NEK4 (NM_003157) Human Untagged Clone

CAT#: SC323353

NEK4 (untagged)-Kinase deficient mutant (K35M) of Human NIMA (never in mitosis gene a)-related kinase 4 (NEK4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NEK4
• Synonyms: NRK2; pp12301; STK2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323353 sequence for NM_003157 edited (data generated by NextGen Sequencing)
  ATGCCCCTGGCCGCCTACTGCTACCTGCGGGTCGTGGGCAAGGGGAGCTATGGAGAGGTG
  ACGCTTGTGAAGCACCGGCGGGACGGCAAGCAGTATGTCATCATGAAACTGAACCTCCGA
  AATGCCTCTAGCCGAGAGCGGCGAGCTGCTGAACAGGAAGCCCAGCTCTTGTCTCAGTTG
  AAGCATCCCAACATTGTCACCTACAAGGAGTCATGGGAAGGAGGAGATGGTCTGCTCTAC
  ATTGTCATGGGCTTCTGTGAAGGAGGTGATTTGTACCGAAAGCTCAAGGAGCAGAAAGGG
  CAGCTTCTGCCTGAGAATCAGGTGGTAGAGTGGTTTGTACAGATCGCCATGGCTTTGCAG
  TATTTACATGAAAAACACATCCTTCATCGAGATCTGAAAACTCAAAATGTCTTCCTAACA
  AGAACAAACATCATCAAAGTAGGGGACCTAGGAATTGCCCGAGTGTTAGAGAACCACTGT
  GACATGGCTAGCACCCTCATTGGCACACCCTACTACATGAGCCCTGAATTGTTCTCAAAC
  AAACCCTACAACTATAAGTCTGATGTTTGGGCTCTAGGATGCTGTGTCTATGAAATGGCC
  ACCTTGAAGCATGCTTTCAATGCAAAAGATATGAATTCTTTAGTTTATCGGATTATTGAA
  GGAAAGCTGCCACCAATGCCAAGAGATTACAGCCCAGAGCTGGCAGAACTGATAAGAACA
  ATGCTGAGCAAAAGGCCTGAAGAAAGGCCGTCTGTGAGGAGCATCCTGAGGCAGCCTTAT
  ATAAAGCGGCAAATCTCCTTCTTTTTGGAGGCCACAAAGATAAAAACCTCCAAAAATAAC
  ATTAAAAATGGTGACTCTCAATCCAAGCCTTTTGCTACAGTGGTTTCTGGAGAGGCAGAA
  TCAAATCATGAAGTAATCCACCCCCAACCACTCTCTTCTGAGGGCTCCCAGACATATATA
  ATGGGTGAAGGCAAATGTTTGTCCCAGGAGAAACCCAGGGCCTCTGGTCTCTTGAAGTCA
  CCTGCCAGTCTGAAAGCCCATACCTGCAAACAGGACTTGAGCAATACCACAGAACTAGCC
  ACAATCAGTAGCGTAAATATTGACATCTTACCTGCAAAAGGGAGGGATTCAGTGAGTGAT
  GGCTTTGTTCAGGAGAATCAGCCAAGATATTTGGATGCCTCTAATGAGTTAGGAGGTATA
  TGCAGTATTTCTCAAGTGGAAGAGGAGATGCTGCAGGACAACACTAAATCCAGTGCCCAG
  CCTGAAAACCTGATTCCCATGTGGTCCTCTGACATTGTCACTGGGGAAAAGAATGAACCA
  GTGAAGCCTCTGCAGCCCCTAATCAAAGAACAAAAGCCAAAGGACCAGAGTCTTGCCCTG
  TCGCCCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACAGCAACCTCCGCCTCCTGGGT
  TCAAGTGATTCTCCAGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCGTGTGCCACCAC
  GCCCAGGATCAAGTTGCTGGTGAATGTATTATAGAAAAACAGGGCAGAATCCACCCAGAT
  TTACAGCCACACAACTCTGGGTCTGAACCTTCCCTGTCTCGACAGCGACGGCAAAAGAGG
  AGAGAACAGACTGAGCACAGAGGGGAAAAGAGACAGGTCCGCAGAGATCTCTTTGCTTTC
  CAAGAGTCGCCTCCTCGATTTTTGCCTTCTCATCCCATTGTTGGGAAAGTGGATGTCACA
  TCAACACAAAAAGAGGCTGAAAACCAACGTAGAGTGGTCACTGGGTCTGTGAGCAGTTCA
  AGGAGCAGTGAGATGTCATCATCAAAGGATCGACCATTATCAGCCAGAGAGAGGAGGCGA
  CTAAAGCAGTCACAGGAAGAAATGTCCTCTTCAGGCCCTTCAGTGAGGAAAGCGTCTCTG
  AGTGTAGCAGGGCCAGGAAAACCCCAGGAAGAAGACCAGCCCTTGCCTGCCCGACGGCTC
  TCCTCTGACTGCAGCGTCACTCAGGAAAGGAAACAGATTCATTGTCTGTCTGAGGATGAG
  TTAAGTTCTTCTACAAGTTCAACTGATAAGTCAGATGGGGATTACGGGGAAGGGAAAGGT
  CAGACAAATGAAATTAATGCCTTGGTACAATTGATGACTCAGACCCTGAAACTGGATTCT
  AAAGAGAGCTGTGAAGATGTCCCGGTAGCAAACCCAGTGTCAGAATTCAAACTTCATCGG
  AAATATCGGGACACACTGATACTTCATGGGAAGGTTGCAGAAGAGGCAGAGGAAATCCAT
  TTTAAAGAGCTACCTTCAGCTATTATGCCAGGTTCTGAAAAGATCAGGAGACTAGTTGAA
  GTCTTGAGAACTGATGTAATTCGTGGCCTGGGAGTTCAGCTTTTAGAGCAGGTGTATGAT
  CTTTTGGAGGAGGAGGATGAATTTGATAGAGAGGTACGTTTGCGGGAGCACATGGGTGAA
  AAGTATACAACTTACAGTGTGAAAGCTCGCCAGTTGAAATTTTTTGAAGAAAACATGAAT
  TTTTGA
  
