ANK1 (NM_000037) Human Untagged Clone

CAT#: SC309443

ANK1 (untagged)-Human ankyrin 1, erythrocytic (ANK1), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ANK1
• Synonyms: ANK; SPH1; SPH2
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000037, the custom clone sequence may differ by one or more nucleotides
  ATGCCCTATTCTGTGGGCTTCCGCGAAGCCGATGCTGCTACCAGCTTTCTGAGAGCAGCA
  AGATCAGGTAACTTGGACAAAGCTTTGGATCACCTGCGGAATGGGGTAGATATTAACACC
  TGTAACCAGAATGGGTTGAATGGCTTGCATCTGGCTTCTAAGGAAGGCCATGTGAAAATG
  GTGGTTGAACTTCTGCACAAAGAAATCATTCTAGAAACGACAACCAAGAAGGGGAACACG
  GCCCTGCACATCGCTGCTCTAGCCGGGCAGGATGAGGTGGTCCGGGAGCTTGTCAACTAT
  GGAGCCAACGTCAACGCCCAGTCACAGAAAGGTTTTACACCCCTGTACATGGCAGCACAA
  GAGAACCACTTGGAAGTGGTTAAGTTTTTACTGGAAAATGGAGCTAACCAGAATGTAGCC
  ACAGAAGACGGCTTCACGCCTCTGGCGGTAGCCCTGCAGCAGGGCCATGAGAACGTCGTC
  GCGCACCTCATCAACTACGGCACCAAGGGGAAGGTGCGCCTCCCGGCCCTGCACATCGCG
  GCCCGCAACGACGACACGCGCACGGCTGCGGTGCTGCTGCAGAACGACCCCAACCCGGAC
  GTGCTTTCCAAGACGGGATTCACGCCCCTGCACATTGCGGCTCACTACGAGAACCTCAAC
  GTGGCCCAGTTGCTCCTCAACAGAGGAGCCAGCGTCAATTTCACACCACAGAACGGCATC
  ACGCCACTGCACATCGCCTCCCGCAGGGGCAACGTGATCATGGTGCGGCTGCTGCTGGAT
  CGGGGAGCCCAGATAGAAACCAAGACCAAGGACGAATTGACACCTCTCCACTGTGCAGCT
  CGAAATGGGCACGTGCGAATCTCAGAGATCCTGCTGGACCACGGGGCACCAATCCAAGCC
  AAAACCAAGAACGGCCTGTCCCCAATTCACATGGCGGCTCAGGGAGACCACCTCGACTGT
  GTCCGGCTCCTGTTGCAATACGACGCAGAGATAGACGACATCACCCTGGACCACCTGACC
  CCACTCCACGTGGCTGCCCACTGTGGACACCACAGGGTGGCTAAGGTCCTTCTGGATAAA
  GGGGCCAAACCCAACTCCAGAGCCCTGAATGGCTTTACCCCCTTACACATCGCCTGCAAA
  AAGAACCACGTCCGTGTCATGGAGCTGCTGCTGAAGACGGGAGCCTCGATCGACGCGGTC
  ACCGAGTCTGGCCTGACACCTCTCCACGTGGCCTCCTTCATGGGGCACCTTCCCATCGTG
  AAGAACCTCCTGCAGCGGGGGGCGTCGCCCAACGTCTCCAACGTGAAAGTGGAGACCCCG
  CTACACATGGCAGCCAGAGCCGGGCACACGGAAGTGGCCAAATATTTACTCCAGAACAAA
  GCCAAAGTCAATGCCAAGGCCAAGGATGACCAGACCCCACTTCACTGTGCAGCTCGCATC
  GGCCACACAAACATGGTGAAGCTCCTGCTGGAAAATAACGCCAACCCCAACCTGGCCACC
  ACCGCCGGGCACACCCCCCTGCACATTGCAGCCCGTGAGGGCCATGTGGAAACAGTCCTG
  GCCCTTCTGGAAAAGGAAGCATCCCAGGCCTGCATGACCAAGAAAGGATTTACCCCTCTG
  CACGTGGCGGCCAAGTACGGGAAGGTGCGGGTGGCAGAGCTGCTGCTGGAGCGGGACGCA
  CACCCGAATGCTGCCGGAAAAAATGGCCTGACCCCCCTGCACGTGGCCGTCCATCACAAC
  AACCTGGACATCGTCAAGCTGCTGCTTCCCCGGGGCGGCTCCCCGCACAGCCCTGCCTGG
  AATGGCTACACCCCTTTGCACATCGCTGCCAAGCAGAACCAGGTGGAGGTGGCCCGTAGT
  CTGCTGCAGTATGGGGGCTCAGCAAACGCCGAGTCGGTGCAAGGTGTGACGCCCCTTCAC
  CTGGCCGCCCAGGAGGGCCACGCAGAGATGGTGGCTCTGCTGCTCTCGAAACAAGCCAAT
