MYO1E (NM_004998) Human Untagged Clone

CAT#: SC309978

MYO1E (untagged)-Human myosin IE (MYO1E)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MYO1E
• Synonyms: FSGS6; HuncM-IC; MYO1C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004998, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGAAGCAAAGGTGTCTACCAGTACCACTGGCAAAGCCACAATGTCAAGCACAGTGGTGTGGACGACA
  TGGTGCTACTGTCCAAGATCACAGAGAACTCCATCGTGGAGAATCTGAAGAAGAGATACATGGATGACTA
  CATTTTTACATATATAGGATCTGTATTAATCTCAGTCAACCCTTTCAAGCAGATGCCATATTTTGGGGAA
  AAGGAAATTGAAATGTACCAAGGAGCGGCACAGTATGAAAACCCACCACATATCTATGCCCTTGCAGATA
  ATATGTACAGAAACATGATCATTGACAGAGAGAACCAGTGCGTCATTATCAGTGGTGAAAGTGGTGCTGG
  AAAAACAGTGGCTGCCAAATATATCATGAGCTACATCTCCAGAGTGTCTGGAGGAGGGACCAAAGTCCAG
  CACGTGAAGGACATTATCCTGCAGTCCAACCCGCTGCTGGAGGCCTTCGGGAACGCCAAGACCGTCCGGA
  ACAACAACTCCAGCCGATTTGGAAAATACTTTGAAATCCAGTTCAGTCCAGGTGGGGAACCAGATGGTGG
  AAAGATCTCCAACTTCCTTCTGGAAAAATCTAGGGTGGTGATGAGGAACCCAGGAGAGCGGAGTTTTCAC
  ATATTTTACCAGCTCATCGAGGGCGCCTCTGCAGAGCAGAAACACAGCCTTGGCATCACCAGCATGGACT
  ATTATTACTACCTGAGCCTCTCGGGCTCATACAAGGTTGATGACATTGACGACAGGCGGGAGTTTCAGGA
  AACTCTGCACGCCATGAATGTGATTGGGATCTTTGCAGAAGAGCAAACGCTGGTGTTGCAGATAGTGGCG
  GGTATTCTCCACCTGGGAAACATCAGCTTCAAAGAAGTTGGCAACTACGCGGCTGTGGAGAGTGAAGAGT
  TTTTAGCTTTTCCTGCATATCTGCTAGGGATAAACCAGGACCGGTTGAAAGAAAAGCTAACAAGCCGGCA
  GATGGATAGCAAGTGGGGAGGCAAATCCGAATCCATCCACGTGACCCTCAACGTAGAGCAGGCCTGTTAC
  ACCCGGGATGCGCTCGCCAAGGCCCTGCACGCCCGGGTCTTTGATTTCTTGGTAGATTCCATCAATAAAG
  CCATGGAGAAAGACCATGAAGAATACAACATTGGCGTCCTAGACATCTATGGCTTTGAAATATTCCAGAA
  AAATGGCTTTGAACAGTTTTGTATCAATTTTGTTAATGAAAAACTGCAGCAGATTTTTATTGAACTGACA
  TTAAAGGCAGAACAGGAAGAATATGTTCAAGAGGGAATAAGATGGACACCCATTGAGTACTTTAATAATA
  AAATCGTATGTGACCTCATAGAGAACAAAGTGAACCCTCCTGGCATCATGAGCATCCTGGATGACGTGTG
  CGCCACGATGCATGCGGTGGGTGAGGGGGCAGATCAGACGCTGCTCCAGAAACTTCAGATGCAGATTGGG
  AGTCATGAGCACTTCAACAGTTGGAACCAAGGCTTCATCATTCATCATTATGCTGGGAAGGTATCCTATG
  ACATGGATGGCTTTTGTGAAAGGAACCGGGATGTGCTTTTTATGGATCTCATCGAGCTTATGCAGAGCAG
  CGAGCTGCCTTTCATAAAGTCTTTATTTCCGGAAAATCTGCAGGCTGACAAGAAAGGGCGCCCAACTACT
  GCCGGAAGCAAAATAAAGAAACAAGCCAATGACCTTGTGAGCACCCTGATGAAATGTACGCCCCACTACA
  TTCGCTGCATCAAGCCAAACGAAACCAAGAAGCCCAGAGACTGGGAGGAAAGCAGGGTAAAGCATCAAGT
  CGAATATTTGGGTCTGAAAGAGAACATTCGAGTGAGAAGAGCTGGCTATGCCTATCGGCGCATCTTCCAA
  AAATTCCTACAGAGGTATGCCATTCTGACCAAAGCCACCTGGCCTTCTTGGCAGGGAGAGGAGAAGCAAG
  GCGTCCTGCACCTGCTGCAGTCGGTCAACATGGACAGCGACCAGTTCCAGCTGGGGAGGAGTAAAGTGTT