  Clone variation with respect to NM_003157.4
  104 a=>t;105 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_003157 unedited
  CCCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGGGCCCGGCTGC
  CTCAACAGCCGCCCCCACTGCCCCCTCTCGGGCATGAACCGAGCTTCTTGTTGCCGCCCGCTGCCCTACC
  CGCCGCTGCCGCCGCATCCCGACTCTGGGCCAGCGCTGGGAACATGCCCCTGGCCGCCTACTGCTACCTG
  CGGGTCGTGGGCAAGGGGAGCTATGGAGAGGTGACGCTTGTGAAGCACCGGCGGGGACGGCCAAGGCAGT
  GATGTCATCATGAAACTGAACCTCCGAAATGCCTCTAGCCCGAGAGCGGGCGAGCTGCTTGACCAGGAGG
  CCAACCTCTTGTCCTCAGTTGAGCCATCCCACCTTTGTCCCCTCCAAGGATTCAGGGAAAGGAGGGAAAG
  GGTCGGTCTACCATTGTAAGGGGCTTTGGTAAAGGAGGTGATTGGAACGAAAACTTCAGGGACCAAAAGG
  GGGACCTTCTGCCTGAAAACCAGGGGTTAGAGGGTTTTGTACAGATGCCTGGGCCTTCATATATTTATGT
  GAAAAACACTTCTTTCTCAGATATCGAGAACTCTCAAGTGTTTTCTACGAACCACACCTCTCAGTTGGGG
  CCCTGGATTTGCCCATGTGTAGAACCCTGTGAATGGGAGACCCATTGCCCCCTATAGAGACCGAGTGTGT
  CTACCCACCCACCCTAATCTGAAGTTGGGCTTACAGCTCGTCTAAAAGGCACCTTAGACTGCTCTATGCA
  AGAATGAGTCTCTCGTATCGGCATGAGGAGTGCTCATGTGCGGGAATCGTCCAACTGTCCCAGTAGTACT
  CCCTGACCCAGCGTCGAAGGCGCCT
  
  Kinase domain sequence

  >SC323353 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CCYTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
  AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGGGCCCGGCTGCCTCAA
  CAGCCGCCCCCACTGCCCCCTCTCGGGCATGAACCGAGCTTCTTGTTGCCGCCCGCTGCCCTACCCGCCG
  CTGCCGCCGCATCCCGACTCTGGGCCAGCGCTGGGAACATGCCCC
  
• Restriction Sites: Please inquire
• ACCN: NM_003157
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003157.1, NP_003148.1
• RefSeq Size: 3698 bp
• RefSeq ORF: 2526 bp
• Locus ID: 6787
• Cytogenetics: 3p21.1
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: 'The protein encoded by this gene is a serine/threonine protein kinase required for normal entry into replicative senescence. The encoded protein also is involved in cell cycle arrest in response to double-stranded DNA damage. Finally, this protein plays a role in maintaining cilium integrity, and defects in this gene have been associated with ciliopathies. [provided by RefSeq, Jan 2017]'
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.