COPA (NM_001098398) Human Untagged Clone

CAT#: SC316290

COPA (untagged)-Human coatomer protein complex, subunit alpha (COPA), transcript variant 1


  "NM_001098398" in other vectors (6)

Reconstitution Protocol

USD 1,100.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol COPA
Synonyms AILJK; alpha-COP; HEP-COP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>NCBI ORF sequence for NM_001098398, the custom clone sequence may differ by one or more nucleotides


ATGTTAACCAAATTCGAGACCAAGAGCGCGCGGGTCAAAGGGCTCAGCTTTCACCCCAAAAGACCTTGGA
TCCTGACTAGTTTACATAATGGGGTCATCCAGTTATGGGACTATCGGATGTGCACTCTCATTGACAAGTT
TGATGAACATGATGGTCCAGTGCGAGGCATTGACTTCCATAAGCAGCAGCCACTGTTCGTCTCTGGAGGA
GATGACTATAAGATTAAGGTTTGGAATTACAAGCTTCGGCGCTGTCTTTTCACATTGCTTGGGCACTTAG
ATTATATTCGCACCACGTTTTTTCATCATGAATATCCCTGGATTCTGAGTGCCTCCGATGATCAGACCAT
CCGAGTGTGGAACTGGCAATCTAGAACCTGTGTTTGTGTGTTAACAGGGCACAACCATTATGTGATGTGT
GCTCAGTTCCACCCCACAGAAGACTTGGTAGTATCAGCCAGCCTGGACCAGACTGTGCGCGTTTGGGATA
TTTCTGGTCTGAGGAAAAAAAACCTGTCCCCTGGTGCGGTGGAATCGGATGTGAGAGGAATAACTGGGGT
TGATCTATTTGGAACTACAGATGCAGTGGTGAAGCATGTACTAGAGGGTCACGATCGTGGAGTAAACTGG
GCTGCCTTCCACCCCACTATGCCCCTTATTGTATCTGGGGCAGATGATCGTCAAGTGAAGATCTGGCGCA
TGAATGAATCAAAGGCATGGGAGGTTGATACCTGCCGGGGCCATTACAACAATGTATCTTGTGCCGTCTT
CCACCCTCGCCAAGAGTTGATCCTCAGCAATTCTGAGGACAAGAGTATTCGAGTCTGGGATATGTCTAAG
CGGACTGGGGTTCAGACTTTCCGCAGAGACCATGATCGTTTCTGGGTCCTAGCTGCTCACCCTAACCTTA
ACCTCTTTGCAGCAGGCCATGATGGTGGTATGATTGTGTTTAAGCTGGAACGGGAACGGCCAGCCTATGC
TGTTCATGGCAATATGCTACACTATGTCAAGGACCGATTCTTACGACAGCTGGATTTCAACAGCTCCAAA
GATGTAGCTGTGATGCAGTTGCGGAGTGGTTCCAAGTTTCCAGTATTCAATATGTCATACAATCCAGCAG
AAAATGCAGTCCTGCTTTGTACAAGAGCTAGCAATCTAGAGAATAGTACCTATGACCTGTACACCATCCC
TAAAGATGCTGACTCCCAGAATCCTGATGCGCCTGAAGGGAAACGATCCTCAGGCCTGACAGCCGTTTGG
GTCGCTCGAAATCGGTTTGCTGTCCTAGATCGGATGCATTCGCTTCTGATCAAGAATCTGAAGAATGAGA
TCACCAAAAAGGTACAGGTGCCCAACTGTGATGAGATCTTCTATGCTGGCACAGGCAATCTCCTGCTTCG
AGATGCGGACTCTATCACACTCTTTGACGTACAGCAGAAGCGGACTCTGGCATCTGTGAAGATTTCTAAA
GTGAAATACGTTATCTGGTCAGCAGACATGTCACATGTAGCACTACTAGCCAAACACGAACACTCATGCC
CTTTGCCTCTTACAGCCATTGTGATCTGTAACCGCAAACTGGATGCTTTATGTAACATTCATGAGAACAT
TCGTGTCAAGAGTGGGGCCTGGGATGAGAGTGGGGTATTTATCTATACCACAAGCAACCACATCAAATAT
GCTGTCACCACTGGGGACCACGGGATCATTCGAACTCTGGATTTACCCATCTATGTCACACGGGTGAAGG
GCAACAATGTATACTGCCTAGACAGGGAGTGTCGTCCCCGGGTACTCACCATTGATCCCACTGAGTTCAA
ATTCAAGCTGGCCCTGATCAACAGAAAATATGATGAGGTACTGCACATGGTGAGGAATGCCAAACTAGTT
GGCCAGTCTATTATTGCTTATCTCCAGAAGAAGGGCTATCCTGAAGTGGCACTGCATTTTGTCAAGGATG
AGAAAACTCGCTTTAGTCTGGCACTGGAGTGTGGAAACATTGAGATTGCTCTGGAAGCAGCCAAAGCACT
GGATGACAAGAACTGCTGGGAAAAGCTGGGAGAAGTGGCCCTGCTGCAGGGGAACCACCAGATTGTGGAA
ATGTGCTATCAGCGTACCAAAAACTTTGACAAACTTTCCTTCCTGTATCTTATCACTGGCAACTTAGAAA