  CATCAAAGCCCCCGAGTCTCTATTTCTTTTAGAAGAGATGAGAGAGAGAAAGTATGATGGGTATGCTCGA
  GTGATACAGAAATCATGGAGGAAATTCGTGGCCCGGAAGAAATACGTTCAAATGAGAGAAGAAGCCTCAG
  ACCTCTTATTGAACAAGAAGGAGAGAAGGAGAAACAGTATTAACAGGAACTTTATAGGGGATTATATTGG
  GATGGAAGAGCACCCAGAACTCCAGCAGTTCGTGGGCAAGAGGGAGAAGATTGATTTCGCAGACACAGTC
  ACCAAGTATGACAGGAGGTTCAAGGGTGTAAAGCGAGACCTGCTCCTTACCCCAAAGTGCTTGTACTTAA
  TCGGACGAGAAAAAGTCAAACAGGGCCCAGACAAGGGCCTGGTGAAAGAAGTCCTGAAGCGGAAAATCGA
  GATAGAACGGATCTTGTCTGTGTCCCTCAGTACTATGCAGGATGACATTTTTATTCTCCATGAGCAAGAG
  TATGACAGTTTGCTTGAATCTGTCTTCAAAACTGAATTCCTAAGCCTCTTAGCAAAGCGTTACGAGGAGA
  AGACCCAGAAGCAACTACCTCTGAAATTCAGCAATACGCTTGAACTGAAGTTGAAAAAGGAAAACTGGGG
  CCCCTGGAGTGCAGGGGGCTCCCGGCAAGTGCAGTTCCACCAAGGGTTTGGGGACCTGGCTGTCCTCAAG
  CCCAGTAACAAAGTGCTGCAGGTCAGCATCGGACCTGGACTGCCCAAGAACTCCCGTCCTACCAGAAGGA
  ACACTACCCAAAATACAGGTTATTCCAGTGGGACTCAAAATGCCAACTACCCAGTGAGAGCTGCCCCTCC
  TCCCCCAGGATACCATCAGAACGGAGTCATCAGAAACCAGTATGTGCCATATCCCCATGCTCCTGGAAGC
  CAGAGGTCCAATCAGAAAAGCCTGTACACCTCCATGGCCCGCCCGCCCTTGCCTCGGCAGCAGTCTACCA
  GTTCAGACCGAGTGTCACAGACGCCAGAGAGCCTGGATTTCCTCAAGGTCCCGGACCAGGGAGCTGCAGG
  GGTCAGGAGACAAACAACCAGTCGGCCTCCCCCAGCAGGGGGCAGACCCAAGCCCCAGCCCAAGCCCAAG
  CCTCAGGTGCCACAGTGCAAGGCTTTGTATGCCTATGACGCTCAGGACACAGACGAACTCAGCTTTAATG
  CCAATGACATTATTGATATTATCAAAGAAGATCCTTCTGGCTGGTGGACGGGTCGACTACGAGGCAAGCA
  GGGCCTGTTCCCCAACAACTATGTGACCAAGATCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_004998
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004998.3, NP_004989.2
• RefSeq Size: 5125 bp
• RefSeq ORF: 3327 bp
• Locus ID: 4643
• Cytogenetics: 15q22.2
• Domains: IQ, SH3, myosin_head
• Gene Summary: 'This gene encodes a member of the nonmuscle class I myosins which are a subgroup of the unconventional myosin protein family. The unconventional myosin proteins function as actin-based molecular motors. Class I myosins are characterized by a head (motor) domain, a regulatory domain and a either a short or long tail domain. Among the class I myosins, this protein is distinguished by a long tail domain that is involved in crosslinking actin filaments. This protein localizes to the cytoplasm and may be involved in intracellular movement and membrane trafficking. Mutations in this gene are the cause of focal segmental glomerulosclerosis-6. This gene has been referred to as myosin IC in the literature but is distinct from the myosin IC gene located on chromosome 17. [provided by RefSeq, Jan 2012]'