RAB27A (NM_004580) Human Untagged Clone

CAT#: SC310616

RAB27A (untagged)-Human RAB27A, member RAS oncogene family (RAB27A), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RAB27A
• Synonyms: GS2; HsT18676; RAB27; RAM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004580, the custom clone sequence may differ by one or more nucleotides
  ATGTCTGATGGAGATTATGATTACCTCATCAAGTTTTTAGCTTTGGGAGACTCTGGTGTA
  GGGAAGACCAGTGTACTTTACCAATATACAGATGGTAAATTTAACTCCAAATTTATCACA
  ACAGTGGGCATTGATTTCAGGGAAAAAAGAGTGGTGTACAGAGCCAGTGGGCCGGATGGA
  GCCACTGGCAGAGGCCAGAGAATCCACCTGCAGTTATGGGACACAGCAGGGCAGGAGAGG
  TTTCGTAGCTTAACGACAGCGTTCTTCAGAGATGCTATGGGTTTTCTTCTACTTTTTGAT
  CTGACAAATGAGCAAAGTTTCCTCAATGTCAGAAACTGGATAAGCCAGCTACAGATGCAT
  GCATATTGTGAAAACCCAGATATAGTGCTGTGTGGAAACAAGAGTGATCTGGAGGACCAG
  AGAGTAGTGAAAGAGGAGGAAGCCATAGCACTCGCAGAGAAATATGGAATCCCCTACTTT
  GAAACTAGTGCTGCCAATGGGACAAACATAAGCCAAGCAATTGAGATGCTTCTGGACCTG
  ATAATGAAGCGAATGGAACGGTGTGTGGACAAGTCCTGGATTCCTGAAGGAGTGGTGCGA
  TCAAATGGTCATGCCTCTACGGATCAGTTAAGTGAAGAAAAGGAGAAAGGGGCATGTGGC
  TGTTGA
  
• Restriction Sites: Please inquire
• ACCN: NM_004580
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004580.3, NP_004571.2
• RefSeq Size: 2824 bp
• RefSeq ORF: 666 bp
• Locus ID: 5873
• Cytogenetics: 15q21.3
• Domains: ras, RAN, RAS, RHO, RAB
• Protein Families: Druggable Genome
• Gene Summary: 'The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2, 3 and 4 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.