15 Lipoxygenase 2 (ALOX15B) (NM_001039131) Human Untagged Clone

CAT#: SC310837

ALOX15B (untagged)-Human arachidonate 15-lipoxygenase, type B (ALOX15B), transcript variant b

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ALOX15B
• Synonyms: 15-LOX-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001039131, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCGAGTTCAGGGTCAGGGTGTCCACCGGAGAAGCCTTCGGGGCTGGCACATGGGACAAAGTGTCTG
  TCAGCATCGTGGGGACCCGGGGAGAGAGCCCCCCACTGCCCCTGGACAATCTCGGCAAGGAGTTCACTGC
  GGGCGCTGAGGAGGACTTCCAGGTGACGCTCCCGGAGGACGTAGGCCGAGTGCTGCTGCTGCGCGTGCAC
  AAGGCGCCCCCAGTGCTGCCCCTGCTGGGGCCCCTGGCCCCGGATGCCTGGTTCTGCCGCTGGTTCCAGC
  TGACACCGCCGCGGGGCGGCCACCTCCTCTTCCCCTGCTACCAGTGGCTGGAGGGGGCGGGGACCCTGGT
  GCTGCAGGAGGGTACAGCCAAGGTGTCCTGGGCAGACCACCACCCTGTGCTCCAGCAACAGCGCCAGGAG
  GAGCTTCAGGCCCGGCAGGAGATGTACCAGTGGAAGGCTTACAACCCAGGTTGGCCTCACTGCCTGGATG
  AAAAGACAGTGGAAGACTTGGAGCTCAATATCAAATACTCCACAGCCAAGAATGCCAACTTTTATCTACA
  GGCTGGCTCTGCTTTTGCAGAGATGAAAATCAAGGGGTTGCTGGACCGCAAGGGGCTCTGGAGGAGTCTG
  AATGAGATGAAAAGGATCTTCAACTTCCGGAGGACCCCAGCAGCTGAGCACGCATTTGAGCACTGGCAGG
  AGGACGCCTTCTTCGCCTCCCAGTTCCTGAATGGTCTCAACCCTGTCCTGATCCGCCGCTGTCACTACCT
  CCCAAAGAACTTCCCCGTCACTGATGCCATGGTGGCCTCAGTGTTGGGTCCTGGGACCAGCTTGCAGGCT
  GAGCTAGAGAAGGGCTCCCTGTTCTTGGTGGATCACGGCATCCTCTCTGGCATCCAGACCAATGTCATTA
  ATGGGAAGCCTCAGTTCTCTGCGGCCCCAATGACCCTGCTATACCAGAGCCCAGGCTGCGGGCCGCTGCT
  GCCTCTCGCCATCCAGCTCAGCCAGACCCCCGGCCCAAACAGCCCCATCTTCCTGCCCACTGATGACAAG
  TGGGACTGGTTGCTGGCCAAGACCTGGGTGCGCAATGCCGAGTTCTCCTTCCATGAGGCCCTCACGCACC
  TGCTGCACTCACATCTGCTGCCTGAGGTCTTCACCCTGGCTACCCTGCGTCAGCTGCCCCACTGCCACCC
  TCTCTTCAAGTCCACAGGCATCGGCATTGAAGGCTTCTCTGAGTTGATACAGAGGAACATGAAGCAGCTG
  AACTATTCTCTCCTGTGTCTGCCTGAGGATATCCGGACCCGAGGAGTTGAAGACATCCCAGGCTACTACT
  ACCGTGATGATGGGATGCAGATCTGGGGTATACCCTCCTCACTGGAGACCCGGGAAGCCCTGGTGCAGTA
  TGTCACCATGGTGATATTCACCTGCTCCGCCAAGCATGCGGCTGTCAGTGCAGGGCAGTTTGACTCCTGT
  GCTTGGATGCCCAACCTGCCACCCAGCATGCAGCTGCCACCACCCACCTCCAAAGGCCTGGCAACATGCG
  AGGGCTTCATAGCCACCCTCCCACCTGTCAATGCCACATGTGATGTCATCCTTGCTCTCTGGTTGCTGAG
  CAAGGAGCCTGGAGACCAAAGGCCCCTGGGCACCTATCCGGATGAGCACTTCACAGAGGAGGCCCCTCGG
  CGGAGCATCGCCACCTTCCAGAGCCGCCTGGCCCAGATCTCGAGGGGCATCCAGGAGCGGAACCAGGGCC
  TGGTGCTGCCCTACACCTACCTAGACCCTCCCCTCATCGAGAACAGCGTCTCCATCTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001039131
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039131.1, NP_001034220.1
• RefSeq Size: 2508 bp
• RefSeq ORF: 1809 bp
• Locus ID: 247
• Cytogenetics: 17p13.1
• Protein Families: Druggable Genome
• Protein Pathways: Arachidonic acid metabolism, Metabolic pathways
• Gene Summary: 'This gene encodes a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. The encoded protein converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively. This gene is located in a cluster of related genes and a pseudogene that spans approximately 100 kilobases on the short arm of chromosome 17. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (b) has multiple differences in the coding region but maintains the reading frame, compared to variant d. The encoded isoform (b) is shorter than isoform d.