  GGCAACCTGGGGAACAAGAGCGGACTCACTCCCCTCCATCTGGTAGCACAAGAAGGCCAC
  GTTCCAGTGGCAGATGTGCTGATCAAACACGGCGTCATGGTGGACGCCACCACCCGGATG
  GGCTACACTCCCCTCCATGTGGCCAGTCACTATGGAAACATCAAGCTGGTGAAGTTTCTG
  CTGCAGCACCAGGCAGATGTCAATGCCAAGACCAAGCTAGGATACAGCCCCCTGCACCAG
  GCAGCCCAGCAGGGACACACAGACATCGTGACTCTGCTTCTGAAAAACGGTGCTTCCCCA
  AACGAGGTCAGCTCGGATGGAACCACACCTCTGGCCATAGCCAAGCGCTTGGGCTACATT
  TCTGTCACCGACGTGCTCAAGGTCGTCACGGATGAAACCAGTTTCGTGTTAGTCAGTGAT
  AAGCATCGAATGAGTTTCCCTGAGACAGTTGATGAGATCCTGGATGTCTCGGAAGATGAA
  GGGGAAGAACTCATCAGCTTCAAGGCTGAGAGGCGGGATTCCAGGGATGTTGATGAAGAG
  AAGGAGCTGCTGGATTTTGTGCCGAAGCTAGACCAAGTGGTGGAATCTCCAGCCATCCCC
  AGGATTCCCTGTGCCATGCCTGAGACAGTGGTGATCAGGTCAGAAGAGCAGGAGCAGGCA
  TCTAAAGAGTATGATGAGGACTCCCTCATCCCCAGCAGCCCGGCCACCGAGACCTCAGAC
  AACATCAGCCCGGTGGCCAGCCCGGTGCATACAGGGTTTCTGGTGAGCTTCATGGTTGAC
  GCCCGGGGTGGTTCCATGAGAGGAAGTCGCCACAACGGCCTGCGAGTGGTGATCCCGCCA
  CGGACGTGCGCAGCGCCCACCCGCATCACCTGCCGCCTGGTCAAGCCCCAGAAGCTCAGC
  ACGCCGCCCCCACTGGCCGAGGAGGAGGGCCTGGCCAGCAGGATCATAGCACTGGGGCCC
  ACGGGGGCACAGTTCCTGAGCCCTGTAATCGTGGAGATCCCGCACTTTGCCTCCCATGGC
  CGTGGAGACCGCGAGCTCGTGGTTCTGAGGAGCGAAAACGGCTCCGTGTGGAAGGAGCAC
  AGGAGCCGCTATGGAGAGAGCTACCTGGATCAGATCCTCAACGGGATGGACGAAGAGCTG
  GGGAGCCTGGAGGAGCTAGAGAAGAAGAGGGTGTGCCGAATCATCACCACCGACTTCCCG
  CTGTACTTCGTGATCATGTCACGGCTCTGCCAGGACTACGACACCATCGGTCCCGAAGGG
  GGCTCCCTGAAGAGCAAGCTGGTGCCCCTGGTACAGGCAACGTTCCCGGAGAATGCCGTC
  ACCAAGAGAGTGAAGCTGGCTCTGCAGGCCCAGCCTGTCCCGGATGAGCTTGTCACTAAG
  CTCCTGGGCAACCAGGCCACATTCAGCCCCATTGTCACCGTGGAGCCCCGGCGCCGGAAG
  TTCCACCGCCCCATTGGGCTTCGGATCCCACTACCTCCTTCCTGGACCGACAACCCGAGG
  GACAGCGGGGAGGGAGACACCACCAGCCTGCGCCTGCTTTGCAGCGTCATTGGAGGAACA
  GACCAAGCCCAGTGGGAAGACATAACAGGAACCACCAAACTTGTATATGCCAACGAGTGC
  GCCAACTTCACCACCAATGTCTCTGCCAGGTTTTGGCTGTCGGACTGTCCTCGGACTGCT
  GAGGCTGTGAACTTTGCCACCCTGCTGTACAAAGAGCTCACTGCAGTGCCCTACATGGCC
  AAATTCGTCATCTTTGCCAAGATGAATGACCCCCGAGAGGGGCGCCTGCGCTGCTACTGC
  ATGACAGATGATAAAGTGGACAAGACCCTGGAGCAGCATGAGAACTTCGTGGAGGTGGCC
  CGGAGCAGGGACATAGAGGTGTTGGAAGGAATGTCCCTGTTTGCAGAACTCTCTGGGAAC
  CTGGTGCCTGTGAAGAAAGCTGCCCAGCAGCGGAGCTTCCACTTCCAGTCATTTCGGGAG
  AACCGTCTGGCCATGCCTGTAAAGGTGAGGGACAGCAGTCGAGAGCCGGGAGGGTCCCTG
  TCGTTTCTGCGCAAGGCGATGAAGTACGAGGACACCCAGCACATTCTCTGCCACCTGAAC
  ATCACCATGCCCCCCTGCGCCAAGGGAAGTGGAGCCGAAGATAGGAGAAGGACCCCGACG
  CCCCTGGCCCTGCGATACAGCATTCTCAGTGAGTCCACACCAGGTTCTCTCAGTGGGACA
  GAGCAGGCAGAGATGAAGATGGCTGTTATCTCAGAGCACCTCGGTCTCAGCTGGGCAGAG
