IL18 (NM_001562) Human Untagged Clone

CAT#: SC315485

IL18 (untagged)-Human interleukin 18 (interferon-gamma-inducing factor) (IL18)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IL18
• Synonyms: IGIF; IL-1g; IL-18; IL1F4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001562 edited
  CTGGACAGTCAGCAAGGAATTGTCTCCCAGTGCATTTTGCCCTCCTGGCTGCCAACTCTG
  GCTGCTAAAGCGGCTGCCACCTGCTGCAGTCTACACAGCTTCGGGAAGAGGAAAGGAACC
  TCAGACCTTCCAGATCGCTTCCTCTCGCAACAAACTATTTGTCGCAGGAATAAAGATGGC
  TGCTGAACCAGTAGAAGACAATTGCATCAACTTTGTGGCAATGAAATTTATTGACAATAC
  GCTTTACTTTATAGCTGAAGATGATGAAAACCTGGAATCAGATTACTTTGGCAAGCTTGA
  ATCTAAATTATCAGTCATAAGAAATTTGAATGACCAAGTTCTCTTCATTGACCAAGGAAA
  TCGGCCTCTATTTGAAGATATGACTGATTCTGACTGTAGAGATAATGCACCCCGGACCAT
  ATTTATTATAAGTATGTATAAAGATAGCCAGCCTAGAGGTATGGCTGTAACTATCTCTGT
  GAAGTGTGAGAAAATTTCAACTCTCTCCTGTGAGAACAAAATTATTTCCTTTAAGGAAAT
  GAATCCTCCTGATAACATCAAGGATACAAAAAGTGACATCATATTCTTTCAGAGAAGTGT
  CCCAGGACATGATAATAAGATGCAATTTGAATCTTCATCATACGAAGGATACTTTCTAGC
  TTGTGAAAAAGAGAGAGACCTTTTTAAACTCATTTTGAAAAAAGAGGATGAATTGGGGGA
  TAGATCTATAATGTTCACTGTTCAAAACGAAGACTAGCTATTAAAATTTCATGCCGGGCG
  CAGTGGCTCACGCCTGTAATCCCAGCCCTTTGGGAGGCTGAGGCGGGCAGATCACCAGAG
  GTCAGGTGTTCAAGACCAGCCTGACCAACATGGTGAAACCTTCATCTCTACTAAAAATAC
  AAAAAATTAGCTGAGTGTAGTGACCCATGCCCTCAATCCCGGCTACTCAAGAGGCTGAGG
  CAGGAGAATCACTTGCACTCCGGAGGTGGAGGTTGTGGTGAGCCGAGATTGCACCATTGC
  GCTCTAGCCTGGGCAACAACAGCAAAACTCCATCTCAAAAAATAAAATAAATAAATAAAC
  AAATAAAAAATTCAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001562 unedited
  CAACACANTTCGCACGAGGCTGGACAGTTTTCAAGGAATTGTCTCCCAGTGCATTTTGCC
  CTCCTGGCTGCCAACTCTGGCTGCTAAAGCGGCTGCCACCTGCTGCAGTCTACACAGCTT
  CGGGAAGAGGAAAGGAACCTCAGACCTTCCAGATCGCTTCCTCTCGCAACAAACTATTTG
  NCTCAGTGATAAAGATGGCTGCTGAACCAGTAGAAGACAATTGCATCAACTTTGTGGCAA
  TGAAATTTATTGACAATACGCTTTACTTTATAGCTGAAGATGATGAAAACCTGGAATCAG
  ATTACTTTGGCAAGCTTGAATCTAAATTATCAGTCATAAGAAATTTGAATGACCAAGTTC
  TCTTCATTGACCAAGGAAATCGGCCTCTATTTGAAGATATGACTGATTCTGACTGTAGAG
  ATAATGCACCCCGGACCATATTTATTATAAGTATGTATAAAGATAGCCAGCCTAGAGGTA
  TGGCTGTAACTATCTCTGTGAAGTGTGAGAAAATTTCAACTCTCTCCTGTGAGAACAAAA
  TTATTTCCTTTAAGGAAATGAATCCTCCTGATAACATCAAGGATACAAAAAGTGACATCA
  TATTCTTTCAGAGGAGTGTCCCANGACATGATAATAAGATGCAATTTGAATCTTTCTCAT
  ACGAAAGATACTTTCTAGCTTGTGAAAAGAGAGAGACCTTTTAAACTCATTTTGAAAAAA
  GAGAAGAAATGGGGGATAGATCTATAATGTTCACTGGTCAAACGAAGACTAACTTTAAAA
  TTTCTGCCCGGCGCAGGGCGTTACCCCCGCAACCCCGGCCCGTTGCGGTAGCTCAGCGGG
