NEK4 (NM_003157) Human Untagged Clone

CAT#: SC314802

NEK4 (untagged)-Human NIMA (never in mitosis gene a)-related kinase 4 (NEK4), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NEK4
• Synonyms: NRK2; pp12301; STK2
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003157, the custom clone sequence may differ by one or more nucleotides
  ATGCCCCTGGCCGCCTACTGCTACCTGCGGGTCGTGGGCAAGGGGAGCTATGGAGAGGTG
  ACGCTTGTGAAGCACCGGCGGGACGGCAAGCAGTATGTCATCAAAAAACTGAACCTCCGA
  AATGCCTCTAGCCGAGAGCGGCGAGCTGCTGAACAGGAAGCCCAGCTCTTGTCTCAGTTG
  AAGCATCCCAACATTGTCACCTACAAGGAGTCATGGGAAGGAGGAGATGGTCTGCTCTAC
  ATTGTCATGGGCTTCTGTGAAGGAGGTGATTTGTACCGAAAGCTCAAGGAGCAGAAAGGG
  CAGCTTCTGCCGGAGAATCAGGTGGTAGAGTGGTTTGTACAGATCGCCATGGCTTTGCAG
  TATTTACATGAAAAACACATCCTTCATCGAGATCTGAAAACTCAAAATGTCTTCCTAACA
  AGAACAAACATCATCAAAGTAGGGGACCTAGGAATTGCCCGAGTGTTGGAGAACCACTGT
  GACATGGCTAGCACCCTCATTGGCACACCCTACTACATGAGCCCTGAATTGTTCTCAAAC
  AAACCCTACAACTATAAGTCTGATGTTTGGGCTCTAGGATGCTGTGTCTATGAAATGGCC
  ACCTTGAAGCATGCTTTCAATGCAAAAGATATGAATTCTTTAGTTTATCGGATTATTGAA
  GGAAAGCTGCCAGCAATGCCAAGAGATTACAGCCCAGAGCTGGCAGAACTGATAAGAACA
  ATGCTGAGCAAAAGGCCTGAAGAAAGGCCGTCTGTGAGGAGCATCCTGAGGCAGCCTTAT
  ATAAAGCGGCAAATCTCCTTCTTTTTGGAGGCCACAAAGATAAAAACCTCCAAAAATAAC
  ATTAAAAATGGTGACTCTCAATCCAAGCCTTTTGCTACAGTGGTTTCTGGAGAGGCAGAA
  TCAAATCATGAAGTAATCCACCCCCAACCACTCTCTTCTGAGGGCTCCCAGACATATATA
  ATGGGTGAAGGCAAATGTTTGTCCCAGGAGAAACCCAGGGCCTCTGGTCTCTTGAAGTCA
  CCTGCCAGTCTGAAAGCCCATACCTGCAAACAGGACTTGAGCAATACCACAGAACTAGCC
  ACAATCAGTAGCGTAAATATTGACATCTTACCTGCAAAAGGGAGGGATTCAGTGAGTGAT
  GGCTTTGTTCAGGAGAATCAGCCAAGATATTTGGATGCCTCTAATGAGTTAGGAGGTATA
  TGCAGTATTTCTCAAGTGGAAGAGGAGATGCTGCAGGACAACACTAAATCCAGTGCCCAG
  CCTGAAAACCTGATTCCCATGTGGTCCTCTGACATTGTCACTGGGGAAAAGAATGAACCA
  GTGAAGCCTCTGCAGCCCCTAATCAAAGAACAAAAGCCAAAGGACCAGAGTCTTGCCCTG
  TCGCCCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACAGCAACCTCCGCCTCCTGGGT
  TCAAGTGATTCTCCAGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCGTGTGCCACCAC
  GCCCAGGATCAAGTTGCTGGTGAATGTATTATAGAAAAACAGGGCAGAATCCACCCAGAT
  TTACAGCCACACAACTCTGGGTCTGAACCTTCCCTGTCTCGACAGCGACGGCAAAAGAGG
  AGAGAACAGACTGAGCACAGAGGGGAAAAGAGACAGGTCCGCAGAGATCTCTTTGCTTTC
  CAAGAGTCGCCTCCTCGATTTTTGCCTTCTCATCCCATTGTTGGGAAAGTGGATGTCACA
  TCAACACAAAAAGAGGCTGAAAACCAACGTAGAGTGGTCACTGGGTCTGTGAGCAGTTCA
  AGGAGCAGTGAGATGTCATCATCAAAGGATCGACCATTATCAGCCAGAGAGAGGAGGCGA
  CTAAAGCAGTCACAGGAAGAAATGTCCTCTTCAGGCCCTTCAGTGAGGAAAGCGTCTCTG
  AGTGTAGCAGGGCCAGGAAAACCCCAGGAAGAAGACCAGCCCTTGCCTGCCCGACGGCTC
  TCCTCTGACTGCAGCGTCACTCAGGAAAGGAAACAGATTCATTGTCTGTCTGAGGATGAG
  TTAAGTTCTTCTACAAGTTCAACTGATAAGTCAGATGGGGATTACGGGGAAGGGAAAGGT
  CAGACAAATGAAATTAATGCCTTGGTACAATTGATGACTCAGACCCTGAAACTGGATTCT
  AAAGAGAGCTGTGAAGATGTCCCGGTAGCAAACCCAGTGTCAGAATTCAAACTTCATCGG
  AAATATCGGGACACACTGATACTTCATGGGAAGGTTGCAGAAGAGGCAGAGGAAATCCAT
  TTTAAAGAGCTACCTTCAGCTATTATGCCAGGTTCTGAAAAGATCAGGAGACTAGTTGAA
  GTCTTGAGAACTGATGTAATTCGTGGCCTGGGAGTTCAGCTTTTAGAGCAGGTGTATGAT
  CTTTTGGAGGAGGAGGATGAATTTGATAGAGAGGTACGTTTGCGGGAGCACATGGGTGAA
  AAGTATACAACTTACAGTGTGAAAGCTCGCCAGTTGAAATTTTTTGAAGAAAACATGAAT
  TTT
  
• Restriction Sites: Please inquire
• ACCN: NM_003157
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003157.1, NP_003148.1
• RefSeq Size: 3698 bp
• RefSeq ORF: 2526 bp
• Locus ID: 6787
• Cytogenetics: 3p21.1
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: 'The protein encoded by this gene is a serine/threonine protein kinase required for normal entry into replicative senescence. The encoded protein also is involved in cell cycle arrest in response to double-stranded DNA damage. Finally, this protein plays a role in maintaining cilium integrity, and defects in this gene have been associated with ciliopathies. [provided by RefSeq, Jan 2017]'
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.