CCN1 (CYR61) (NM_001554) Human Untagged Clone

CAT#: SC310465

CYR61 (untagged)-Human cysteine-rich, angiogenic inducer, 61 (CYR61)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CCN1
• Synonyms: CYR61; GIG1; IGFBP10
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC310465 sequence for NM_001554 edited (data generated by NextGen Sequencing)
  ATGAGCTCCCGCATCGCCAGGGCGCTCGCCTTAGTCGTCACCCTTCTCCACTTGACCAGG
  CTGGCGCTCTCCACCTGCCCCGCTGCCTGCCACTGCCCCCTGGAGGCGCCCAAGTGCGCG
  CCGGGAGTCGGGCTGGTCCGGGACGGCTGCGGCTGCTGTAAGGTCTGCGCCAAGCAGCTC
  AACGAGGACTGCAGCAAAACGCAGCCCTGCGACCACACCAAGGGGCTGGAATGCAACTTC
  GGCGCCAGCTCCACCGCTCTGAAGGGGATCTGCAGAGCTCAGTCAGAGGGCAGACCCTGT
  GAATATAACTCCAGAATCTACCAAAACGGGGAAAGTTTCCAGCCCAACTGTAAACATCAG
  TGCACATGTATTGATGGCGCCGTGGGCTGCATTCCTCTGTGTCCCCAAGAACTATCTCTC
  CCCAACTTGGGCTGTCCCAACCCTCGGCTGGTCAAAGTTACCGGGCAGTGCTGCGAGGAG
  TGGGTCTGTGACGAGGATAGTATCAAGGACCCCATGGAGGACCAGGACGGCCTCCTTGGC
  AAGGAGCTGGGATTCGATGCCTCCGAGGTGGAGTTGACGAGAAACAATGAATTGATTGCA
  GTTGGAAAAGGCAGCTCACTGAAGCGGCTCCCTGTTTTTGGAATGGAGCCTCGCATCCTA
  TACAACCCTTTACAAGGCCAGAAATGTATTGTTCAAACAACTTCATGGTCCCAGTGCTCA
  AAGACCTGTGGAACTGGTATCTCCACACGAGTTACCAATGACAACCCTGAGTGCCGCCTT
  GTGAAAGAAACCCGGATTTGTGAGGTGCGGCCTTGTGGACAGCCAGTGTACAGCAGCCTG
  AAAAAGGGCAAGAAATGCAGCAAGACCAAGAAATCCCCCGAACCAGTCAGGTTTACTTAC
  GCTGGATGTTTGAGTGTGAAGAAATACCGGCCCAAGTACTGCGGTTCCTGCGTGGACGGC
  CGATGCTGCACGCCCCAGCTGACCAGGACTGTGAAGATGCGGTTCCGCTGCGAAGATGGG
  GAGACATTTTCCAAGAACGTCATGATGATCCAGTCCTGCAAATGCAACTACAACTGCCCG
  CATGCCAATGAAGCAGCGTTTCCCTTCTACAGGCTGTTCAATGACATTCACAAATTTAGG
  GACTAA
  