  GGACCACTACTTATTCATGGTCGCGATGAGCCACCAGCGATCCGTTGATACCAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001562 unedited
  CTATGAACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGAATTTTTTA
  TTTGTTTATTTATTTATTTTATTTTTTGAGATGGAGTTTTGCTGTTGTTGCCCAGGCTAG
  AGCGCAATGGTGCAATCTCGGCTCACCACAACCTCCACCTCCGGAGTGCAAGTGATTCTC
  CTGCCTCAGCCTCTTGAGTAGCCGGGATTGAGGGCATGGGTCACTACACTCAGCTAATTT
  TTTGTATTTTTAGTAGAGATGAAGGTTTCACCATGTTGGTCAGGCTGGTCTTGAACACCT
  GACCTCTGGTGATCTGCCCGCCTCAGCCTCCCAAAGGGCTGGGATTACAGGCGTGAGCCA
  CTGCGCCCGGCATGAAATTTTAATAGCTAGTCTTCGTTTTGAACAGTGAACATTATAGAT
  CTATCCCCCAATTCATCCTCTTTTTTCAAAATGAGTTTAAAAAGGTCTCTCTCTTTTTCA
  CAAGCTAGAAAGTATCCTTCGTATGATGAAGATTCAAATTGCATCTTATTATCATGTCCT
  GGGACACTTCTCTGAAAGAATATGATGTCACTTTTTGTATCCTTGATGTTATCAGGAGGA
  TTCATTTCCTTAAAGGAAATAATTTTGTTCTCACAGGAGAGAGTTGAAATTTTCTCACAC
  TTCACAGAGATAGTTACAGCCATACCTCTAGGCTGGCTATCTTTATACATACTTATAATA
  AATATGGTCCCGGGTGCATTATCTCTACAGTCAGAATCAATCATATCTTCCAATAGAGGC
  CGATTTCCTTGGTCAATGAAGAGAACTTGGTCATTCAAATTTCCTATGACTGATAATTTA
  GATTCAGCTTGCCCAATAATCTGATCCCAGTTTTCATCATCCTCAGCTTTAAGAAAGCGT
  ATGTCAATAATTTCTTGGCCCAATTTATGCATTGTCTCTACTTGTTCACACCCTCCTTAT
  CCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001562
• Insert Size: 1200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_001562.2.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001562.2, NP_001553.1
• RefSeq Size: 1145 bp
• RefSeq ORF: 582 bp
• Locus ID: 3606
• Cytogenetics: 11q23.1
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Cytokine-cytokine receptor interaction, Cytosolic DNA-sensing pathway, NOD-like receptor signaling pathway
• Gene Summary: 'The protein encoded by this gene is a proinflammatory cytokine of the IL-1 family that is constitutively found as a precursor within the cytoplasm of a variety of cells including macrophages and keratinocytes. The inactive IL-18 precursor is processed to its active form by caspase-1, and is capable of stimulating interferon gamma production, and of regulating both T helper (Th) 1 and Th2 responses. This cytokine has been implicated in the injury of different organs, and in potentially fatal conditions characterized by a cytokine storm. In humans, IL-18 gene is located on chromosome 11. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2020]'
  Transcript Variant: This variant (1) represents the predominant and longer transcript, and encodes the longer isoform (1).