PRAS40 (AKT1S1) (NM_001098632) Human Untagged Clone

CAT#: SC316353

AKT1S1 (untagged)-Human AKT1 substrate 1 (proline-rich) (AKT1S1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AKT1S1
• Synonyms: Lobe; PRAS40
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001098632 edited
  GGATAACGGGATGAAGCCGTCAGTGCCCCCAGAAACGAAGCGGCCCCGGACGAATTTCTG
  AGTCACCGTCGCGAGAAAGCGGGCTGAGCCGCCATTTTGAAGCCTGGCAAACCGAAGCAA
  GAAATGCTGCCGTGTTGGATCTTTGCCAGCCTTCGTGCCGAATGGGAGCAGGGCGCGGAT
  GGCGTCGGGGCGCCCCGAGGAGCTGTGGGAGGCCGTGGTGGGGGCCGCTGAGCGCTTCCG
  GGCCCGGACTGGCACGGAGCTGGTGCTGCTGACCGCGGCCCCGCCGCCACCACCCCGCCC
  GGGCCCCTGTGCCTATGCTGCCCATGGTCGAGGAGCCCTGGCGGAGGCAGCGCGCCGTTG
  CCTCCACGACATCGCACTGGCCCACAGGGCTGCCACTGCTGCTCGGCCTCCTGCGCCCCC
  ACCAGCACCACAGCCACCCAGTCCCACACCCAGCCCACCCCGGCCTACCCTGGCCAGAGA
  GGACAACGAGGAGGACGAGGATGAGCCCACAGAGACAGAGACCTCCGGGGAGCAGCTGGG
  CATTAGTGATAATGGAGGGCTCTTTGTGATGGATGAGGACGCCACCCTCCAGGACCTTCC
  CCCCTTCTGTGAGTCAGACCCCGAGAGTACAGATGATGGCAGCCTGAGCGAGGAGACCCC
  CGCCGGCCCCCCCACCTGCTCAGTGCCCCCAGCCTCAGCCCTACCCACACAGCAGTACGC
  CAAGTCCCTGCCTGTGTCTGTGCCCGTCTGGGGCTTCAAGGAGAAGAGGACAGAGGCGCG
  GTCATCAGATGAGGAGAATGGGCCGCCCTCTTCGCCCGACCTGGACCGCATCGCGGCGAG
  CATGCGCGCGCTGGTGCTGCGAGAGGCCGAGGACACCCAGGTCTTCGGGGACCTGCCACG
  GCCGCGGCTTAACACCAGCGACTTCCAGAAGCTGAAGCGGAAATATTGAAGTCCAGGGAG
  GGAGCGCCCCGGGCCGCGTCCGCCCCGTCCCACACTACGCCCCCGCCCCACTCCCGGGGC
  CTGCTAATCTGAGGCCGATCCGGGACCGGCCTCCTTGCGTCTCCCATTCCCAATTGGCTC
  CAGCTTCCTGGGGGCGTGGCCAAGCCCTCCTCTTCCCAGAATTGGCCCGGGGCCTTCAAT
  TTACGTTCTTTACACTACGGGGACTGGGGTCGTCTTTGCCCACGTCCCGACAACTTGTTC
  CCTGACCCCCTCAGGGATGGCCCCAAACTGTCCCTGCCTCTGGCACCCCCTTTCATTGGT
  TCCATCCATCCCCACAACAGCCTGCCAATCGAAGCCCGTCCCTGCATCCAGGATGGTACC
  AGCTCCCGCCCCTCGCCCCCCACCTCCACAGGTGCCTTAAAGGGCCCTCGTCCACCCAAG
  GTGGGGGGCAGGGGCCCTCACTCTCCGGCCCTGGTGTGGGGGAGAGAGTGAGGGGTTGGG
  GGATCGGCAGTTGGGAGGGGCGCTCTGAGATTAAAGAGTTTTACCTCTGAGATAAAAAAA
  AAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032375 unedited
  CCCGGTCAGATTTTGTATACGACTCACTATAGGCGGACCGCGAATTCGCACGAGGGGATA
  ACGGGATGAAGCCGTCAGTGCCCCCAGAAACGAACGGCCCCGGACGAATTTCTGAGTCAC
  CGTCGCGAGAAAGCGGGCTGAGCCGCCATTTTGAAGCCTGGCAAACCGAAGCAAGAAATG
  CTGCCGTGTTGGATCTTTGCCAGCCTTCGTGCCGAATGGGAGCAGGGCGCGGATGGCGTC
  GGGGCGCCCCGAGGAGCTGTGGGAGGCCGTGGTGGGGGCCGCTGAACCCTTCCGGCCCCG
  GACTGGCACGGAGCTGGTGCTGCTGACCGCGGCCCAGTCGTCATTACCCGGGAAGGGCCC
  GAGCGCCAATGCTGACCATGGACGAGGTGTCCTGCCCAATGCAGCGGGCCGATGACTGTA
  GGCCATGGTTCTGCCCTTTTTTTTCTGTCCCCCTTTACCGCCCCCCCCCCCCCCTTTCCC
  CCCCTTTTCCCCCCCGTTTTGTATTCATTTATGCCTTGTTTTTTTTGTCCATTTTCCCCC
  TTTTTTTTCCCTCCCCTTTTTCCGTTTTCTCCCCCTTTCTTATTCTTTCGCCCACCCGTT
  TTTTTTCCCGGTCCCCCGCCCCACATTTTCCCCCCGATTTTTTTTACCCCCCCACCCCCC
  ATTCGCCCTCCCTCTTGTCCACCCGCTTGTGTTTTCTTTACTGGTTCCCCTTTTCCCCCG
  TTTTTTTATTTTTCCCCCTTTTTCCCCGCCTTTTCCCCTTTTTTTTCCCCCCCTTTTTTC
  CCCCCCTTTGTTTCCTTTTTCCCCCTTTTTTTTCCCCCGCCTTTCCCCTTTCCCCCTGTG
  TTTCCCCCCTTTTTTATTGTTTCCTTTTTTATGGTGCCCCCGCCCTCCCCCCACCCCGTT
  ATCCCCCCCCCCCCCCCCCCATT
  
• Restriction Sites: Please inquire
• ACCN: NM_001098632
• ORF Size: 771 bp
• Insert Size: 1500
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001098632.1, NP_001092102.1
• RefSeq Size: 2033
• RefSeq ORF: 771
• Locus ID: 84335
• Gene Summary: AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]). [supplied by OMIM, Mar 2008]
  Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2, 3, 4, and 5 encode the same isoform (b).