCTGCGCCCCCCAGCGAGCCTGTG
  CCCAGCCTAGAGGACATCAGCTGCAACACGTGGCCAAGCATGCCTTCCTACAGCAGCTGC
  ACCGTCACCACCGTGCAGCCCATGGACAGGCTACCCTACCAGCACTTCTCCGCTCACTTC
  ACCTCGGGGCCCCTGGTCCCTCGGCTGGCTGGCATGGCCAACCATGGCTCCCCACAGCTG
  GGAGAGGGAATGTTCCAGCACCAGACCTCCGTGGCCCACCAGCCTGTGGTCAGGCAGTGT
  GGGCCTCAGACTGGCCTGCAGTCCCCTGGCACCCTTCAGCCCCCTGAGTTCCTCTACTCT
  CATGGCGTGCCAAGGACTCTATCCCCTCATCAGTACCACTCTGTGCACGGAGTTGGCATG
  GTGCCAGAGTGGAGCGACAATAGCTAA
  
• Restriction Sites: Please inquire
• ACCN: NM_080717
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080717.2, NP_542448.1
• RefSeq Size: 3736 bp
• RefSeq ORF: 1407 bp
• Locus ID: 6910
• Cytogenetics: 12q24.21
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity. Mutations in this gene have been associated with Holt-Oram syndrome, a developmental disorder affecting the heart and upper limbs. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (3) lacks the exon containing the translation start site compared to transcript variant 1. The resulting isoform (3) is shorter at the N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.