  Clone variation with respect to NM_001554.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001554 unedited
  NGGCGGTCAAAATTGTATACGACTCCTATAGGCGGCCCGCGAATCGGCACCAGGCGCCCC
  CGAGCAGCGCCCGCGCCCTCCGCGCCTTCTCCGCCGGGACCTCGAGCGAAAGACGCCCGC
  CCGCCGCCCAGCCCTCGCCTCCCTGCCCACCGGGCACACCGCGCCGCCACCCCGACCCCG
  CTGCGCACGGCCTGTCCGCTGCACACCAGCTTGTTGGCGTCTTCGTCGCCGCGCTCGCCC
  CGGGCTACTCCTGCGCGCCACAATGAGCTCCCGCATCGCCAGGGCGCTCGCCTTAGTCGT
  CACCCTTCTCCACTTGACCAGGCTGGCGCTCTCCACCTGCCCCGCTGCCTGCCACTGCCC
  CCTGGAGGCGCCCAAGTGCGCGCCGGGAGTCGGGCTGGTCCGGGACGGCTGCGGCTGCTG
  TAAGGTCTGCGCCAAGCAGCTCAACGAGGACTGCAGCAAAACGCAGCCCTGCGACCACAC
  CAAGGGGCTGGAATGCAACTTCGGCGCCAGCTCCACCGCTCTGAAGGGGATCTGCAGAGC
  TCAGTCAGAGGGCAGACCCTGTGAATATAACTCCAGAATCTACCAAAACGGGGAAAGTTT
  CCAGCCCAACTGTAAACATCAGTGCACATGTATTGATGGCGCCGTGGGCTGCATTCCTCT
  GTGTCCCCAAGAACTATCTCTCCCCAACTTGGGCTGTCCCAACCCTCGGCTGGTCAAAGT
  TACCGGGCAGTGCTGCGAGGAGTGGGTCTGTGACGAGGATAGTATCAAGGACCCCATGGN
  AGACCAGGACNGCCTCCTTGGCAGGAGCTGGGATTCGATGCCTCCGAGGTGGAGTTGACG
  AGAACAATGAATGATNGCAGTTGGAAAAGGCAGCTCACTGAAGCGGCTCCCTGGNTTTGG
  AATGGAGCTCGCATCTATACACC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001554 unedited
  AGGNANAGCACTGGGGNAGGGTCACAGGCTGCCACCCGGGTATCTGTTGAGGCAAACAGC
  TATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTAGGTAAAT
  ATTTCTTTATTAAATGGTTAATTCCATAAATAAAATCTTTTCACTGAATATAAAATTAAA
  ATCATTTACAAATTATTCCAGTATTACATTTCCCCTCCCTCCCCAAAAGCTACATTTTGA
  TAAATAAAACATTCAGTCTTAAAACACCTGATTTCTGTTTGCAGTTTAGAGTGCAGATAG
  CTGCCTCTCACAGACACTCATGGAGTGTCCCCCTTCAGGAAGGGATGGAATGCCCTCCCA
  TTTACTTTTGTCAAAGGACAAATAAAAGCTTTAAACTGTCCAACTAATCTTATTATCTCT
  TTTACGACTGTAGAACCCCAAAAGGAAAAAAGCCTAGAGAAAATATGCTTAGCATATAAT
  TTACAAACAGAAAACAGAATGTCATCAACTCCACAAGCTCCAAAATGAAGCAGTAACATG
  TGCTCCAATACTATGAAGCAAAGTATTCTCCAATCGTGGCTGCATTAGTGTCCATCCGCA
  CCAGCACCCTTGGCAGTTTCGAAATACCTTAATGCTCCTCAAGAATGAGCAAGGCTTCCT
  TTCTACAATGAGTCCCATCACCCACACCACCCCCGCCCATTTTCTCCATGATTCTGATTC
  TGACACTCTNTCTCCTTGTTTGTCTAGGTGTGCCCTGGAAACCCAGTAGCATTTAGTCCC
  TAAATTTGTGAATGTCATTGCACAGCCTGTAGAAGGGAAACGCTGCTTATTGGCATGCGG
  GCATTTGTAGTTGCATTTGCAGGACTGGATCATCATGACGTTCTTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001554
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001554.3, NP_001545.2
• RefSeq Size: 2037 bp
• RefSeq ORF: 1146 bp
• Locus ID: 3491
• Cytogenetics: 1p22.3
• Domains: IB, tsp_1, VWC, CT, Cys_knot
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
• Gene Summary: 'The secreted protein encoded by this gene is growth factor-inducible and promotes the adhesion of endothelial cells. The encoded protein interacts with several integrins and with heparan sulfate proteoglycan. This protein also plays a role in cell proliferation, differentiation, angiogenesis, apoptosis, and extracellular matrix formation. [provided by RefSeq, Sep 2011]'