  TTGGCCCGGGAGCTGCAGTTCAGTGTGGAAGACATCAACAGGATCCGAGTGGAAAATCCC
  AACTCCCTGTTGGAGCAGAGTGTGGCCTTGCTGAACCTCTGGGTCATCCGTGAAGGCCAA
  AACGCAAACATGGAGAATCTGTACACAGCCCTGCAGAGCATTGACCGTGGCGAGATCGTG
  AACATGCTGGAGGGTTCCGGCCGACAGAGCCGCAACTTGAAGCCAGACAGGCGGCACACC
  GACCGCGACTACTCGCTGTCACCCTCCCAGATGAATGGTTACTCCTCACTGCAGGACGAG
  CTGCTGTCCCCTGCCTCCCTGGGCTGTGCACTTTCCTCTCCGCTACGTGCAGACCAGTAC
  TGGAATGAGGTGGCCGTCCTAGACGCCATCCCCTTGGCGGCCACGGAGCATGACACCATG
  CTGGAGATGTCTGACATGCAGGTGTGGTCTGCGGGCCTCACGCCTTCTCTGGTCACTGCT
  GAGGACTCCTCTCTGGAGTGTAGCAAGGCTGAGGACTCTGATGCCACAGGTCACGAGTGG
  AAGTTGGAGGGGGCACTCTCAGAGGAACCGCGGGGCCCCGAGTTGGGCTCTCTGGAACTT
  GTGGAGGACGACACAGTGGATTCAGATGCCACAAATGGCCTTATCGATTTGCTTGAACAG
  GAGGAAGGTCAGAGGTCAGAAGAGAAGCTGCCAGGTTCTAAGAGGCAGGATGACGCGACA
  GGTGCAGGGCAGGACTCAGAGAATGAAGTGTCTCTTGTTTCAGGCCATCAGAGGGGGCAA
  GCCCGAATCACACATTCCCCCACCGTGAGTCAGGTGACGGAGAGGAGTCAGGACAGACTG
  CAGGACTGGGATGCAGACGGCTCGATTGTCTCATACCTGCAAGATGCTGCACAAGGTTCC
  TGGCAAGAGGAGGTCACGCAAGGTCCACACTCATTCCAGGGAACAAGTACCATGACTGAA
  GGGCTAGAGCCCGGTGGATCTCAGGAGTACGAGAAGGTCCTGGTGTCTGTAAGTGAGCAC
  ACGTGGACAGAACAGCCCGAGGCTGAGAGCTCCCAGGCCGACAGGGACCGGAGGCAGCAA
  GGCCAAGAAGAGCAGGTGCAGGAGGCCAAGAACACCTTCACCCAAGTGGTGCAGGGGAAT
  GAGTTTCAGAATATTCCAGGGGAGCAGGTGACAGAGGAGCAATTCACGGATGAGCAGGGC
  AACATTGTCACCAAGAAGATCATTCGCAAGGTGGTTCGACAGATAGACTTGTCCAGCGCC
  GATGCCGCCCAGGAGCACGAGGAGGTGGAGCTGAGAGGGAGTGGCCTACAGCCGGACCTG
  ATAGAGGGCAGGAAGGGGGCGCAGATAGTGAAGCGGGCCAGCCTGAAAAGGGGGAAACAG
  TGA
  
• Restriction Sites: Please inquire
• ACCN: NM_000037
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000037.3, NP_000028.3
• RefSeq Size: 8300 bp
• RefSeq ORF: 5643 bp
• Locus ID: 286
• Cytogenetics: 8p11.21
• Domains: DEATH, ZU5, ANK
• Protein Families: Transmembrane
• Gene Summary: 'Ankyrins are a family of proteins that link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of all patients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving rise to different isoforms of ankyrin 1 have been described. Truncated muscle-specific isoforms of ankyrin 1 resulting from usage of an alternate promoter have also been identified. [provided by RefSeq, Dec 2008]'
  Transcript Variant: This variant (3) has an alternate segment in the 3' coding region, which includes a stop codon, compared to variant 1. The resulting isoform (3) has a distinct and one aa shorter C-terminus, compared to isoform 1.