Signal peptide peptidase like 2B (SPPL2B) (NM_001077238) Human Untagged Clone

CAT#: SC315578

SPPL2B (untagged)-Human signal peptide peptidase-like 2B (SPPL2B), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SPPL2B
• Synonyms: IMP-4; IMP4; PSH4; PSL1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC315578 representing NM_001077238
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCAGCGGTGGCGGCTGCGCTGGCGCGGCTTTTGGCGGCCTTTCTGCTCCTCGCGGCCCAGGTGG
  CCTGTGAGTACGGCATGGTGCACGTGGTCTCCCAGGCCGGGGGCCCCGAAGGCAAAGACTACTGCATCCT
  CTACAACCCGCAGTGGGCCCATCTTCCGCACGACCTCAGCAAGGCATCTTTCCTGCAGCTGCGCAACTGG
  ACGGCCTCCCTGCTCTGCTCCGCAGCCGACCTCCCCGCCCGTGGCTTCAGCAACCAGATCCCGCTGGTGG
  CGCGGGGGAACTGCACCTTCTATGAGAAAGTGAGGCTGGCCCAGGGCAGCGGAGCACGCGGGCTGCTCAT
  CGTCAGCAGGGAGAGGCTGGTCCCCCCGGGGGGTAATAAGACGCAGTATGATGAGATTGGCATTCCCGTG
  GCCCTGCTCAGCTACAAAGACATGCTGGACATCTTCACGCGTTTCGGCCGCACGGTGAGGGCGGCGCTGT
  ATGCGCCTAAGGAGCCGGTGCTGGACTACAACATGGTCATCATCTTCATCATGGCTGTGGGCACCGTCGC
  CATCGGCGGCTACTGGGCCGGGAGTCGGGACGTGAAGAAAAGGTACATGAAGCACAAGCGCGACGATGGG
  CCCGAGAAGCAGGAGGACGAGGCGGTGGACGTGACGCCGGTGATGACCTGCGTGTTTGTGGTGATGTGCT
  GCTCCATGCTGGTGCTGCTCTACTATTTCTACGATCTCCTCGTGTACGTGGTCATCGGGATCTTCTGCCT
  GGCCTCCGCCACCGGCCTCTACAGCTGCCTGGCGCCCTGTGTGCGGCGGCTGCCCTTCGGCAAGTGCAGG
  ATCCCCAACAACAGCCTGCCCTACTTCCACAAGCGCCCGCAGGCCCGTATGCTGCTCCTGGCGCTCTTCT
  GCGTGGCCGTCAGCGTGGTGTGGGGCGTCTTCCGCAACGAGGACCAGTGGGCCTGGGTCCTCCAGGATGC
  CCTGGGCATCGCCTTCTGCCTCTACATGCTGAAGACCATCCGTCTGCCCACCTTCAAGGCCTGCACGCTG
  CTGCTGCTGGTGCTGTTCCTCTACGACATCTTCTTCGTGTTCATCACGCCCTTCCTGACCAAGAGTGGGA
  GCAGCATCATGGTGGAGGTGGCCACTGGGCCCTCGGACTCAGCCACCCGTGAGAAGCTGCCCATGGTCCT
  GAAGGTGCCCAGGCTGAACTCCTCACCTCTGGCCCTGTGTGACCGGCCCTTCTCCCTCCTGGGTTTCGGA
  GACATTTTGGTGCCAGGGCTGCTGGTGGCCTACTGCCACAGGTTTGACATCCAGGTACAGTCCTCCAGGG
  TATACTTCGTGGCCTGCACCATCGCCTATGGCGTTGGCCTCCTTGTGACATTCGTGGCACTGGCCCTGAT
  GCAGCGTGGCCAGCCCGCTCTCCTCTACCTGGTGCCCTGCACGCTGGTGACGAGCTGCGCTGTGGCGCTC
  TGGCGCCGGGAGCTGGGCGTGTTCTGGACGGGCAGCGGCTTTGCGGTGAATACCAGTTTGCTCTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001077238
• ORF Size: 1536 bp
• Insert Size: 0
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001077238.1, NP_001070706.1
• RefSeq Size: 3832
• RefSeq ORF: 1536
• Locus ID: 56928
• Protein Families: Protease, Transmembrane
• Gene Summary: This gene encodes a member of the GXGD family of aspartic proteases. The GXGD proteases are transmembrane proteins with two conserved catalytic motifs localized within the membrane-spanning regions. This enzyme localizes to endosomes, lysosomes, and the plasma membrane. It cleaves the transmembrane domain of tumor necrosis factor alpha to release the intracellular domain, which triggers cytokine expression in the innate and adaptive immunity pathways. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (3) uses an alternate splice site in the 3' coding region, compared to variant 2, resulting in an early stop codon. The encoded isoform (3) is shorter and has a distinct C-terminus compared to isoform 2.