UBF1 (UBTF) (NM_001076683) Human Untagged Clone

CAT#: SC315449

UBTF (untagged)-Human upstream binding transcription factor, RNA polymerase I (UBTF), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UBTF
• Synonyms: CONDBA; NOR-90; UBF; UBF-1; UBF1; UBF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001076683, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAACGGAGAAGCCGACTGCCCCACAGACCTGGAAATGGCCGCCCCCAAAGGCCAAGACCGTTGGTCCC
  AGGAAGACATGCTGACTTTGCTGGAATGCATGAAGAACAACCTTCCATCCAATGACAGCTCCAAGTTCAA
  AACCACCGAATCACACATGGACTGGGAAAAAGTAGCATTTAAAGACTTTTCTGGAGACATGTGCAAGCTC
  AAATGGGTGGAGATTTCTAATGAGGTGAGGAAGTTCCGTACATTGACAGAATTGATCCTCGATGCTCAGG
  AACATGTTAAAAATCCTTACAAAGGCAAAAAACTCAAGAAACACCCAGACTTCCCAAAGAAGCCCCTGAC
  CCCTTATTTCCGCTTCTTCATGGAGAAGCGGGCCAAGTATGCGAAACTCCACCCTGAGATGAGCAACCTG
  GACCTAACCAAGATTCTGTCCAAGAAATACAAGGAGCTTCCGGAGAAGAAGAAGATGAAATATATTCAGG
  ACTTCCAGAGAGAGAAACAGGAGTTCGAGCGAAACCTGGCCCGATTCAGGGAGGATCACCCCGACCTAAT
  CCAGAATGCCAAGAAATCGGACATCCCAGAGAAGCCCAAAACCCCCCAGCAGCTGTGGTACACCCACGAG
  AAGAAGGTGTATCTCAAAGTGCGGCCAGATGAGATCATGAGAGACTATATCCAGAAGCACCCAGAGCTGA
  ACATCAGTGAGGAGGGTATCACCAAGTCCACCCTCACCAAGGCCGAACGCCAGCTCAAGGACAAGTTTGA
  CGGGCGACCCACCAAGCCACCTCCGAACAGCTACTCGCTGTACTGCGCAGAGCTCATGGCCAACATGAAG
  GACGTGCCCAGCACAGAGCGCATGGTGCTGTGCAGCCAGCAGTGGAAGCTGCTGTCCCAGAAGGAGAAGG
  ACGCCTATCACAAGAAGTGTGATCAGAAAAAGAAAGATTACGAGGTGGAGCTGCTCCGTTTCCTCGAGAG
  CCTGCCTGAGGAGGAGCAGCAGCGGGTCTTGGGGGAAGAGAAGATGCTGAACATCAACAAGAAGCAGGCC
  ACCAGCCCCGCCTCCAAGAAGCCAGCCCAGGAAGGGGGCAAGGGCGGCTCCGAGAAGCCCAAGCGGCCCG
  TGTCGGCCATGTTCATCTTCTCGGAGGAGAAACGGCGGCAGCTGCAGGAGGAGCGGCCTGAGCTCTCCGA
  GAGCGAGCTGACCCGCCTGCTGGCCCGAATGTGGAACGACCTGTCTGAGAAGAAGAAGGCCAAGTACAAG
  GCCCGAGAGGCGGCGCTCAAGGCTCAGTCGGAGAGGAAGCCCGGCGGGGAGCGCGAGGAACGGGGCAAGC
  TGCCCGAGTCCCCCAAAAGAGCTGAGGAGATCTGGCAACAGAGCGTTATCGGCGACTACCTGGCCCGCTT
  CAAGAATGACCGGGTGAAGGCCTTGAAAGCCATGGAAATGACCTGGAATAACATGGAAAAGAAGGAGAAA
  CTGATGTGGATTAAGAAGGCAGCCGAAGACCAAAAGCGATATGAGAGAGAGCTGAGTGAGATGCGGGCAC
  CTCCAGCTGCTACAAATTCTTCCAAGAAGATGAAATTCCAGGGAGAACCCAAGAAGCCTCCCATGAACGG
  TTACCAGAAGTTCTCCCAGGAGCTGCTGTCCAATGGGGAGCTGAACCACCTGCCGCTGAAGGAGCGCATG
  GTGGAGATCGGCAGTCGCTGGCAGCGCATCTCCCAGAGCCAGAAGGAGCACTACAAAAAGCTGGCCGAGG
  AGCAGCAAAAGCAGTACAAGGTGCACCTGGACCTCTGGGTTAAGAGCCTGTCTCCCCAGGACCGTGCAGC
  ATATAAAGAGTACATCTCCAATAAACGTAAGAGCATGACCAAGCTGCGAGGCCCAAACCCCAAATCCAGC
  CGGACTACTCTGCAGTCCAAGTCGGAGTCCGAGGAGGATGATGAAGAGGATGAGGATGACGAGGACGAGG
  ATGAAGAAGAGGAAGATGATGAGAATGGGGACTCCTCTGAAGATGGCGGCGACTCCTCTGAGTCCAGCAG
  CGAGGACGAGAGCGAGGATGGGGATGAGAATGAAGAGGATGACGAGGACGAAGACGACGACGAGGATGAC
  GATGAGGATGAAGATAATGAGTCCGAGGGCAGCAGCTCCAGCTCCTCCTCCTCAGGGGACTCCTCAGACT
  CTGACTCCAACTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001076683
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001076683.1, NP_001070151.1
• RefSeq Size: 4635 bp
• RefSeq ORF: 2184 bp
• Locus ID: 7343
• Cytogenetics: 17q21.31
• Protein Families: Transcription Factors
• Gene Summary: 'This gene encodes a member of the HMG-box DNA-binding protein family. The encoded protein plays a critical role in ribosomal RNA transcription as a key component of the pre-initiation complex, mediating the recruitment of RNA polymerase I to rDNA promoter regions. The encoded protein may also play important roles in chromatin remodeling and pre-rRNA processing, and its activity is regulated by both phosphorylation and acetylation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3, 11 and X and the long arm of chromosome 11. [provided by RefSeq, Aug 2011]'
  Transcript Variant: This variant (2) differs in the 5' UTR and lacks an exon in the coding region, but maintains the reading frame, compared to variant 1. Variants 2 and 3 encode the same isoform (b), which is shorter than isoform a.