GPD2 (NM_001083112) Human Untagged Clone

CAT#: SC316023

GPD2 (untagged)-Human glycerol-3-phosphate dehydrogenase 2 (mitochondrial) (GPD2), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GPD2
• Synonyms: GDH2; GPDM; mGPDH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001083112, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCATTTCAAAAGGCAGTGAAAGGGACGATTCTTGTTGGAGGAGGTGCTCTTGCAACTGTTTTAGGAC
  TTTCTCAGTTTGCTCATTACAGAAGGAAACAAATGAACCTGGCCTATGTTAAAGCAGCAGACTGCATTTC
  AGAACCAGTTAACAGGGAGCCTCCTTCCAGAGAAGCTCAGCTACTGACTTTGCAAAACACATCTGAATTT
  GATATCCTTGTTATTGGAGGAGGAGCAACAGGAAGTGGCTGTGCGCTAGATGCTGTCACCAGAGGACTAA
  AAACAGCCCTTGTAGAAAGAGATGATTTCTCATCAGGGACCAGCAGCAGAAGCACTAAATTGATCCATGG
  TGGTGTGAGATATCTGCAGAAGGCCATCATGAAGTTGGATATTGAGCAGTATAGGATGGTAAAAGAAGCC
  CTTCATGAGCGTGCCAACCTGCTAGAAATTGCTCCCCATTTATCAGCTCCATTGCCTATAATGCTTCCAG
  TTTACAAGTGGTGGCAGTTACCTTACTACTGGGTAGGAATCAAGCTGTATGATTTGGTTGCAGGAAGCAA
  TTGCCTAAAAAGCAGTTATGTCCTCAGCAAATCAAGAGCCCTTGAACATTTCCCAATGCTCCAGAAGGAC
  AAACTGGTAGGAGCAATTGTCTACTATGACGGACAACATAACGATGCACGGATGAACCTTGCCATTGCTC
  TGACTGCTGCCAGGTATGGGGCTGCCACAGCCAATTACATGGAGGTAGTGAGCTTGCTCAAGAAGACAGA
  CCCCCAGACAGGGAAAGTGCGTGTGAGCGGCGCACGGTGCAAGGATGTCCTCACAGGGCAGGAATTTGAC
  GTGAGAGCCAAATGTGTTATCAATGCCACGGGACCTTTCACGGACTCTGTGCGCAAAATGGATGATAAAG
  ACGCAGCAGCTATCTGCCAGCCAAGTGCTGGTGTCCATATTGTGATGCCTGGTTATTACAGCCCAGAGAG
  CATGGGACTTCTTGACCCAGCGACCAGTGATGGGCGAGTTATTTTCTTCTTACCCTGGCAAAAGATGACG
  ATCGCTGGCACTACTGATACTCCAACTGATGTTACACACCATCCAATTCCTTCAGAAGAAGATATCAACT
  TCATTTTGAATGAAGTGCGTAATTACCTGAGTTGTGATGTTGAAGTGAGAAGAGGGGATGTCCTGGCAGC
  ATGGAGTGGAATCCGTCCTCTTGTTACAGACCCCAAATCTGCAGATACTCAGTCTATCTCCCGAAATCAT
  GTTGTTGATATCAGTGAGAGTGGCCTTATTACTATAGCAGGTGGAAAGTGGACAACTTATCGGTCTATGG
  CAGAAGATACCATAAATGCTGCTGTCAAAACTCATAATTTAAAAGCAGGACCAAGTAGAACAGTTGGGCT
  TTTCCTTCAAGGGGGTAAAGATTGGAGCCCCACACTCTACATTAGGCTTGTGCAGGATTATGGACTTGAA
  AGCGAGGTGGCACAGCATCTTGCCGCCACCTATGGTGATAAGGCCTTTGAGGTGGCCAAAATGGCAAGTG
  TGACTGGCAAAAGGTGGCCTATTGTTGGAGTACGTCTTGTGTCAGAATTTCCATATATTGAAGCAGAGGT
  GAAATATGGGATTAAGGAGTATGCCTGCACTGCTGTGGATATGATTTCACGTCGTACTCGCCTGGCCTTT
  CTAAATGTCCAGGCAGCAGAGGAAGCCCTACCCAGGATTGTTGAACTGATGGGCAGGGAACTGAATTGGG
  ATGATTATAAGAAGCAGGAACAACTTGAAACAGCCAGGAAGTTTCTATATTATGAAATGGGCTATAAATC
  TCGATCAGAACAGTTAACAGATCGCTCTGAAATTAGCCTACTGCCTTCAGACATTGACAGGTATAAGAAG
  AGATTTCATAAGTTTGATGCAGACCAGAAAGGCTTTATTACCATTGTTGATGTTCAGCGTGTATTAGAGA
  GTATCAATGTCCAAATGGATGAAAATACACTCCATGAAATTCTAAATGAAGTTGATTTGAATAAAAATGG
  ACAGGTTGAACTCAATGAATTTTTGCAGCTGATGAGTGCTATTCAAAAAGGAAGGGTATCTGGAAGCCGG
  CTTGCTATACTAATGAAAACTGCAGAAGAGAACCTCGACAGAAGAGTTCCAATTCCAGTGGACCGTAGTT
  GTGGAGGATTGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001083112
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001083112.2, NP_001076581.2
• RefSeq Size: 6046 bp
• RefSeq ORF: 2184 bp
• Locus ID: 2820
• Cytogenetics: 2q24.1
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Glycerophospholipid metabolism
• Gene Summary: 'The protein encoded by this gene localizes to the inner mitochondrial membrane and catalyzes the conversion of glycerol-3-phosphate to dihydroxyacetone phosphate, using FAD as a cofactor. Along with GDP1, the encoded protein constitutes the glycerol phosphate shuttle, which reoxidizes NADH formed during glycolysis. Two transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Jan 2010]'
  Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.