AACTTCGCAAGATGATGAAGATTGCTGAGATCAGAAAGGACATGAGTGGCCACTATCAGAATGCCCTATA
CCTGGGTGATGTGTCAGAGCGTGTGCGGATCCTGAAGAACTGTGGACAGAAGTCCCTGGCCTATCTCACA
GCTGCTACCCATGGCTTAGATGAAGAAGCTGAGAGCCTAAAGGAGACATTTGACCCAGAGAAGGAGACAA
TCCCAGACATTGACCCTAATGCCAAGCTGCTCCAGCCACCTGCACCTATCATGCCATTGGATACCAATTG
GCCTTTATTGACTGTATCCAAAGGATTTTTTGAAGGCACCATTGCCAGCAAAGGGAAGGGAGGAGCACTG
GCTGCTGACATTGACATTGACACTGTTGGTACAGAGGGCTGGGGAGAGGATGCAGAGCTGCAGTTGGATG
AAGATGGGTTTGTGGAGGCTACAGAAGGTTTGGGGGATGATGCTCTTGGCAAGGGACAGGAAGAAGGAGG
TGGCTGGGATGTAGAAGAAGATCTGGAGCTCCCTCCTGAGCTGGATATATCCCCTGGGGCAGCTGGTGGG
GCTGAAGATGGTTTCTTTGTGCCCCCAACCAAGGGAACAAGTCCAACTCAGATCTGGTGTAATAACTCTC
AGCTTCCAGTTGATCACATCCTGGCAGGCTCTTTCGAAACAGCCATGCGGCTCCTTCATGACCAAGTAGG
GGTAATCCAGTTTGGCCCCTACAAGCAACTGTTCCTACAGACATACGCCCGAGGCCGCACAACCTATCAG
GCTCTGCCCTGCCTACCCTCCATGTATGGCTATCCTAATCGCAACTGGAAGGATGCAGGGCTGAAGAATG
GTGTACCAGCTGTGGGCCTGAAGCTTAATGACCTCATCCAACGGTTGCAGCTGTGCTACCAGCTCACCAC
AGTTGGCAAATTTGAGGAGGCTGTGGAAAAATTCCGTTCCATCCTTCTCAGTGTGCCACTTCTTGTTGTG
GACAATAAACAAGAGATTGCAGAGGCCCAGCAGCTCATCACCATTTGCCGTGAGTACATTGTGGGTTTGT
CCGTGGAGACAGAAAGGAAGAAGCTGCCCAAAGAGACTCTAGAACAGCAGAAGCGCATCTGTGAGATGGC
AGCCTATTTCACCCACTCAAACCTGCAGCCTGTGCACATGATCCTGGTGCTGCGTACAGCCCTCAATCTG
TTCTTCAAGCTCAAGAACTTCAAGACAGCTGCCACCTTTGCTCGGCGCCTACTAGAACTCGGGCCCAAGC
CTGAGGTGGCCCAACAGACCCGAAAAATCCTGTCTGCCTGTGAGAAGAATCCCACAGATGCCTACCAGCT
CAATTATGACATGCACAACCCCTTTGACATTTGTGCTGCATCATATCGGCCCATCTACCGTGGAAAGCCA
GTAGAAAAGTGTCCACTCAGTGGGGCCTGCTATTCCCCTGAGTTCAAAGGTCAAATCTGCAGGGTCACCA
CAGTGACAGAGATTGGCAAAGATGTGATTGGTTTAAGGATCAGTCCTCTGCAGTTTCGCTAA


Restriction Sites Please inquire     
ACCN NM_001098398
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001098398.1, NP_001091868.1
RefSeq Size 5666 bp
RefSeq ORF 3702 bp
Locus ID 1314
Cytogenetics 1q23.2
Gene Summary 'In eukaryotic cells, protein transport between the endoplasmic reticulum and Golgi compartments is mediated in part by non-clathrin-coated vesicular coat proteins (COPs). Seven coat proteins have been identified, and they represent subunits of a complex known as coatomer. The subunits are designated alpha-COP, beta-COP, beta-prime-COP, gamma-COP, delta-COP, epsilon-COP, and zeta-COP. The alpha-COP, encoded by COPA, shares high sequence similarity with RET1P, the alpha subunit of the coatomer complex in yeast. Also, the N-terminal 25 amino acids of alpha-COP encode the bioactive peptide, xenin, which stimulates exocrine pancreatic secretion and may act as a gastrointestinal hormone. Alternative splicing results in multiple splice forms encoding distinct isoforms. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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