UBF1 (UBTF) (NM_001076684) Human Untagged Clone

CAT#: SC315448

UBTF (untagged)-Human upstream binding transcription factor, RNA polymerase I (UBTF), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UBTF
• Synonyms: CONDBA; NOR-90; UBF; UBF-1; UBF1; UBF2
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001076684 edited
  ATGAACGGAGAAGCCGACTGCCCCACAGACCTGGAAATGGCCGCCCCCAAAGGCCAAGAC
  CGTTGGTCCCAGGAAGACATGCTGACTTTGCTGGAATGCATGAAGAACAACCTTCCATCC
  AATGACAGCTCCAAGTTCAAAACCACCGAATCACACATGGACTGGGAAAAAGTAGCATTT
  AAAGACTTTTCTGGAGACATGTGCAAGCTCAAATGGGTGGAGATTTCTAATGAGGTGAGG
  AAGTTCCGTACATTGACAGAATTGATCCTCGATGCTCAGGAACATGTTAAAAATCCTTAC
  AAAGGCAAAAAACTCAAGAAACACCCAGACTTCCCAAAGAAGCCCCTGACCCCTTATTTC
  CGCTTCTTCATGGAGAAGCGGGCCAAGTATGCGAAACTCCACCCTGAGATGAGCAACCTG
  GACCTAACCAAGATTCTGTCCAAGAAATACAAGGAGCTTCCGGAGAAGAAGAAGATGAAA
  TATATTCAGGACTTCCAGAGAGAGAAACAGGAGTTCGAGCGAAACCTGGCCCGATTCAGG
  GAGGATCACCCCGACCTAATCCAGAATGCCAAGAAATCGGACATCCCAGAGAAGCCCAAA
  ACCCCCCAGCAGCTGTGGTACACCCACGAGAAGAAGGTGTATCTCAAAGTGCGGCCAGAT
  GAGATCATGAGAGACTATATCCAGAAGCACCCAGAGCTGAACATCAGTGAGGAGGGTATC
  ACCAAGTCCACCCTCACCAAGGCCGAACGCCAGCTCAAGGACAAGTTTGACGGGCGACCC
  ACCAAGCCACCTCCGAACAGCTACTCGCTGTACTGCGCAGAGCTCATGGCCAACATGAAG
  GACGTGCCCAGCACAGAGCGCATGGTGCTGTGCAGCCAGCAGTGGAAGCTGCTGTCCCAA
  AAGGAGAAGGACGCCTATCACAAGAAGTGTGATCAGAAAAAGAAAGATTACGAGGTGGAG
  CTGCTCCGTTTCCTCGAGAGCCTGCCTGAGGAGGAGCAGCAGCGGGTCTTGGGGGAAGAG
  AAGATGCTGAACATCAACAAGAAGCAGGCCACCAGCCCCGCCTCCAAGAAGCCAGCCCAG
  GAAGGGGGCAAGGGCGGCTCCGAGAAGCCCAAGCGGCCCGTGTCGGCCATGTTCATCTTC
  TCGGAGGAGAAACGGCGGCAGCTGCAGGAGGAGCGGCCTGAGCTCTCCGAGAGCGAGCTG
  ACCCGCCTGCTGGCCCGAATGTGGAACGACCTGTCTGAGAAGAAGAAGGCCAAGTACAAG
  GCCCGAGAGGCGGCGCTCAAGGCTCAGTCGGAGAGGAAGCCCGGCGGGGAGCGCGAGGAA
  CGGGGCAAGCTGCCCGAGTCCCCCAAAAGAGCTGAGGAGATCTGGCAACAGAGCGTTATC
  GGCGACTACCTGGCCCGCTTCAAGAATGACCGGGTGAAGGCCTTGAAAGCCATGGAAATG
  ACCTGGAATAACATGGAAAAGAAGGAGAAACTGATGTGGATTAAGAAGGCAGCCGAAGAC
  CAAAAGCGATATGAGAGAGAGCTGAGTGAGATGCGGGCACCTCCAGCTGCTACAAATTCT
  TCCAAGAAGATGAAATTCCAGGGAGAACCCAAGAAGCCTCCCATGAACGGTTACCAGAAG
  TTCTCCCAGGAGCTGCTGTCCAATGGGGAGCTGAACCACCTGCCGCTGAAGGAGCGCATG
  GTGGAGATCGGCAGTCGCTGGCAGCGCATCTCCCAGAGCCAGAAGGAGCACTACAAAAAG
  CTGGCCGAGGAGCAGCAAAAGCAGTACAAGGTGCACCTGGACCTCTGGGTTAAGAGCCTG
  TCTCCCCAGGACCGTGCAGCATATAAAGAGTACATCTCCAATAAACGTAAGAGCATGACC
  AAGCTGCGAGGCCCAAACCCCAAATCCAGCCGGACTACTCTGCAGTCCAAGTCGGAGTCC
  GAGGAGGATGATGAAGAGGATGAGGATGACGAGGACGAGGATGAAGAAGAGGAAGATGAT
  GAGAATGGGGACTCCTCTGAAGATGGCGGCGACTCCTCTGAGTCCAGCAGCGAGGACGAG
  AGCGAGGATGGGGATGAGAATGAAGAGGATGACGAGGACGAAGACGACGACGAGGATGAC
  GATGAGGATGAAGATAATGAGTCCGAGGGCAGCAGCTCCAGCTCCTCCTCCTCAGGGGAC
  TCCTCAGACTCTGACTCCAACTGA
  
• Restriction Sites: Please inquire
• ACCN: NM_001076684
• Insert Size: 2200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001076684.1, NP_001070152.1
• RefSeq Size: 4604 bp
• RefSeq ORF: 2184 bp
• Locus ID: 7343
• Cytogenetics: 17q21.31
• Protein Families: Transcription Factors
• Gene Summary: 'This gene encodes a member of the HMG-box DNA-binding protein family. The encoded protein plays a critical role in ribosomal RNA transcription as a key component of the pre-initiation complex, mediating the recruitment of RNA polymerase I to rDNA promoter regions. The encoded protein may also play important roles in chromatin remodeling and pre-rRNA processing, and its activity is regulated by both phosphorylation and acetylation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3, 11 and X and the long arm of chromosome 11. [provided by RefSeq, Aug 2011]'
  Transcript Variant: This variant (3) lacks an exon in the coding region, but maintains the reading frame, compared to variant 1. Variants 2 and 3 encode the same isoform (b), which is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.