TFB1M (NM_016020) Human Untagged Clone

CAT#: SC311121

TFB1M (untagged)-Human transcription factor B1, mitochondrial (TFB1M), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TFB1M
• Synonyms: CGI-75; CGI75; mtTFB; mtTFB1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC311121 sequence for NM_016020 edited (data generated by NextGen Sequencing)
  ATGGCTGCCTCCGGAAAACTCAGCACTTGCCGTCTCCCTCCGTTGCCCACGATTCGAGAA
  ATCATTAAGTTGTTAAGACTGCAAGCAGCGAAGCAGCTATCACAGAATTTCCTCCTGGAC
  TTGAGGCTGACAGATAAGATTGTAAGGAAAGCTGGCAATCTGACAAATGCTTATGTTTAC
  GAAGTGGGCCCTGGGCCAGGGGGAATCACAAGATCTATTCTTAATGCCGACGTCGCTGAA
  CTTCTGGTGGTTGAAAAGGACACTCGATTTATTCCTGGATTACAGATGCTTTCTGATGCA
  GCACCTGGGAAACTGAGAATTGTTCATGGAGATGTCTTGACATTTAAGGTAGAAAAGGCT
  TTTTCAGAAAGTCTTAAAAGACCCTGGGAAGATGATCCTCCAAATGTACATATTATTGGA
  AATCTGCCTTTTAGTGTTTCAACTCCACTGATTATCAAGTGGCTTGAAAATATTTCCTGT
  AGAGATGGACCTTTTGTTTATGGCAGAACTCAGATGACTTTGACTTTTCAAAAGGAAGTG
  GCAGAGAGACTTGCAGCCAATACAGGAAGCAAACAGCGTAGTCGCCTCTCTGTTATGGCT
  CAGTACCTCTGCAATGTTCGACACATCTTTACAATTCCAGGACAAGCTTTTGTCCCCAAA
  CCAGAGGTGGACGTGGGCGTGGTGCACTTCACTCCCTTGATACAGCCCAAGATAGAGCAG
  CCATTCAAGCTGGTGGAAAAAGTGGTTCAGAATGTATTTCAGTTCCGAAGGAAATACTGC
  CATCGAGGGCTCAGAATGTTATTCCCTGAAGCGCAGCGCTTGGAAAGCACGGGCAGGCTG
  TTAGAGTTGGCAGACATAGACCCTACTCTTCGGCCCCGCCAGCTCTCCATCTCACACTTT
  AAGAGCCTCTGTGATGTATACAGAAAAATGTGTGATGAAGACCCACAACTCTTTGCATAT
  AATTTCAGAGAAGAACTCAAGCGAAGAAAAAGCAAAAATGAAGAAAAAGAAGAGGATGAC
  GCAGAGAATTACAGACTCTAG
  
  Clone variation with respect to NM_016020.3
  633 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016020 unedited
  TACACCCGCCCGTTGTCGCATATGGGCGGTAGGCGTGTACGGTGGGCAGGTCTATATAAG
  CAGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCG
  AATTCGGCACGAGGTTGCCGCGTATCATGGCTGCCTCCGGAAAACTCAACACTTGCCGTC
  TCCCTCCGTTGCCCACGATTCGAGAAATCATTAAGTTGTTAAGACTGCAAGCAGCGAAGC
  AGCTATCACAGAATTTCCTCCTGGACTTGAGGCTGACAGATAAGATTGTAAGGAAAGCTG
  GCAATCTGACAAATGCTTATGTTTACGAAGTGGGCCCTGGGCCAGGGGGAATCACAAGAT
  CTATTCTTAATGCCGACGTCGCTGAACTTCTGGTGGTTGAAAAGGACACTCGATTTATTC
  CTGGATTACAGATGCTTTCTGATGCAGCACCTGNGAAACTGAGAATTGTTCATGGAGATG
  TCTTGACATTTAAGGTAGAAAAGGCTTTTTCAGAAAGTCTTAANAGACCCTGGGAAGATG
  ATCCTCCAAATGTACATATTATTGGAAATCTGCCTTTTAGTGTTTCAACTCCACTGATTA
  TCAAGTGGCTTGAAAATATTTCCTGTAGAGATGGACCTTTTTGTTTATGGCAGAACTCAG
  ATGACTTTGACTTTTTCAAAGGAAGTGGCAGAGAGACTTGCAGCCAATACAGGAAGCANA
  CAGCGTAATCGCCTCTCTGGTATGGCTCAGTACCTCTGCAATGTTCGCACATCTTTACAA
  TTCCAGGACAAGCTTTTGTCCCCAAACAAAGGTGAACGTGGCCGTGGTGCACTTTCACTC
  CCTGATACAGCCAAGATAGAGCAACCATTCAAGCTGGTGGAAAAAGTGGTTA
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_016020 unedited
  TCTGAACAACTCTTTGTAGCGGAGCAGCGACACCTTCAGNTGGTGGAAAAGTGGTTCAGA
  ATGTATTTCAGTTCCGAAGGAAAACTGCCATCGAGGGCTCAGAATGTTATTCCCTGAAGC
  GCAGCGCTTGGAAAGCACGGGCAGGCTGTTAGAGTTGGCAGACATAGACCCTACTCTTCG
  GCCCCGCCAGCTCTCCATCTCACACTTTAAGAGCCTCTGTGATGTATACAGAAAAATGTG
  TGATGAAGACCCACAACTCTTTGCATATAATTTCAGAGAAGAACTCAAGCGAAGAAAAAG
  CAAAAATGAAGAAAAAGAAGAGGATGACGCAGAGAATTACAGACTCTAGCTGCTGCCTGG
  GGGCGAGCAGCCTACCAGATGTCGATTTGCACTACGTGGAGCTTCTTATATAGGTACTCT
  TTTGTCTTTACAGAATGACGATACAAATGCCAATGACCAGATGTGACTTATTTTCCTTTT
  ACTATACAGCTTGGCAGAGAAAATAAATATCATCAAATAAGATACAGTACAAGCTTTCTT
  TTAATATATACTGTATAGATAGATATGACATGTTTAGTATAAACAGCATCTATTACAATC
  TTACAAAAATGAAGTATCTGCACATTTTGCCCCTAAACCACATTAAAATAACTTAAATAT
  GCTTTAAATAAAGCAAAAACTAACCAACAGCTGAAAAGAAAAGCCCAGGAGGATTAAAAA
  AATGGGCCCTTGAAAACTAATTATCACGAATTCCCCAGCCCTAACTTTTCCCCATAACCT
  AAATTATAAAAAATGGAATTTTAAAGGATCCCAAATACATTTGATACCGGCCCAAAAGGG
  GATATAAACCTAAGTAAATTACCAGATTCCTGCCCGTTTCATCTAAGGTTCCTGATCCCA
  
• Restriction Sites: Please inquire
• ACCN: NM_016020
• ORF Size: 1041 bp
• Insert Size: 1700
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_016020.1, NP_057104.1
• RefSeq Size: 1290
• RefSeq ORF: 1041
• Locus ID: 51106
• Domains: rADc
• Protein Families: Transcription Factors
• Gene Summary: The protein encoded by this gene is a dimethyltransferase that methylates the conserved stem loop of mitochondrial 12S rRNA. The encoded protein also is part of the basal mitochondrial transcription complex and is necessary for mitochondrial gene expression. The methylation and transcriptional activities of this protein are independent of one another. Variations in this gene may influence the severity of aminoglycoside-induced deafness (AID). [provided by RefSeq, Aug 2010]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.